Efficacy of levamisole and oxyclozanide treatment on gastrointestinal nematodes of ungulates at the Central Zoo, Nepal
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Abstract
The efficacy evaluation of levamisole and oxyclozanide treatment on the gastrointestinal nematodes of ungulates at the central zoo, Nepal was carried out from June—August 2021. A total of 40 fecal samples were collected from 10 species of ungulates from the central zoo for determining the efficacy of the anthelmintic given at day 0 of pretreatment and post-treatment analysis on day 07 and day 14. The concentration method (floatation concentration) was used for the microscopic examination of eggs, and quantitative examination (EPG) of nematode eggs was carried out with the help of modified McMaster slides. The identification was done using an optic micrometer and fecal egg culture. Anthelmintic resistance status was evaluated by the Fecal Egg Count Reduction Technique (FECRT) based on the method described by the World Association for the Advancement of Veterinary Parasitology (WAAVP) guidelines and with the Bayesian hierarchical model. Out of 40 samples, nematode prevalence was found to be 68%, in which single infection was detected in 48% and double infection in 52%. The efficacy of Zanide L forte (levamisole-0.75 g and oxyclozanide-1.00 g) was found to be 85% (UI 80-89) at day 07 and 89% (UI 85-92) at day 14 by using Hierarchical Modelling of Fecal Egg count based on ‘eggCounts-2.3 on R version 3.6.1 and 86% (CI 61.51–95%) at day 07 and 90% (CI 74.18–95%) at day 14 by WAAVP guidelines. This study represents the first documented case of ineffectiveness of anthelmintic treatment resulting in anthelmintic resistance in the central zoo. Thus, there is a requirement for a suitable and efficacious anthelmintic program.
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References
Atanaskova, E., Z. Kochevski, J. Stefanovska & G. Nikolovski (2011).
Endoparasites in wild animals at the zoological garden in Skopje,
Macedonia. Journal of Threatened Taxa 3(7): 1955–1958. https://doi.org/10.11609/jott.o2440.1955-8
Aviruppola, A.J.M.K., R.P.V.J. Rajapakse & R.S. Rajakaruna (2016).
Coprological survey of gastrointestinal parasites of mammals in
Dehiwala National Zoological Gardens, Sri Lanka. Ceylon Journal of
Science 45(1): 83. https://doi.org/10.4038/cjs.v45i1.7367
Borghare, A.T., V.P Bagde, A.D. Jaulkar, D.D. Katre, P.D. Jumde,
D.K. Mask & G.N. Bhangale (2009). Incidence of gasrointestinal
helminthiasis in captive deers at Nagpur. Veterinary World 2(9):
–338.
Cabaret, J. & B. Berrag (2004). Faecal egg count reduction test for
assessing anthelmintic efficacy: Average versus individually based
estimations. Veterinary Parasitology 121(1–2): 105–113. https://
doi.org/10.1016/j.vetpar.2004.01.020
Coles, G.C., C. Bauer, F.H.M. Borgsteede, S. Geerts, T.R. Klei, M.A.
Taylor & P.J. Waller (1992). World Association for the Advancement
of Veterinary Parasitology (W.A.A.V.P.) methods for the detection
of anthelmintic resistance in nematodes of veterinary importance.
Veterinary Parasitology 44: 35–44. https://doi.org/10.1016/0304-
(92)90141-u
Goossens, E., P. Dorny, J. Boomker, F. Vercammen & J. Vercruysse
(2005). A 12-month survey of the gastro-intestinal helminths
of antelopes, gazelles and giraffids kept at two zoos in Belgium.
Veterinary Parasitology 127(3–4): 303–312. https://doi.
org/10.1016/j.vetpar.2004.10.013
Goossens, E., J. Vercruysse, F. Vercammen & P. Dorny (2006).
Evaluation of three strategic parasite control programs in captive
wild ruminants. Journal of Zoo and Wildlife Medicine 37(1): 20–26.
https://doi.org/10.1638/05-041.1
Isaza, R., C.H. Courtney & G.V. Kollias (1990). Survey of parasite
control programs used in captive wild ruminants. Zoo Biology 9(5):
–392. https://doi.org/10.1002/zoo.1430090506
Kaplan, R.M. (2004). Drug resistance in nematodes of veterinary
importance: A status report. Trends in Parasitology 20(10): 477–
https://doi.org/10.1016/j.pt.2004.08.001
Khatun, M., N. Begum, M.A. Mamun, M.A. Mondal & M. Azam
(2014). View of Coprological study of gastrointestinal parasites
of captive animals at Rangpur Recreational Garden and Zoo in
Bangladesh. Journal of Threatened Taxa 6(8): 6142–6147. https://
doi.org/10.11609/JoTT.o3093.6142-7
Kolapo, T.U. & H.O. Jegede (2017). A Survey of Gastrointestinal
Parasites of Captive Animals at the University of Ilorin Zoological
Garden. Vom Journal of Veterinary Science 12: 17–27.
Kvapil, P., M. Kastelic, A. Dovc, E. Bártová, P. Cížek, N. Lima & Š.
Štrus (2017). An eight-year survey of the intestinal parasites of
carnivores, hoofed mammals, primates, ratites and reptiles in the
Ljubljana Zoo in Slovenia. Folia Parasitologica 64: 1–6. https://doi.
org/10.14411/fp.2017.013
Lim, Y.A.L., R. Ngui, J. Shukri, M. Rohela & H.R.M. Naim (2008).
Intestinal parasites in various animals at a zoo in Malaysia. Veterinary
Parasitology 157(1–2): 154–159. https://doi.org/10.1016/j.
vetpar.2008.07.015
McKenna, P.B. (1994). Criteria for diagnosing anthelmintic resistance
by the faecal egg count reduction test. New Zealand Veterinary
Journal 42(4): 153–154. https://doi.org/10.1080/00480169.1994.3
Mir, A.Q., K. Dua, L.D. Singla, S. Sharma & M.P. Singh (2016).
Prevalence of parasitic infection in captive wild animals in Bir Moti
Bagh Mini Zoo (Deer Park), Patiala, Punjab. Veterinary World 9(6):
–543. https://doi.org/10.14202/vetworld.2016.540-543
Nalubamba, K.S. & N.B. Mudenda (2012). Anthelmintic efficacy in
captive wild impala antelope (Aepyceros melampus) in Lusaka,
Zambia. Veterinary Parasitology 186(3–4): 532–537. https://doi.
org/10.1016/j.vetpar.2011.11.020
Ortiz, J., M.R. Ruiz De Ybáñez, T. Abaigar, M. Garijo, G. Espeso & M.
Cano (2001). Oral administration of mebendazole failed to reduce
nematode egg shedding in captive African gazelles. Onderstepoort
Journal of Veterinary Research 68(1): 79–82.
Pawar, P.D., M.W. Khasnis, N.K. Nighot, A.J.A.Y. Deshmukh, V.G.
Nimbalkar & L.D. Singla (2020). Parasitological surveillance and
successful treatment of gastrointestinal parasites of captive wild
animals with albendazole in a zoological collection. Indian Journal
of Animal Research 54(7): 895–899. https://doi.org/10.18805/
ijar.B-3842
Pun, T. (2014). Prevalence of Gastro- intestinal parasite in ruminants at
Central Zoo, Kathmandu, Nepal. Master Thesis. Central Department
of Zoology, Tribhuvan University, 44 pp.
Singh, P., M.P. Gupta, L.D. Singla, S. Sharma, B.S. Sandhu & D.R.
Sharma (2006). Parasitic infections in wild herbivores in the
Mahendra Choudhury Zoological Park, Chhatbir, Punjab. Zoo’’Print
Journal 21(11): 2459–2461. https://doi.org/10.11609/jott.
zpj.1519.2459-61
Soulsby, E.J. (2005). Helminths, Arthopods and Protozoa of
Domesticated Animals. Bailliere Tindall, London, 809pp.
Storey, B. (2015). Fecal Egg Counts: Uses and Limitations. University of
Georgia, College of Veterinary medicine, Athena, 9 pp.
Thawait, V.K., S.K. Maiti & A.A. Dixit (2014). Prevalence of gastrointestinal
parasites in captive wild animals of Nandan Van Zoo,
Raipur, Chhattisgarh. Veterinary World 7(7): 448–451. https://doi.
org/10.14202/vetworld.2014.448-451
Walker, J.G. & E.R. Morgan (2014). Generalists at the interface:
Nematode transmission between wild and domestic ungulates.
International Journal for Parasitology: Parasites and Wildlife 3(3):
–250. https://doi.org/10.1016/j.ijppaw.2014.08.001
Young, K.E., J.M. Jensen & T.M. Craig (2000). Evaluation of
anthelmintic activity in captive wild ruminants by fecal egg
reduction tests and a larval development assay. Journal of Zoo and
Wildlife Medicine 31(3): 348–352. https://doi.org/10.1638/1042-
(2000)031[0348:EOAAIC]2.0.CO;2
Zajac, A.M. & G.A. Conboy (2012). Veterinary Clinical Parasitology, 8th
Edition. Wiley Blackwell, West Sussex, UK, 354 pp.