Assessment of the status of Spodoptera species (Lepidoptera: Noctuidae: Armyworm) in India through DNA barcoding technique
DOI:
https://doi.org/10.11609/jott.8983.16.7.25528-25535Keywords:
Biodiversity in India, conservation, current status, insect, identification, molecular biology, species, taxaAbstract
Insects constitute the majority of animal fauna worldwide, but quantifying their species diversity is still incomplete. A few recent studies indicate a marked decrease in the population of insects which calls for urgent efforts to document and understand insect diversity to get a complete picture of Earth’s ecosystems. Modern technology can accelerate species identification beyond traditional methods’ limitations. Hence, a focused and expedited approach through DNA barcoding coupled with morphological identification is necessary. This present research highlights the gaps that exist and it examines the current status of Spodoptera species barcode in India. Six Spodoptera species were studied confirming their presence in India including two invasive species. That means less than 50% of taxa or described Spodoptera species are covered by genetic data from barcoded specimens after analysis. Therefore, comprehensive DNA barcoding should be achieved from all insect species occurring on the Indian subcontinent to speed up the discovery and documentation of new species by involving both traditional taxonomists and molecular biologists working towards a common goal.
References
Benson, D.A., M. Cavanaugh, K. Clark, I. Karsch-Mizrachi, D.J. Lipman, J. Ostell & E.W. Sayers (2012). GenBank. Nucleic Acids Research 41(D1): D36–D42. https://doi.org/10.1093/nar/gks1195
Chandel, R.S., V.K. Chandla, K.S. Verma & M. Pathania (2013). Insect pests of potato in India, pp. 227–268. In: Insect Pests of Potato. Elsevier. https://doi.org/10.1016/B978-0-12-386895-4.00008-9
Dasmahapatra, K.K., M. Elias, R.I. Hill, J.I. Hoffman & J. Mallet (2010). Mitochondrial DNA barcoding detects some species that are real, and some that are not. Molecular Ecology Resources 10(2): 264–273. https://doi.org/10.1111/j.1755-0998.2009.02763.x
Dumas, P., J. Barbut, B. Le Ru, J. F.Silvain, A.L. Clamens, E. d’Alençon & G.J. Kergoat (2015). Phylogenetic molecular species delimitations unravel potential new species in the pest genus Spodoptera Guenée, 1852 (Lepidoptera, Noctuidae). Plos ONE 10(4): e0122407. https://doi.org/10.1371/journal.pone.0122407
European and Mediterranean Plant Protection Organization (OEPP/EPPO) (2015). EPPO standards PM 7/124(1) diagnostic protocol for Spodoptera littoralis, Spodoptera litura, Spodoptera frugiperda, Spodoptera eridania. EPPO Bulletin 45(3): 410–444. https://doi.org/10.1111/epp.12258
Ganiger, P.C., H.M. Yeshwanth, K. Muralimohan, N. Vinay, A.R.V. Kumar & K. Chandrashekara (2018). Occurrence of the New Invasive Pest, Fall Armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), in the Maize Fields of Karnataka, India. Current Science 115(4): 621. https://doi.org/10.18520/cs/v115/i4/621-623
Goergen, G., P.L. Kumar, S.B. Sankung, A. Togola & M. Tamò (2016). First report of outbreaks of the Fall Armyworm Spodoptera frugiperda (J E Smith) (Lepidoptera, Noctuidae), a new alien invasive pest in West and Central Africa. Plos ONE 11(10): e0165632. https://doi.org/10.1371/journal.pone.0165632
Gouin, A., A. Bretaudeau, K. Nam, S. Gimenez, J.M. Aury, B. Duvic, F. Hilliou, N. Durand, N. Montagné, I. Darboux, S. Kuwar, T. Chertemps, D. Siaussat, A. Bretschneider, Y. Moné, S.J. Ahn, S. Hänniger, A.S.G. Grenet, D. Neunemann & P. Fournier (2017). Two genomes of highly polyphagous lepidopteran pests (Spodoptera frugiperda, Noctuidae) with different host-plant ranges. Scientific Reports 7(1): 11816. https://doi.org/10.1038/s41598-017-10461-4
Hebert, P.D.N., A. Cywinska, S.L. Ball & J.R. DeWaard (2003). Biological identifications through DNA barcodes. Proceedings of the Royal Society of London. Series B: Biological Sciences 270(1512): 313–321. https://doi.org/10.1098/rspb.2002.2218
Henaish, M. & N. Elmetwaly (2020). Identification and taxonomic notes of spodoptera species (Lepidoptera: Noctuidae) known to occur in Egypt. Egyptian Academic Journal of Biological Sciences. A, Entomology 13(2): 161–175. https://doi.org/10.21608/eajbsa.2020.88035
Jing, D., J. Guo, Y. Jiang, J. Zhao, A. Sethi, K. He & Z. Wang (2020). Initial detections and spread of invasive Spodoptera frugiperda in China and comparisons with other noctuid larvae in cornfields using molecular techniques. Insect Science 27(4): 780–790. https://doi.org/10.1111/1744-7917.12700
Kapli, P., S. Lutteropp, J. Zhang, K. Kobert, P. Pavlidis, A. Stamatakis & T. Flouri (2017). Multi-rate Poisson tree processes for single-locus species delimitation under maximum likelihood and Markov chain Monte Carlo. Bioinformatics 33(11): 1630–1638. https://doi.org/10.1093/bioinformatics/btx025
Kergoat, G.J., P.Z. Goldstein, B. Le Ru, R.L. Meagher, A. Zilli, A. Mitchell, A.L. Clamens, S. Gimenez, J. Barbut, N. Nègre, E. d’Alençon & K. Nam (2021). A novel reference dated phylogeny for the genus Spodoptera Guenée (Lepidoptera: Noctuidae: Noctuinae): new insights into the evolution of a pest-rich genus. Molecular Phylogenetics and Evolution 161: 107161. https://doi.org/10.1016/j.ympev.2021.107161
Kergoat, G.J., D.P. Prowell, B.P. Le Ru, A. Mitchell, P. Dumas, A. L. Clamens, F.L. Condamine & J.F. Silvain (2012). Disentangling dispersal, vicariance and adaptive radiation patterns: A case study using armyworms in the pest genus Spodoptera (Lepidoptera: Noctuidae). Molecular Phylogenetics and Evolution 65(3): 855–870. https://doi.org/10.1016/j.ympev.2012.08.006
Khedkar, G.D., S.B. Abhayankar, D. Nalage, S.N. Ahmed & C.D. Khedkar (2016). DNA barcode based wildlife forensics for resolving the origin of claw samples using a novel primer cocktail. Mitochondrial DNA Part A 27(6): 3932–3935. https://doi.org/10.3109/19401736.2014.987270
Khilare, V., A. Tiknaik, B. Prakash, B. Ughade, G. Korhale, D. Nalage, N. Ahmed, C. Khedkar & G. Khedkar (2019). Multiple tests on saffron find new adulterant materials and reveal that Ist grade saffron is rare in the market. Food Chemistry 272: 635–642. https://doi.org/10.1016/j.foodchem.2018.08.089
Kimura, M. (1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16(2): 111–120. https://doi.org/10.1007/BF01731581
Le Ru, B., J. Barbut, C. Capdevielle-Dulac, M. Goftishu & G.J. Kergoat (2018). Re-establishment of Spodoptera teferii Laporte in Rougeot (Lepidoptera: Noctuidae, Noctuinae), with an updated molecular phylogeny for the genus Spodoptera Guenée. Annales de La Société Entomologique de France (N.S.) 54(6): 497–510. https://doi.org/10.1080/00379271.2018.1528886
Madhu, T.N., R. T.P. Pandian, S.E. Apshara, A. Bhavishya, A. Josephrajkumar, B.J.N. Kumar & P.S. Kumar (2023). New Occurrence of the Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae) Infestation on Cocoa in India. The Journal of the Lepidopterists’ Society 77(2): 110–115. https://doi.org/10.18473/lepi.77i2.a4
Mahmood-ur-Rahman, M. Qasim, S.A. Bukhari & T. Shaheen (2014). Chapter 6 -Bt Crops: a sustainable approach towards biotic stess tolerance, pp. 125–142. In: Emerging Technologies and Management of Crop Stress Tolerance. Volume-1. Elsevier, 551 pp. https://doi.org/10.1016/B978-0-12-800876-8.00006-0
Meagher, R.L., R.N. Nagoshi, C. Stuhl & E.R. Mitchell (2004). Larval development of fall armyworm (lepidoptera: noctuidae) on different cover crop plants. Florida Entomologist 87(4): 454–460. https://doi.org/10.1653/0015-4040(2004)087[0454:LDOFAL]2.0.CO;2
Muthusamy, R., G. Ramkumar, S. Kumarasamy, M.F. Albeshr, A.F. Alrefaei, Y. Ma & M. Narayanan (2024). Resistance to synthetic pyrethroid and neonicotinoid is associated with reduced reproductive efficiency in the field population of Spodoptera litura (Insecta: Lepidoptera). Biocatalysis and Agricultural Biotechnology 56: 103031. https://doi.org/10.1016/j.bcab.2024.103031
Nalage, D., T. Sontakke, R. Patil & A. Biradar (eds.) (2023). Environmental Impact Assessment. Gaurang Publishing Globalize Private Limited. Mumbai, 108 pp.
Nalage, D., T. Sontakke, R. Kale, K. Patil & V. Dange (eds.) (2023). Integrated Pest Management. Gaurang Publishing Globalize Private Limited. Mumbai, 89 pp. https://doi.org/10.5281/ZENODO.10823681
Pashley, D. P. (1986). Host-associated genetic differentiation in fall armyworm (Lepidoptera: Noctuidae): a sibling species complex? Annals of the Entomological Society of America 79(6): 898–904. https://doi.org/10.1093/aesa/79.6.898
Patil, R., R. Satpute & D. Nalage (2023). The application of omics technologies to toxicology. Toxicology Advances 5(2): 6. https://doi.org/10.53388/TA202305006
Pons, J., T.G. Barraclough, J. Gomez-Zurita, A. Cardoso, D.P. Duran, S. Hazell, S. Kamoun, W.D. Sumlin & A.P. Vogler (2006). Sequence-Based species delimitation for the DNA taxonomy of undescribed insects. Systematic Biology 55(4): 595–609. https://doi.org/10.1080/10635150600852011
Ramaiah, M., T.U. Maheswari & N. Kamakshi (2022). Biology and morphometric studies of beet armyworm, Spodoptera exigua (Hub.). Journal of Entomological Research 46(4): 883–887. https://doi.org/10.5958/0974-4576.2022.00152.9
Ratnasingham, S. & P.D.N. Hebert (2007). bold: The Barcode of Life Data System (http://www.barcodinglife.org). Molecular Ecology Notes 7(3): 355–364. https://doi.org/10.1111/j.1471-8286.2007.01678.x
Rubinoff, D., S. Cameron & K. Will (2006). A Genomic Perspective on the Shortcomings of Mitochondrial DNA for “Barcoding” Identification. Journal of Heredity 97(6): 581–594. https://doi.org/10.1093/jhered/esl036
Saitou, N. & M. Nei (1987). The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4(4): 406–425. https://doi.org/10.1093/oxfordjournals.molbev.a040454
Shashank, P.R., N.L. Naveena, N.N. Rajgopal, T.A. Elliott, K. Sreedevi, S. Sunil & N.M. Meshram (2022). DNA barcoding of insects from India: Current status and future perspectives. Molecular Biology Report 49(11): 10617–10626. https://doi.org/10.1007/s11033-022-07628-2
Silva-Brandão, K.L., M.L. Lyra & A.V.L. Freitas (2009). Barcoding lepidoptera: Current situation and perspectives on the usefulness of a contentious technique. Neotropical Entomology 38(4): 441–451. https://doi.org/10.1590/S1519-566X2009000400001
Sontakke, T., A. Biradar & D. Nalage (2023). The role of genetics in determining resistance to coccidiosis in goats a review of current research and future directions. Molecular Biology Reports 50(7): 6171–6175. https://doi.org/10.1007/s11033-023-08520-3
Suryawanshi, R., A. Tathe, M. Salunke, D. Nalage & A. Kalyankar (2020). DNA barcoding analysis of brackish water shrimps from chilika lagoon. Journal of Emerging Technologies and Innovative Research 7(10): 838–846. https://doi.org/10.5281/ZENODO.11470556
Tiknaik, A., A. Kalyankar, M. Shingare, R. Suryawanshi, B. Prakash, T. A. Sontakke, D. Nalage, R. Sanil & G. Khedkar (2019). Refutation of media reports on introduction of the red bellied piranha and potential impacts on aquatic biodiversity in India. Mitochondrial DNA Part A 30(4): 643–650. https://doi.org/10.1080/24701394.2019.1611798
Van de Vossenberg, B. T. L. H. & M. J. Van der Straten (2014). Development and validation of real-time PCR tests for the identification of four Spodoptera species: Spodoptera eridania, Spodoptera frugiperda, Spodoptera littoralis, and Spodoptera litura (Lepidoptera: Noctuidae). Journal of Economic Entomology 107(4): 1643–1654. https://doi.org/10.1603/ec14132
Yang, Z. & B. Rannala (2010). Bayesian species delimitation using multilocus sequence data. Proceedings of the National Academy of Sciences 107(20): 9264–9269. https://doi.org/10.1073/pnas.0913022107
Published
Issue
Section
License
Copyright (c) 2024 Dinesh Nalage, P.S. Kudnar, Tejswini Sontakke, Ishwar Chittapure, Yashdeep Gowda, Shantanu Kharbal, Yashashri Alamwar

This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors own the copyright to the articles published in JoTT. This is indicated explicitly in each publication. The authors grant permission to the publisher Wildlife Information Liaison Development (WILD) Society to publish the article in the Journal of Threatened Taxa. The authors recognize WILD as the original publisher, and to sell hard copies of the Journal and article to any buyer. JoTT is registered under the Creative Commons Attribution 4.0 International License (CC BY), which allows authors to retain copyright ownership. Under this license the authors allow anyone to download, cite, use the data, modify, reprint, copy and distribute provided the authors and source of publication are credited through appropriate citations (e.g., Son et al. (2016). Bats (Mammalia: Chiroptera) of the southeastern Truong Son Mountains, Quang Ngai Province, Vietnam. Journal of Threatened Taxa 8(7): 8953–8969. https://doi.org/10.11609/jott.2785.8.7.8953-8969). Users of the data do not require specific permission from the authors or the publisher.





