Comments on the systematics and morphology of Smithophis bicolor (Blyth, 1855) (Reptilia: Squamata: Natricidae) based on topotypical specimens from Meghalaya, India

Authors

  • Jayaditya Purkayastha Help Earth, 16, Raghunath Choudhury Path, Lachitnagar, Guwahati, Assam 781007, India.
  • Bipin Meetei Asem Zoological Survey of India, Northeastern Regional Centre, Shillong, Meghalaya 793014, India.
  • Hmar Tlawmte Lalremsanga Developmental Biology and Herpetology Laboratory, Department of Zoology, Mizoram University, Tanhril, Mizoram 796004, India.
  • Madhurima Das Help Earth, 16, Raghunath Choudhury Path, Lachitnagar, Guwahati, Assam 781007, India.
  • Holiness Warjri Department of Zoology, Assam Don Bosco University, Sonapur, Assam 781027, India.
  • Goldenstar Thongni Rohbah, South West Khasi Hills District, Mawkyrwat, Meghalaya 793114, India.
  • Sanath Chandra Bohra Help Earth, 16, Raghunath Choudhury Path, Lachitnagar, Guwahati, Assam 781007, India.

DOI:

https://doi.org/10.11609/jott.9436.17.4.26803-26813

Keywords:

Cytochrome b, hemipenis, herpetofauna, Khasi hills, Mairang, northeastern india, phylogeny, re-description, shillong plateau, taxonomy, type locality

Abstract

The Natricid Snake Smithophis bicolor has been poorly studied and understood since its initial description in the mid-1800s. This study presents a detailed morphological description of S. bicolor based on five examined specimens including a freshly collected mutilated specimen from Meghalaya State, India. For the first time, genetic data has been obtained and analysed, providing novel insights into the species’ phylogenetic position within its genus Smithophis. Morphological comparisons and molecular analyses using cytochrome b mitochondrial gene reveal subtle variations that contribute to a clearer understanding of the species’ taxonomic identity. This study allows us to conclude that currently S. bicolor is restricted to the higher elevations of Meghalaya indicating that literature records mentioning the presence of this species from Mizoram actually represents a distinct, new unnamed lineage.

Author Biographies

Jayaditya Purkayastha, Help Earth, 16, Raghunath Choudhury Path, Lachitnagar, Guwahati, Assam 781007, India.

.

Bipin Meetei Asem, Zoological Survey of India, Northeastern Regional Centre, Shillong, Meghalaya 793014, India.

.

Hmar Tlawmte Lalremsanga, Developmental Biology and Herpetology Laboratory, Department of Zoology, Mizoram University, Tanhril, Mizoram 796004, India.

.

Madhurima Das, Help Earth, 16, Raghunath Choudhury Path, Lachitnagar, Guwahati, Assam 781007, India.

.

Holiness Warjri, Department of Zoology, Assam Don Bosco University, Sonapur, Assam 781027, India.

.

Goldenstar Thongni, Rohbah, South West Khasi Hills District, Mawkyrwat, Meghalaya 793114, India.

.

Sanath Chandra Bohra, Help Earth, 16, Raghunath Choudhury Path, Lachitnagar, Guwahati, Assam 781007, India.

.

References

Agarwal, I., S. Mahony, V.B. Giri, R. Chaitanya & A.M. Bauer (2018). Six new Cyrtodactylus (Squamata: Gekkonidae) from northeast India. Zootaxa 4524(5): 501–535. https://doi.org/10.11646/zootaxa.4524.5.1

Beddome, R.H. (1863). Further notes upon the snakes of the Madras Presidency; with descriptions of new species. Madras Quarterly Journal of Medical Science 6: 41–48 (Reprint: Journal of the Society for the Bibliography of Natural Science London, 1: 306–314, 1940).

Blyth, E. (1855). Notices and descriptions of various reptiles, new or little known (part 2). Journal of Asiatic Society of Bengal, Calcutta 23(3): 287–302 (1854).

Boulenger, G.A. (1893). Catalogue of the Snakes in the British Museum. (Natural History), London (Taylor & Francis), 448 pp.

Burbrink, F.T., R. Lawson, & J.B. Slowinski (2000). Mitochondrial DNA phylogeography of the polytypic North American Rat Snake Elaphe obsoleta: a critique of the subspecies concept. Evolution 54(6): 2107–2118.

Chandramouli, S.R., P. Karthik, R.S. Naveen, S. Babu, P.V. Karunakaran & H.N. Kumara (2021). A Two-colored Forestsnake, Smithophis bicolor (Blyth 1855) (Reptilia: Natricidae), from the Khasi Hills, Meghalaya, India. Reptiles & Amphibians 28(1): 24–25. https://doi.org/10.17161/randa.v28i1.15285

Das, A., V. Deepak, A. Captain, E.O. Wade & D.J. Gower (2020). Description of a new species of Smithophis Giri et al. 2019 (Serpentes: Colubridae: Natricinae) from Arunachal Pradesh, India. Zootaxa 4860(2): 267–283. https://doi.org/10.11646/zootaxa.4860.2.8

Das, I., R.K.L. Tron, D. Rangad & R.N. Hooroo (2010). A new species of Leptolalax (Anura: Megophryidae) from the sacred groves of Mawphlang, Meghalaya, north-eastern India. Zootaxa 2339(1): 44–56. https://doi.org/10.11646/zootaxa.2339.1.2

Datta-Roy, A., I. Das, A.M. Bauer, R.K.L. Tron & P. Karanth (2013). Lizard wears shades. A spectacled Sphenomorphus (Squamata: Scincidae), from the sacred forests of Mawphlang, Meghalaya, north-east India. Zootaxa 3701(2): 257–276. https://doi.org/10.11646/zootaxa.3701.2.7

Edgar, R.C. (2004). MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32: 1792–1797. https://doi.org/10.1093/nar/gkh340

Giri, V.B., R. Chaitanya, S. Mahony, S. Lalrounga, C. Lalrinchhana, A. Das, V. Sarkar, P. Karanth & V. Deepak (2019). On the systematic status of the genus Oriocalotes Günther, 1864 (Squamata: Agamidae: Draconinae) with the description of a new species from Mizoram state, northeast India. Zootaxa 4638(4): 451–484. https://doi.org/10.11646/zootaxa.4638.4.1

Giri, V.B., V. Deepak, A. Captain, A. Das, S. Das, K.P. Rajkumar, R.L. Rathish & D.J. Gower (2017). A new species of Rhabdops Boulenger, 1893 (Serpentes: Natricinae) from the northern Western Ghats region of India. Zootaxa 4319(1): 27–52. https://doi.org/10.11646/zootaxa.4319.1.2

Giri, V.B., D.J. Gower, A. Das, H.T. Lalremsanga, S. Lalronunga, A. Captain & V. Deepak (2019). A new genus and species of natricine snake from northeast India. Zootaxa 4603: 241–264. https://doi.org/10.11646/zootaxa.4603.2.2

Kalyaanamoorthy, S., B.Q. Minh, T.K. Wong, A.V. Haeseler & L.S. Jermiin (2017). ModelFinder: fast model selection for accurate phylogenetic estimates. Nature Methods 14: 587–589. https://doi.org/10.1038/nmeth.4285

Kamei, R.G., D.J. Gower, M. Wilkinson & S.D. Biju (2013). Systematics of the caecilian family Chikilidae (Amphibia: Gymnophiona) with the description of three new species of Chikila from northeast India. Zootaxa 3666(4): 401–435. http://doi.org/10.11646/zootaxa.3666.4.1

Kumar, S., G. Stecher & K. Tamura (2016). MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33: 1870–1874. https://doi.org/10.1093/molbev/msw054

Lanfear, R., P.B. Frandsen, A.M. Wright, T. Senfeld & B. Calcott (2017). PartitionFinder 2: new methods for selecting partitioned models of evolution for molecular and morphological phylogenetic analyses. Molecular Biology and Evolution 34: 772–773. https://doi.org/10.1093/molbev/msw260

Mahony, S., R.G. Kamei, E.C. Teeling & S.D. Biju (2018). Cryptic diversity within the Megophrys major species group (Amphibia: Megophryidae) of the Asian Horned Frogs: phylogenetic perspectives and a taxonomic revision of south Asian taxa, with descriptions of four new species. Zootaxa 4523(1): 1–96. https://doi.org/10.11646/zootaxa.4523.1.1

Mahony, S., R.G. Kamei, E.C. Teeling & S.D. Biju (2020). Taxonomic review of the Asian horned frogs (Amphibia: Megophrys Kuhl & Van Hasselt) of northeast India and Bangladesh previously misidentified as M. parva (Boulenger), with descriptions of three new species. Journal of Natural History 54(1–4): 119–194. https://doi.org/10.1080/00222933.2020.1736679

Mahony, S., S. Sengupta, R.G. Kamei & S.D. Biju (2011). A new low altitude species of Megophrys Kuhl and van Hasselt (Amphibia: Megophryidae), from Assam, northeast India. Zootaxa 3059: 36–46. https://doi.org/10.11646/zootaxa.3059.1.2

Mahony, S., E.C. Teeling & S.D. Biju (2013). Three new species of Horned Frogs, Megophrys (Amphibia: Megophryidae), from northeast India, with a resolution to the identity of Megophrys boettgeri populations reported from the region. Zootaxa 3722(2): 143–169. https://doi.org/10.11646/zootaxa.3722.2.2

Minh B.Q., M.A.T. Nguyen & A.V. Haeseler (2013). Ultrafast approximation for phylogenetic bootstrap. Molecular Biology and Evolution 30: 1188–1195. https://doi.org/10.1093/molbev/mst024

Mirza, Z.A., V.K. Bhardwaj, J.C. Lalmuanawma, G. Choure, H.T. Lalremsanga, M. Vabeiryureilai, A. Captain, A. Zagade & H. Patel (2024a). A new species of Smithophis Giri et al. 2019 from the Indo-Burma Region. Diversity 16(8): 480. https://doi.org/10.3390/d16080480

Mirza, Z.A., S. Pattekar, S. Verma, B.L. Stuart, J. Purkayastha, P.P. Mohapatra & H. Patel (2024b). A new Long-snouted Vine Snake species in the genus Ahaetulla Link, 1807 (Colubridae: Chrysopeleinae) from India. Journal of Asia-Pacific Biodiversity 17(4): 696–703. https://doi.org/10.1016/j.japb.2024.04.017

Nguyen, L.T., H.A. Schmidt, A. Von Haeseler & B.Q. Minh (2015). IQ-TREE: a fast and effective stochastic algorithm for estimating maximum likelihood phylogenies. Molecular Biology and Evolution 32: 268–274. https://doi.org/10.1093/molbev/msu300

Purkayastha, J. & M. Matsui (2012). A new species of Fejervarya (Anura: Dicroglossidae) from Mawphlang, northeastern India. Asian Herpetological Research 3(1): 31–37. https://doi.org/10.3724/SP.J.1245.2012.00031

Purkayastha, J., S.C. Bohra & M. Das (2020a). First record of the Urban Bent-toed Gecko, Cyrtodactylus urbanus Purkayastha, Das, Bohra, Bauer, and Agarwal 2018 (Squamata: Gekkonidae), from Meghalaya, India. ICRF Reptiles & Amphibians Conservation and Natural History 27(3): 512–513. https://doi.org/10.17161/randa.v27i3.14901

Purkayastha, J., M. Das, S.C. Bohra, A.M. Bauer & I. Agarwal (2020b). Another new Cyrtodactylus (Squamata: Gekkonidae) from Guwahati, Assam, India. Zootaxa 4732(3): 375–392. https://doi.org/10.11646/zootaxa.4732.3.2

Purkayastha, J., H.T. Lalremsanga, S.C. Bohra, L. Biakzuala, H. Decemson, L. Muansanga, M. Vabeiryureilai, S. Chauhan & Y.S. Rathee (2021). Four new Bent-toed Geckos (Cyrtodactylus Gray: Squamata: Gekkonidae) from northeast India. Zootaxa 4980(3): 451–489. https://doi.org/10.11646/zootaxa.4980.3.2

Purkayastha, J., H.T. Lalremsanga, B. Litho, Y.S. Rathee, S.C. Bohra, M. Vabeiryureilai, L. Biakzuala & L. Muansanga (2022). Two new Cyrtodactylus (Squamata, Gekkonidae) from northeast India. European Journal of Taxonomy 794: 111–139. https://doi.org/10.5852/ejt.2022.794.1659

Rathee, Y.S., J. Purkayastha, H.T. Lalremsanga, S. Dalal, L. Biakzuala, L. Muansanga & Z.A. Mirza (2022). A new cryptic species of Green Pit Viper of the genus Trimeresurus Lacépède, 1804 (Serpentes, Viperidae) from northeast India. PLoS One 17(5): e0268402. https://doi.org/10.1371/journal.pone.0268402

Tamura, K. & M. Nei (1993). Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution 10: 512–526. https://doi.org/10.1093/oxfordjournals.molbev.a040023

Vogel, G., Z. Chen, V. Deepak, D.J. Gower, J. Shi, L.I. Ding & M. Hou (2020). A new species of the genus Smithophis (Squamata: Serpentes: Natricidae) from southwestern China and northeastern Myanmar. Zootaxa 4803(1): 51–74. https://doi.org/10.11646/zootaxa.4803.1.3

Downloads

Published

26-04-2025

Issue

Section

Articles