Effectivness of the GnRH analogue deslorelin as a reversible contraceptive in a neotropical primate, the Common Marmoset Callithrix Jacchus (Mammalia: Primates: Callitrichidae)

Main Article Content

Derek A. Rosenfield
http://orcid.org/0000-0001-5546-8872
P. Viau
http://orcid.org/0000-0002-5138-3676
C. A. Oliveira
http://orcid.org/0000-0002-4143-7212
Cristiane Schilbach Pizzutto
http://orcid.org/0000-0002-4357-5133

Abstract

Deslorelin is a synthetic GnRH analogue, which is being used as a contraceptive in animals by acting as a gonadal suppressant.  The product Suprelorin (Virbac, Australia) contains deslorelin as a biocompatible, slow release subcutaneous implant. The continuous release of deslorelin provokes a down-regulation of GnRH receptors, and subsequently, inhibition of the synthesis and release of the gonadotropins FSH and LH, necessary for gonadal activities.  The intention of this study was to investigate the effectiveness of a subcutaneous deslorelin acetate implant (2,35mg) in suppressing ovarian cyclic activity and inhibiting ovulation in captive Common Marmoset Callithrix jacchus, and investigate the reversibility of the treatment.  Two experimental groups were formed, group deslorelin (D) with three couples and control group (C) with two couples.  To monitor the effect of the implants, hormones indicating ovarian cyclic activity were monitored non-invasively by enzyme immunoassay (fecal monoclonal antibody anti-progesterone CL 425).  Fecal samples were collected three times a week from all females during three trial phases (phase I: month 1,2,3 and 4; phase II: month: 5,6 and 7 and phase III: month 8,9 and 10).  In contrast to expectations the results of this trial indicated that there was no suppression of the ovarian cyclic activity, nor inhibition of the ovulation after the application of the implants.  The outcome of our trial can possibly be explained by the fact that the dosage of 2.35mg of deslorelin is not effective in C. jacchus.  We confirmed significant changes (p<0.05) of P4 metabolites from phase I to phase II due to the treatment after the implantation of the GnRH analogue Deslorelin.  The employed non-invasive fecal progesterone monitoring could be biologically validated and proved to be efficient in the detection of ovarian cyclic activity in this neotropical primate species, C. jacchus.

 

Article Details

How to Cite
[1]
Rosenfield, D.A., Viau, P., Oliveira, C.A. and Pizzutto, C.S. 2016. Effectivness of the GnRH analogue deslorelin as a reversible contraceptive in a neotropical primate, the Common Marmoset Callithrix Jacchus (Mammalia: Primates: Callitrichidae). Journal of Threatened Taxa. 8, 4 (Apr. 2016), 8652–8658. DOI:https://doi.org/10.11609/jott.2305.8.4.8652-8658.
Section
Communications
Author Biographies

Derek A. Rosenfield, University of Sao Paulo, School of Veterinary Medicine and Animal Science, Department of Animal Reproduction (VRA), Brazil

D.V.M., M.Sc., and D.Sc.-Student. Postgraduate Researcher in Wildlife Reproduction, Department of Animal Reproduction, Faculty of Veterinary Medicine and Animal Science at the University of Sao Paulo, with focus on Wildlife population control, employing non-lethal and reversible contraceptive methods.

P. Viau, University of Sao Paulo, School of Veterinary Medicine and Animal Science, Department of Animal Reproduction (VRA), Brazil

Bachelor's at Medicina Veterinária from Universidade Federal Rural de Pernambuco (2000), master's at Medicina veterinária from Faculdade de Medicina Veterinária e Zootecnia- Universidade de São Paulo (2003) and doctorate at Medicina Veterinária from FMVZ (2007).

C. A. Oliveira, University of Sao Paulo, School of Veterinary Medicine and Animal Science, Department of Animal Reproduction (VRA), Brazil

Graduate at Medicina Veterinária from Universidade de São Paulo (1981), master's at Veterinary Medicine from Universidade de São Paulo (1985) and ph.d. at Veterinary Medicine from Universidade de São Paulo (1993).

Cristiane Schilbach Pizzutto, University of Sao Paulo, School of Veterinary Medicine and Animal Science, Department of Animal Reproduction (VRA), Brazil

Veterinarian, and post doctorate from the Faculty of Veterinary Medicine and Animal Science, University of São Paulo, currently working with animal welfare and environmental enrichment; she is regional coordinator of The Shape of Enrichment (Shape Brazil).

References

Bertschinger, H.J., M.A.V.B. Guimarães, T.E. Trigg & A. Human (2008). The use of deslorelin implants for the long-term contraception of lionesses and tigers. Wildlife Research 35(6): 525–530; http://dx.doi.org/doi.org/10.1071/WR07141

D’Occhioa, M.J., Fordyceb, G., Whytea, T.R., Aspdena, W.J., Trigg, T.E. (2000). Reproductive responses of cattle to GnRH agonists. Animal Reproductive Science (60–61): 433–442; http://dx.doi.org/10.1016/S0378-4320(00)00078-6

European Association of Zoos and Aquariums (2010). Best Practice Guidelines Callithrichidae. http://www.eaza.net/assets/Uploads/CCC/2015-Callitrichids-EAZA. Accessed on 09/2015.

Flaiban, K.K.M.C., K.A.H. Spohr, L.S. Malanski, W.K. Svoboda, M.M. Shiozawa & C.L.S. Hilst (2009). Hematologic values of free-ranging Black-and-Gold Howlers Monkeys (Alouatta caraya) from the region of Upper Paraná River, southern Brazil. Arquivo Brasileiro de Medicina Veterinária e Zootecnia 61(3); http://dx.doi.org/10.1590/S0102-09352009000300016

Gobello, C. (2007). New GnRH analogs in canine reproduction. Animal Reproduction Science 100(1&2): 1–13; http://dx.doi.org/10.1016/j.anireprosci.2006.08.024

Herbet, C.A., T.E. Trigg & D.W. Cooper (2004) Effect of deslorelin implants on follicular development, parturition and post-partum oestrus in the Tammar Wallaby (Macropus eugenii). Reproduction 127: 265–273; http://dx.doi.org/10.1530/rep.1.00094

Herbet, C.A., Trigg, T.E, Cooper, D.W. (2007) Chronic Treatment of Male Tammar Wallabies with Deslorelin Implants During Pouch Life: Effects on Development, Puberty, and Reproduction in Adulthood. Biology of Reproduction 76(6): 1054–1061; http://dx.doi.org/10.1095/​biolreprod.107.059915

Kauffold J., H. Rohrmann & J. Boehm (2010). Effects of long-term treatment with the GnRH agonist deslorelin (Suprelorin®) on sexual function in boars. Theriogenology 74: 733–740; http://dx.doi.org/10.1016/j.theriogenology.2010.03.026

Kutzler, M. & A. Wood (2006). Non-surgical methods of contraception and sterilization. Theriogenology 66(3): 514–516; http://dx.doi.org/10.1016/j.theriogenology.2006.04.014

McClintock, M.K. (1983) Synchronizing Ovarian and Birth Cycles by Female Pheromones’, pp. 159–78. In: Dietland Müller-Schwarze, D. & R.M. Silverstein (eds.). Chemical Signals in Vertebrates 3 Edition. Springer US; http://dx.doi.org/10.1007/978-1-4757-9652-0_10

Mittermeier, R.A., A.B. Rylands & A.F. Coimbra-Filho (1988). Systematics: species and subspecies - an update; pp. 13–75. In: Mittermeier, R.A., A.B. Rylands, A.F. Coimbra-Filho & G.A.B. da Fonseca (eds.). Ecology and Behavior of Neotropical Primates - Vol. 2. World Wildlife Foundation, Washington D.C.; http://dx.doi.org/10.1007/978-1-4419-0293-1_2

Modesto, Thiago C., Bergallo, Helena G. (2008). Ambientes diferentes, diferentes gastos do tempo entre atividades: o caso de dois grupos mistos do exótico Callithrix spp. na Ilha Grande, RJ, Brasil. Neotropical Biology and Conservation 3(3): 112–118; http://dx.doi.org/10.4013/nbc.20083.02

Munro, C.J., G.H. Stabenfeldt, J.R Cragun, L.A. Addiego, J.W. Overstreet & B.L. Lasley (1991). Relationship of serum estradiol and progesterone concentrations to the excretion profiles of their major urinary etabolites as measured by enzyme immunoassay and radioimmunoassay. Clinical Chemistry 37(6): 838.

Munson, L., C.S. Bauman, C.S. Asa, W. Jochle & T.E. Trigg (2001). Efficacy of the GnRH analogue deslorelin for suppression of oestrous cycles in cats. Journal of Reproductive Fertility Supplement 57: 269–273.

Ortmann, O., J.M. Weiss & K. Diedrich (2002). Gonadotrophin-releasing hormone (GnRH) and GnRH agonists: mechanisms of action. Reproductive BioMedicine 5(Supplement 1): 1–7; http://dx.doi.org/10.1016/S1472-6483(11)60210-1

Padula, A.M. (2005). GnRH analogues-agonists and antagonists. Animal Reproduction Science 88(1–2): 115–126; http://dx.doi.org/10.1016/j.anireprosci.2005.05.005

Munro, C. & G. Stabenfeldt (1984). Development of a micro-titer plate enzyme immunoassay for the determination of progesterone. Journal of Endocrinology 101: 41–49; http://dx.doi.org/10.1677/joe.0.1010041

Pizzutto, C., M.G.F.G. Sgai, C.P. Francischini, P. Viau, C.A. Oliveira & M.A.B.V. Guimarães (2015). Physiological validation of enzyme immunoassay of fecal glucocorticoid metabolite levels and diurnal variation measured in captive Black-tufted Marmoset Callithrix penicillata (Mammalia: Primates: Callitrichidae). Journal of Threatened Taxa 7(6): 7234–7242; http://dx.doi.org/10.11609/JoTT.o4099.7234-42

Richardson, D.M., P. Pyšek, M. Rejmánek, M.G. Barbour, F.D. Panetta & C.J. West (2000). Naturalization and invasion of alien plants: concepts and definitions. Diversity and Distributions 6: 93–107; http://dx.doi.org/10.1046/j.1472-4642.2000.00083.x

Saltzman, W., B.K. Hogan, B.M. Horman & D.H. Abbott (2006). Social suppression of cortisol in female Marmosets: Role of luteinizing hormone/chorionic gonadotropin. General and Comparative Endocrinology 149(2006): 90–99; http://dx.doi.org/10.1016/j.ygcen.2006.05.007

St. Luis Zoo Contraceptive Center (2015). New World Monkeys-Primates; General Recommendations; GnRH Agonists. Accessed on 09/2015

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