Taxonomic studies and breaking seed dormancy of Hibiscus lobatus (Murray) Kuntze, 1898 (Magnoliopsida: Malvales: Malvaceae) — a native plant of the central Western Ghats
DOI:
https://doi.org/10.11609/jott.9610.17.8.27323-27332Keywords:
Annual herb, ethnobotanical survey, Gibberellic acid (GA₃), native flora conservation, propagation techniques, scarification, seasonal germination, seed viability, tetrazolium test, Western Ghats biodiversityAbstract
Hibiscus lobatus (Murray) Kuntze is a medicinal plant of the Malvaceae family, very sparsely and seasonally distributed, particularly in the Western Ghats of Karnataka. This species, known for its ethnopharmacological uses, especially in wound healing, faces a declining population due to habitat loss, overexploitation, and poor seed germination. Seed dormancy has been identified as a major factor limiting its natural regeneration. In this study, the distribution, habitat, and taxonomic diagnostic features of Hibiscus lobatus were investigated across different floristic regions of the central Western Ghats. Seed viability and dormancy-breaking treatments were assessed both in the field and in vitro. Tetrazolium testing confirmed seed viability, but field germination rates were low (4–6%), indicating strong dormancy. Among the treatments tested, sulfuric acid scarification combined with GA₃ application proved most effective, substantially enhancing germination and reducing germination time. These findings establish an optimised protocol for overcoming seed dormancy in Hibiscus lobatus, which can be applied to conserve and propagate this important medicinal species, addressing its declining population.
References
Anjali, A.K.K., S. Agarwal, C. Pandey & V. Gupta (2015). Responses to seed dormancy breaking treatments in wild Hibiscus species. Vegetos 28(2): 143.
Bachman, S.P., E.M.N. Lughadha & M.C. Rivers (2018). Quantifying progress toward a conservation assessment for all plants. Conservation Biology 32(3): 516–524. https://doi.org/10.1111/cobi.13071
Benjamin, J.F. & G.V.S. Murthy (2013). Flora of Sri Venkateswara National Park, Andhra Pradesh. Botanical Survey of India, Kolkata, 492 pp.
Benedito, C.P., M.C.C. Ribeiro, E.P.D. Paiva & H.L.D.S. Medeiros (2019). Dormancy overcoming and germination test in Piptadenia stipulacea (Benth.) Ducke seeds. Revista Ciência Agronômica 50(2): 338–344. https://doi.org/10.5935/1806-6690.20190040
Bridson, D. & L. Forman (1992). The Herbarium Handbook. 3rd edition. Royal Botanic Gardens, Kew, England.
Davies, P.J. (ed.) (1995). Plant Hormones: Physiology, Biochemistry and Molecular Biology. Kluwer Academic (Springer Science & Business Media), Dordrecht, 833 pp.
di Martino, O., A. Tito, A. de Lucia, A. Cimmino, F. Cicotti, F. Apone & V. Calabrò (2017). Hibiscus syriacus extract from an established cell culture stimulates skin wound healing. BioMed Research International 2017: 7932019. https://doi.org/10.1155/2017/7932019
Dilaver, Z., M. Mirzapour & H. Kendir (2017). Breaking seed dormancy and micropropagation of perennial vulneraria milkvetch (Astragalus vulnerariae DC.). Acta Scientiarum Polonorum. Hortorum Cultus 16(4): https://doi.org/10.24326/asphc.2017.4.9
Donohue, K, R.R. de Casas, L. Burghardt, K. Kovach & C.G. Willis (2010). Germination, post-germination adaptation, and species ecological ranges. Annual Review of Ecology, Evolution, and Systematics 41: 293–319. https://doi.org/10.1146/annurev-ecolsys-102209-144715
Dos, S.N.L.B., A. Gori, A. Raffaelli, F. Ferrini & C. Brunetti (2021). Phenolic compounds from leaves and flowers of Hibiscus roseus: Potential skin cosmetic applications of an under-investigated species. Plants 10(3): 522. https://doi.org/10.3390/plants10030522
Du Plessis, H.J., R. Kleynhans, R.V. Nikolova & B.A. Egan (2019). Factors affecting seed propagation of Hibiscus coddii subsp. barnardii: a new potential ornamental plant. Propagation of Ornamental Plants 19(2): 38–47.
Bhat, K.G. (2014). Flora of South Kanara: Dakshina Kannada and Udupi Districts of Karnataka. Taxonomy Research Centre, 251 pp.
Hamid, M., A.A. Khuroo, A.H. Malik, R. Ahmad, C.P. Singh, J. Dolezal & S.M. Haq (2020). Early evidence of shifts in alpine summit vegetation: a case study from Kashmir Himalaya. Frontiers in Plant Science 11: 421. https://doi.org/10.3389/fpls.2020.00421
Harel, D., C. Holzapfel & M. Sternberg (2011). Seed mass and dormancy of annual plant populations and communities decreases with aridity and rainfall predictability. Basic and Applied Ecology 12(8): 674–684. https://doi.org/10.1016/j.baae.2011.09.003
Henry, A.N. (1983). Flora of Tamil Nadu, India. Series I: Analysis, Vol. 1. Botanical Survey of India, Coimbatore, 38–42 pp.
Hooker, J.D. (1875). The Flora of British India: Ranunculaceae to Sapindaceae, Vol. 1. Reeve & Co., London, 317–353 pp.
India Biodiversity Portal (2025). Hibiscus lobatus (Murray) Kuntze. https://indiabiodiversity.org/observat ion/show/19088124. Accessed on 11.viii.2025.
Leubner-Metzger, G. (2006) Seed dormancy and the control of germination. New Phytologist 171: 501–523. https://doi.org/10.1111/j.1469-8137.2006.01787.x
Li, J., Y.R. Lu, I.F. Lin, W. Kang, H.B. Chen, H.F. Lu & H.M.D. Wang (2020) Reversing UVB-induced photoaging with Hibiscus sabdariffa calyx aqueous extract. Journal of the Science of Food and Agriculture 100(2): 672–681. https://doi.org/10.1002/jsfa.10063
Luan, F., Q. Wu, Y. Yang, H. Lv, D. Liu, Z. Gan & N. Zeng (2020). Traditional uses, chemical constituents, biological properties, clinical settings, and toxicities of Abelmoschus manihot L.: a comprehensive review. Frontiers in Pharmacology 11: 1068. https://doi.org/10.3389/fphar.2020.01068
Noori, S., Tabassum, H. & Inamati, S (2025). Geospatial analysis of Agroforestry landscape in the Central Western Ghats, India, using Google Earth Engine. Agroforest Systems 99(181): 3. https://doi.org/10.1007/s10457-025-01266-w
Pace, B.A., H.M. Alexander, D.J. Emry & K.L. Mercer (2016). Reliable method for assessing seed germination, dormancy, and mortality under field conditions. Journal of Visualized Experiments (117): e54663. https://doi.org/10.3791/54663
Patil, V.N. & M. Dadlani (2009). Tetrazolium test for seed viability and vigour, pp. 209–241. In: Handbook of Seed Testing. National Seeds Corporation, New Delhi, India.
Pradhan, B.K. & H.K. Badola (2011). Effects of microhabitat, light and temperature on seed germination of a critically endangered Himalayan medicinal herb, Swertia chirayita: Conservation implications. Plant Biosystems 146(2): 345–351. https://doi.org/10.1080/11263504.2011.620641
Ramachandra, T.V., A.H. Srijith & S. Bharath (2019). Land surface temperature responses to the land cover dynamics in Western Ghats. Sahyadri E-news 66: 3–66. https://wgbis.ces.iisc.ac.in/biodiversity/sahyadri_enews/newsletter/Issue66/biblio.html Accessed on 12.viii.2025.
Ramaswamy, S.N., R.K. Rao & G.D. Arekal (2001). Flora of Shimoga District. Prasaranga, University of Mysore, Mysore, 78–87 pp.
Saldanha, C.J. (1984). Flora of Karnataka, Vol. 1. Oxford & IBH Publishing Co., New Delhi, 250 pp.
Sankararao, K., R.K. Swamy, D. Kumar, R.A. Singh & K.G. Bhat (2019). Flora of Peninsular India. https://indiaflora-ces.iisc.ac.in/FloraPeninsular/herbsheet.php?id=5628&cat=7
Tali, B.A., A.H. Ganie, I.A. Nawchoo, A.A. Wani & Z.A. Reshi (2015). Assessment of threat status of selected endemic medicinal plants using IUCN regional guidelines: A case study from Kashmir Himalaya. Journal for Nature Conservation 23: 80–89. https://doi.org/10.1016/j.jnc.2014.06.004
Tiwari, R.K.S., K.K. Chandra & S. Dubey (2018). Techniques for breaking seed dormancy and its efficacy on seed germination of six important medicinal plant species. International Journal of Agriculture, Environment and Biotechnology 11(2): 293–301. http://doi.org/10.30954/0974-1712.04.2018.10
Urban, M.C. (2015). Accelerating extinction risk from climate change. Science 348(6234): 571–573. https://doi.org/10.1126/science.aaa4984
WFO (2025). Hibiscus lobatus (Murray) Kuntze. Available at: http://www.worldfloraonline.org/taxon/wfo-0000722748. Accessed on 12.viii.2025.
Wolkis, D. & S.K. Walsh (2018). Dormancy and germination of two Kaua‘i endemic Hibiscus taxa. Seed Science and Technology 46(2): 267–274. https://doi.org/10.15258/sst.2018.46.2.08
Yang, J.E., H.T. Ngo, E. Hwang, S.A. Seo, S.W. Park & T.H. Yi (2019). Dietary enzyme-treated Hibiscus syriacus L. protects skin against chronic UVB-induced photoaging via enhancement of skin hydration and collagen synthesis. Archives of Biochemistry and Biophysics 662: 190–200. https://doi.org/10.1016/j.abb.2018.12.020
Zhang, W., L.W. Qu, J. Zhao L. Xue, H.P. Dai, G.M. Xing & J.J. Lei (2020). Practical methods for breaking seed dormancy in a wild ornamental tulip species Tulipa thianschanica Regel. Agronomy 10(11): 1765. https://doi.org/10.3390/agronomy10111765
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