Comparing three sampling techniques for surveying and monitoring arthropods in Moroccan agroecosystems
DOI:
https://doi.org/10.11609/jott.9639.18.2.28296-28306Keywords:
Arthropod abundance, arthropod diversity, ground beetles, flying insects, insect sampling, Morocco, mowing vegetation, pitfall traps, sight hunting, sustainable crop managementAbstract
Insect monitoring is a key component of sustainable and productive crop management. Among the various methods used to observe insect communities, pitfall trapping, visual searching, and sweep-net sampling of vegetation are the most widely applied. Selecting an appropriate sampling method is essential to obtain a comprehensive and accurate representation of species diversity. However, there is a notable lack of quantitative studies comparing the relative effectiveness of these techniques across different insect taxa In Morocco.In this study, the efficiency of three sampling strategies—pitfall trapping, mowing (sweep sampling of herbaceous plants), and visual searching—was evaluated to assess arthropod abundance and diversity in agroecosystems of northwestern Morocco. Between spring and summer 2020, a total of 69 species belonging to seven orders and 27 families were recorded. Pitfall traps and visual searching proved most effective for capturing ground beetles, whereas mowing herbaceous vegetation was particularly effective for collecting flying insects.These results highlight the importance of carefully selecting sampling techniques to ensure accurate estimates of arthropod diversity and abundance. Combining multiple methods provides a more comprehensive overview of arthropod communities in any ecosystem, including agroecosystems.
References
Biesmeijer, J.C., S.P.M. Roberts, M. Reemer, R. Ohlemüller, M. Edwards, T. Peeters, A.P. Schaffers, S.G. Potts, R. Kleukers, C.D. Thomas, J. Settele & W.E. Kunin (2006). Parallel declines in pollinators and insect-pollinated plants in Britain and the Netherlands. Science 313(5785): 351–354. https://doi.org/10.1126/science.1127863
Bouget, C., L.M. Nageleisen & C. Bouget (2009). Les groupes d’insectes cibles en forêt tempérée: chapter 4, part I Les coléoptères carabidae. L’étude des insectes en forêt: méthodes et techniques, éléments essentiels pour une standardisation. Les dossiers forestiers 19: 91–98.
Buchholz, S. & K. Hannig (2013). Do covers influence the capture efficiency of pitfall traps. European Journal of Entomology 106: 667–671. https://doi.org/10.14411/eje.2009.083
Carvalheiro, L.G., C.L. Seymour, R. Veldtman & S.W. Nicolson (2010). Pollination services decline with distance from natural habitat even in biodiversity-rich areas. Journal of Applied Ecology 47(4): 810–820. https://doi.org/10.1111/j.1365-2664.2010.01829.x
Colinet, H., B.J. Sinclair, P. Vernon & D. Renault (2015). Insects in fluctuating thermal environments. Annual Review of Entomology 60: 123–140. https://doi.org/10.1146/annurev-ento-010814-021017
Corti, R., S.T. Larned & T. Datry (2013). A comparison of pitfall-trap and quadrat methods for sampling ground-dwelling invertebrates in dry riverbeds. Hydrobiologia 717: 13–26. https://doi.org/10.1007/s10750-013-1563-0
Coscarón, M.D.C., M.C. Melo, J. Coddington & J. Corronca (2009). Estimating biodiversity: a case study on true bugs in Argentinian wetlands. Biodiversity and Conservation 18: 1491–1507.
Dirzo, R., H.S. Young, M. Galetti, G. Ceballos, N.J.B. Isaac & B. Collen (2014). Defaunation in the Anthropocene. Science 345(6195): 401–406. https://doi.org/10.1126/science.1251817
El Harche, H., G. Chavanon, J. Dahmani & M. Fadli (2022). Checklist of terrestrial beetles (Arthropoda: Insecta: Coleoptera) associated with agroecosystems in North-West Morocco. Arxius de Miscel·lània Zoològica 20: 59–81. https://doi.org/10.32800/amz.2022.20.0059
El Harche, H., S. El Hassouni, M. Fadli & J. Dahmani (2023). Spatial, seasonal variation and impacts of anthropogenic factors on insect assemblages (Arthropoda: Insecta) in northwest Morocco. Biodiversitas 24(10): 5368–5375. https://doi.org/10.13057/biodiv/d241019
Forbes, A.A., R.K. Bagley, M.A. Beer, A.C. Hippee & H.A. Widmayer (2018). Quantifying the unquantifiable: why Hymenoptera, not Coleoptera, is the most speciose animal order. BMC Ecology 18: 1–11. https://doi.org/10.1186/s12898-018-0176-x
Ganaoui, N., M.C. Maazi & A. Chefrour (2019). Spatio-temporal variation of scarab beetles (Coleoptera: Scarabaeidae) in two oak biotopes of Ouled Bechih Forest, Souk-Ahras region (north-eastern Algeria). Polish Journal of Entomology 88(4): 301–319.
Galloway, A.D., C.L. Seymour, R. Gaigher & J.S. Pryke (2021). Organic farming promotes arthropod predators, but this depends on neighbouring patches of natural vegetation. Agriculture, Ecosystems & Environment 310: 107295. https://doi.org/10.1016/j.agee.2020.107295
García-López, A., E. Micó, M.A. Zumbado & E. Galante (2011). Sampling Scarab Beetles in Tropical Forests: The Effect of Light Source and Night Sampling Periods. Journal of Insect Science 11(95): 1–14. https://doi.org/10.1673/031.011.9501
Gardarin, A., M. Plantegenest, A. Bischoff & M. Valantin-Morison (2018). Understanding plant–arthropod interactions in multitrophic communities to improve conservation biological control: useful traits and metrics. Journal of Pest Science 91(3): 943–955. https://doi.org/10.1007/s10340-018-0958-0
Haavik, L.J. & F.M. Stephen (2023). Insect Ecology, pp. 91–114. In: Allison, D.J., T.D. Paine, B. Slippers & M.J. Wingfield (eds.). Forest Entomology and Pathology. Springer, Cham., 789 pp. https://doi.org/10.1007/978-3-031-11553-0
Hohbein, R.R. & C.J. Conway (2018). Pitfall traps: a review of methods for estimating arthropod abundance. Wildlife Society Bulletin 42: 597–606.
Landis, D.A., S.D. Wratten & G.M. Gurr (2000). Habitat management to conserve natural enemies of arthropod pests. Annual Review of Entomology 45: 175–201. https://doi.org/10.1146/annurev.ento.45.1.175
Makwela, M.M., R. Slotow & T.C. Munyai (2025). Ecological response of carabid beetles (Coleoptera: Carabidae) to contrasting agroecosystem management. Community Ecology 26: 705–716. https://link.springer.com/article/10.1007/s42974-025-00261-6
Morshed, M.N., M.A. Al Mamun, S.A.I. Nihad, M.M. Rahman, N. Sultana & M.M. Rahman (2023). Effect of weather variables on seasonal abundance of rice insects in southeast coastal region of Bangladesh. Journal of Agriculture and Food Research 11: 100513. https://doi.org/10.1016/j.jafr.2023.100513
Potts, S.G., J.C. Biesmeijer, C. Kremen, P. Neumann, O. Schweiger & W.E. Kunin (2010). Global pollinator declines: trends, impacts and drivers. Trends in Ecology & Evolution 25(6): 345–353. https://doi.org/10.1016/j.tree.2010.01.007
Pizzolotto, R., A. Mazzei, T. Bonacci, S. Scalercio, N. Iannotta & P. Brandmayr (2018). Ground beetles in Mediterranean olive agroecosystems: Their significance and functional role as bioindicators (Coleoptera, Carabidae). PLoS ONE 13(3): e0194551. https://doi.org/10.1371/journal.pone.0194551
Quinto, J., M. de los Á. Marcos-García, H. Brustel, E. Galante & E. Micó (2013). Effectiveness of three sampling methods to survey saproxylic beetle assemblages in Mediterranean woodland. Journal of Insect Conservation 17(4): 765–776. https://doi.org/10.1007/s10841-013-9559-7
Robinson, S.I., Ó.B. McLaughlin, B. Marteinsdóttir & E.J. O’Gorman (2018). Soil temperature effects on the structure and diversity of plant and invertebrate communities in a natural warming experiment. Journal of Animal Ecology 87: 634–646. https://doi.org/10.1111/1365-2656.12798
Russo, L., R. Stehouwer, J.M. Heberling & K. Shea (2011). The composite insect trap: an innovative combination trap for biologically diverse sampling. PLoS ONE 6(6): e21079. https://doi.org/10.1371/journal.pone.0021079
Sabu, T.K., R.T. Shiju, K. Vinod & S. Nithya (2011). A comparison of the pitfall trap, Winkler extractor and Berlese funnel for sampling ground-dwelling arthropods in tropical montane cloud forests. Journal of Insect Science 11: 1–19. https://doi.org/10.1673/031.011.0128
Sentil, A., P. Rosa, C. Tourbez, A. Dorchin, P. Bogusch & D. Michez (2024). New records of bees (Hymenoptera: Apoidea) from Morocco. Journal of Hymenoptera Research 97: 513–530. https://doi.org/10.3897/jhr.97.125408
Skvarla, M., J. Larson & A. Dowling (2014). Pitfalls and preservatives: a review. The Journal of the Entomological Society of Ontario 145: 15–43.
Sial, M.U., M.Z. Majeed, A. Atiq, T. Farooq, H.M. Aatif, W. Jaleel & H.A. Ghrmah (2022). Differential efficacy of edaphic traps for monitoring arthropods diversity in subtropical regions. Journal of King Saud University-Science 34(1): 101686. https://doi.org/10.1016/j.jksus.2021.101686
Taraborelli, P., N. Carrasco, M. Malaspina, M. Domínguez, A. Belaus, A. López, A. Scavone, A. Barbera & M. Zamora (2022). Evaluation of agrobiodiversity and its trophic interactions as an indicator of sustainability in productive systems. Open Journal of Environmental Biology 7(1): 006–013. https://doi.org/10.17352/ojeb.000027
Vasconcelos, C., M. Dittrich & J.A. McKenzie (2014). Evidence of microbiocoenosis in the formation of laminae in modern stromatolites. Facies 60: 3–13. https://doi.org/10.1007/s10347-013-0371-3
Von Hoermann, C., D. Jauch, C. Kubotsch, K. Reichel–Jung, S. Steiger & M. Ayasse (2018). Effects of abiotic environmental factors and land use on the diversity of carrion–visiting silphid beetles (Coleoptera: Silphidae): A large-scale carrion study. PLoS ONE 13(5): e0196839. https://doi.org/10.1371/journal.pone.0196839
Zhao, L., R. Gao, J. Liu, L. Liu, R. Li, L. Men & Z. Zhang (2023). Effects of Environmental Factors on the Spatial Distribution Pattern and Diversity of Insect Communities along Altitude Gradients in Guandi Mountain, China. Insects 14(3): 224. https://doi.org/10.3390/insects14030224
Zaller, J.G., G. Kerschbaumer, R. Rizzoli, A. Tiefenbacher, E. Gruber & H. Schedl (2015). Monitoring arthropods in protected grasslands: comparing pitfall trapping, quadrat sampling and video monitoring. Web Ecology 15(1): 15–23. https://doi.org/10.5194/we-15-15
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