DNA barcoding reveals a new population of the threatened Atlantic Forest frog Sphaenorhynchus canga
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Abstract
Species identification plays a significant role in biodiversity conservation. As many species remain unrecognized, particularly in neotropical hotspots like the Brazilian Atlantic Forest (AF), novel molecular techniques are being widely employed to bridge this gap. In this study, we used DNA barcoding and phylogenetic tools to identify a new population of Sphaenorynchus canga in the central region of the Brazilian AF. Our results extend the species’ known distribution by approximately 200 km to the south, encompassing a different mountain range than its type locality (Serra do Espinhaço). This disjunct distribution, while not uncommon among amphibians, suggests a historical connection between these two mountain complexes as a biogeographic explanation. Despite the discovery of a new S. canga population, the species continues to face numerous anthropogenic threats such as mining, land use, and cattle ranching. Urgent conservation and research efforts are warranted to ensure the survival of S. canga populations across these habitats.
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References
Araujo-Vieira, K., J.V.A. Lacerda, T.L. Pezzuti, F.S.F. Leite, C.L. Assis & C.A.G. Cruz (2015). A new species of Hatchet-faced Treefrog Sphaenorhynchus Tschudi (Anura: Hylidae) from Quadrilátero Ferrífero, Minas Gerais, southeastern Brazil. Zootaxa 4059: 96–114. https://doi.org/10.11646/zootaxa.4059.1.5 DOI: https://doi.org/10.11646/zootaxa.4059.1.5
Araujo‐Vieira, K., B.L. Blotto, U. Caramaschi, C.F.B. Haddad, J. Faivovich & T. Grant (2019). A total evidence analysis of the phylogeny of hatchet‐faced treefrogs (Anura: Hylidae: Sphaenorhynchus ). Cladistics 35(5): 469–486. https://doi.org/10.1111/cla.12367 DOI: https://doi.org/10.1111/cla.12367
Assis, C.L., D.J. Santana, F.A. Silva, F.M. Quintela & R.N. Feio (2013). A new and possibly critically endangered species of casque-headed tree frog Aparasphenodon Miranda-Ribeiro, 1920 (Anura, Hylidae) from southeastern Brazil. Zootaxa 3716: 583–591. https://doi.org/10.11646/zootaxa.3716.4.6 DOI: https://doi.org/10.11646/zootaxa.3716.4.6
Baêta, D., A.C.C. Lourenço, T.L. Pezzuti & M.R.S. Pires (2007). The tadpole, advertisement call, and geographic distribution of Physalaemus maximus Feio, Pombal & Caramaschi, 1999 (Amphibia, Anura, Leiuperidae). Arquivos do Museu Nacional 65: 27–32.
Bastos, R.P., M.R. Martins, Y.S.L. Bataus, L.G. Côrtes, V.M. Uhlig, A.P.L. Almeida, C.C. Canedo, U. Caramaschi, C.O.R. Costa, L. Ferrante, T.B. Ferreira, P.C.A. Garcia, J.L. Gasparini, F. Hepp, R.L. Moraes, F.S.F. Leite, I.A. Martins, L.B. Nascimento, D.J. Santana, I.S. Nunes, T. Silva-Soares & L.F. Toledo (2022). Avaliação do risco de extinção: Sphaenorhynchus canga Araujo-Vieira, Lacerda, Pezzuti, Leite, Assis & Cruz. https: //salve.icmbio.gov.br. Electronic version accessed 6 March 2023. DOI: https://doi.org/10.37002/salve.ficha.21923
Brasileiro, C.A., C.F.B. Haddad, R.J. Sawaya & I. Sazima (2007). A new and threatened island-dwelling species of Cycloramphus (Anura: Cycloramphidae) from southeastern Brazil. Herpetologica 63(4): 501–510. https://doi.org/10.1655/0018-0831(2007)63[501:ANATIS]2.0.CO;2 DOI: https://doi.org/10.1655/0018-0831(2007)63[501:ANATIS]2.0.CO;2
Brunes, T.O., F.C.S. Pinto, P.P.G. Taucce, M.T.T. Santos, L.B. Nascimento, D.C. Carvalho, G. Oliveira, S. Vasconcelos & F.S.F. Leite (2023). Traditional taxonomy underestimates the number of species of Bokermannohyla (Amphibia: Anura: Hylidae) diverging in the mountains of southeastern Brazil since the Miocene. Systematics and Biodiversity 21: 2156001. https://doi.org/10.1080/14772000.2022.2156001 DOI: https://doi.org/10.1080/14772000.2022.2156001
Caramaschi, U. & C.A.G. Cruz (2011). A new, possibly threatened species of Melanophryniscus Gallardo, 1961 from the state of Minas Gerais, southeastern Brazil (Amphibia, Anura, Bufonidae). Boletim do Museu Nacional 528: 1–9.
Cox, N., B.E. Young, P. Bowles, M. Fernandez, J. Marin, G. Rapacciuolo, M. Böhm, T.M. Brooks, S.B. Hedges, C. Hilton-Taylor, M. Hoffmann, R.K.B. Jenkins, M.F. Tognelli, G.J. Alexander, A. Allison, N.B. Ananjeva, M. Auliya, L.J. Avila, D.G. Chapple, D.F. Cisneros-Heredia, H.G. Cogger, G.R. Colli, A. Silva, C.C. Eisemberg, J. Els, G.A. Fong, T.D. Grant, R.A. Hitchmough, D.T. Iskandar, N. Kidera, M. Martins, S. Meiri, N.J. Mitchell, S. Molur, C.C. Nogueira, J.C. Ortiz, J. Penner, A.G.J. Rhodin, G.A. Rivas, M.O. Rödel, U. Roll, K.L. Sanders, G. Santos-Barrera, G.M. Shea, S. Spawls, B.L. Stuart, K.A. Tolley, J.F. Trape, M.A. Vidal, P. Wagner, B.P. Wallace & Y. Xie. (2022). A global reptile assessment highlights shared conservation needs of tetrapods. Nature 605: 285–290. https://doi.org/10.1038/s41586-022-04664-7 DOI: https://doi.org/10.1038/s41586-022-04664-7
Darriba, D., D. Posada, A.M. Kozlov, A. Stamatakis, B. Morel & T. Flouri (2020). ModelTest-NG: A New and Scalable Tool for the Selection of DNA and Protein Evolutionary Models. Molecular Biology and Evolution 37(1): 291–294. https://doi.org/10.1093/molbev/msz189 DOI: https://doi.org/10.1093/molbev/msz189
Delić, T., P. Trontelj, M. Rendoš & C. Fišer (2017). The importance of naming cryptic species and the conservation of endemic subterranean amphipods. Scientific Reports 7: 3391. https://doi.org/10.1038/s41598-017-02938-z DOI: https://doi.org/10.1038/s41598-017-02938-z
Edler, D., J. Klein, A. Antonelli & D. Silvestro (2021). raxmlGUI 2.0: A graphical interface and toolkit for phylogenetic analyses using RAxML. Methods in Ecology and Evolution 12: 373–377. https://doi.org/10.1111/2041-210X.13512 DOI: https://doi.org/10.1111/2041-210X.13512
Faivovich, J., C.F.B. Haddad, P.C.A. Garcia, D.R. Frost, J.A. Campbell & W.C. Wheeler (2005). Systematic review of the frog family Hylidae, with special reference to Hylinae: phylogenetic analysis and taxonomic revision. Bulletin of the American Museum of Natural History 2005(294): 1–240. DOI: https://doi.org/10.1206/0003-0090(2005)294[0001:SROTFF]2.0.CO;2
Gehara, M., C. Canedo, C.F.B. Haddad & M. Vences (2013). From widespread to microendemic: molecular and acoustic analyses show that Ischnocnema guentheri (Amphibia: Brachycephalidae) is endemic to Rio de Janeiro, Brazil. Conservation Genetics 14: 973–982. https://doi.org/10.1007/s10592-013-0488-5 DOI: https://doi.org/10.1007/s10592-013-0488-5
Guedes, T.B., J.A.R. Azevedo, C.D. Bacon, D.B. Provete & A. Antonelli (2020). Diversity, endemism, and evolutionary history of montane biotas outside the andean region, pp. 299–328. In: Rull, V. & A.C. Carnaval (eds.). Neotropical Diversification: Patterns and Processes. Fascinating Life Sciences. Springer Cham, X + 820 pp. https://doi.org/10.1007/978-3-030-31167-4 DOI: https://doi.org/10.1007/978-3-030-31167-4_13
Haddad, C.F.B., L.F. Toledo, C.P.A. Prado, D. Loebmann, J.L. Gasparini & I. Sazima (2013). Guia dos Anfíbios da Mata Atlântica: Diversidade e Biologia/Guide to the Amphibians of the Atlantic Forest: Diversity and Biology. Anolis Books, São Paulo, 544 pp.
Howard, S.D. & D.P. Bickford (2014). Amphibians over the edge: silent extinction risk of Data Deficient species. Diversity and Distributions 20: 837–846. https://doi.org/10.1111/ddi.12218 DOI: https://doi.org/10.1111/ddi.12218
ICMBio - Instituto Chico Mendes de Conservação da Biodiversidade (2018). Livro Vermelho da Fauna Brasileira Ameaçada de Extinção: Volume V – Anfíbios. Instituto Chico Mendes de Conservação da Biodiversidade, Ministério do Meio Ambiente, Brasília.
IUCN SSC Amphibian Specialist Group & Instituto Boitatá de Etnobiologia e Conservação da Fauna (2023). Sphaenorhynchus canga. The IUCN Red List of Threatened Species 2023: e.T89255741A172241454. https://doi.org/10.2305/IUCN.UK.2023-1.RLTS.T89255741A172241454.en. Electronic version accessed 15 April 2024. DOI: https://doi.org/10.2305/IUCN.UK.2023-1.RLTS.T89255741A172241454.en
Jansen, M., R. Bloch, A. Schulze & M. Pfenninger (2011). Integrative inventory of Bolivia’s lowland anurans reveals hidden diversity. Zoologica Scripta 40(6): 567–583. https://doi.org/10.1111/j.1463-6409.2011.00498.x DOI: https://doi.org/10.1111/j.1463-6409.2011.00498.x
Katoh, K. & H. Toh (2008). Recent developments in the MAFFT multiple sequence alignment program. Briefings in Bioinformatics 9: 286–298. DOI: https://doi.org/10.1093/bib/bbn013
Koroiva, R., L.R.R. Rodrigues & D.J. Santana (2020). DNA barcoding for identification of anuran species in the central region of South America. PeerJ 8: e10189. https://doi.org/10.7717/peerj.10189 DOI: https://doi.org/10.7717/peerj.10189
Koroiva, R. & D.J. Santana (2022). Evaluation of partial 12S rRNA, 16S rRNA, COI and Cytb gene sequence datasets for potential single DNA barcode for hylids (Anura: Hylidae). Anais da Academia Brasileira de Ciências 94(4): e20200825. https://doi.org/10.1590/0001-3765202220200825 DOI: https://doi.org/10.1590/0001-3765202220200825
Kumar, S., G. Stecher, M. Li, C. Knyaz & K. Tamura (2018). MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Molecular Biology and Evolution 35(6): 1547–1549. https://doi.org/10.1093/molbev/msy096 DOI: https://doi.org/10.1093/molbev/msy096
Leigh, J.W. & D. Bryant (2015). popart: full‐feature software for haplotype network construction. Methods in Ecology and Evolution 6: 1110–1116. https://doi.org/10.1111/2041-210X.12410 DOI: https://doi.org/10.1111/2041-210X.12410
Leite, F.S.F., F.A. Juncá & P.C. Eterovick (2008). Status do conhecimento, endemismo e conservação de anfíbios anuros da Cadeia do Espinhaço, Brasil. Megadiversidade 4: 158–176.
Lyra, M.L., C.F.B. Haddad & A.M.L Azeredo-Espin (2017). Meeting the challenge of DNA barcoding Neotropical amphibians: polymerase chain reaction optimization and new COI primers. Molecular Ecology Resources 17(5): 966–980. https://doi.org/10.1111/1755-0998.12648 DOI: https://doi.org/10.1111/1755-0998.12648
Magalhães, R.F., P. Lemes, A. Camargo, U. Oliveira, R.A. Brandão, H. Thomassen, P.C.A. Garcia, F.S.F. Leite & F.R. Santos (2017). Evolutionarily significant units of the critically endangered leaf frog Pithecopus ayeaye (Anura, Phyllomedusidae) are not effectively preserved by the Brazilian protected areas network. Ecology and Evolution 7: 8812–8828. https://doi.org/10.1002/ece3.3261 DOI: https://doi.org/10.1002/ece3.3261
MCTIC (2018). Resolução Normativa no 37, de 15 de fevereiro de 2018. Baixa a Diretriz da Prática de Eutanásiado Conselho Nacional de Controle de Experimentação Animal - Concea. Diário Oficial da União 36: 5.
Ministério do Meio Ambiente (2022). Portaria MMA no 148, de 7 de junho de 2022. Altera os Anexos da Portaria no 443, de 17 de dezembro de 2014, da Portaria no 444, de 17 de dezembro de 2014, e da Portaria no 445, de 17 de dezembro de 2014, referentes à atualização da Nacional de Espécies Am. Diário Oficial da União 108: 74–104.
Neves, M.O., E.A. Pereira, J.L.M.M. Sugai, S.B. Rocha, R.N. Feio & D.J. Santana (2018). Distribution pattern of anurans from three mountain complexes in southeastern Brazil and their conservation implications. Anais da Academia Brasileira de Ciências 90(2): 1611–1623. https://doi.org/10.1590/0001-3765201820170203 DOI: https://doi.org/10.1590/0001-3765201820170203
Palumbi, S., A. Martin, S. Romano, W.O. McMillan, L. Stice & G. Grabowski (2002). The simple fool’s guide to PCR. Version 2.0. University of Hawaii, Honolulu, 46 pp.
Pena, J.C.C., F. Goulart, G.W. Fernandes, D. Hoffmann, F.S.F. Leite, N.B. Santos, B. Soares-Filho, T. Sobral-Souza, M.H. Vancine & M. Rodrigues (2017). Impacts of mining activities on the potential geographic distribution of esatern Brazil mountain top endemic species. Perspectives in Ecology and Conservation 15(3): 172– 178. https://doi-org.ez25.periodicos.capes.gov.br/10.1016/j.pecon.2017.07.005 DOI: https://doi.org/10.1016/j.pecon.2017.07.005
Pereira, E.A., K. Ceron, H.R. Silva & D.J. Santana (2022). The dispersal between Amazonia and Atlantic Forest during the Early Neogene revealed by the biogeography of the treefrog tribe Sphaenorhynchini (Anura, Hylidae). Ecology and Evolution 12(4): e8754. https://doi.org/10.1002/ece3.8754 DOI: https://doi.org/10.1002/ece3.8754
Pontes, M.R. & C.E. Guidorizzi (2023). Lista Nacional de Espécies Ameaçadas de Extinção: atualizações para os anfíbios brasileiros. Herpetologia Brasileira 11: 12–20.
Puillandre, N., S. Brouillet & G. Achaz (2021). ASAP: assemble species by automatic partitioning. Molecular Ecology Resources 21(2): 609–620. https://doi.org/10.1111/1755-0998.13281 DOI: https://doi.org/10.1111/1755-0998.13281
Ribeiro, M.C., J.P. Metzger, A.C. Martensen, F.J. Ponzoni & M.M. Hirota (2009). The Brazilian Atlantic Forest: How much is left, and how is the remaining forest distributed? Implications for conservation. Biological Conservation 142(6): 1141–1153. https://doi.org/10.1016/j.biocon.2009.02.021 DOI: https://doi.org/10.1016/j.biocon.2009.02.021
Ribeiro, M.C., A.C. Martensen, J.P. Metzger, M. Tabarelli, F. Scarano & M.J. Fortin (2011). The Brazilian Atlantic Forest: A Shrinking Biodiversity Hotspot. In: Zachos, F.E. & J.C. Habel (eds.). Biodiversity Hotspots. Distribution and Protection of Conservation Priority Areas. Springer, Berlin, Heidelberg, xvii + 546 pp. DOI: https://doi.org/10.1007/978-3-642-20992-5_21
Rossa-Feres, D.C., M.V. Garey, U. Caramaschi, M.F. Napoli, F. Nomura, A.A. Bispo, C.A. Brasileiro, M.T.C. Thomé, R.J. Sawaya, C.E. Conte, C.A.G. Cruz, L.B. Nascimento, J.L. Gasparini, A.P. Almeida & C.F.B. Haddad (2017). Anfíbios da Mata Atlântica: lista de espécies, histórico dos estudos, biologia e conservação. In: Monteiro-Filho, E.L.A. & C.E. Conte (eds.). Revisões em Zoologia: Mata Atlântica. Eitora UFPR, Curitiba, 489 pp.
Sheth, B.P. & V.S. Thaker (2017). DNA barcoding and traditional taxonomy: an integrated approach for biodiversity conservation. Genome 60(7): 618–628. https://doi.org/10.1139/gen-2015-0167 DOI: https://doi.org/10.1139/gen-2015-0167
Silva, E.T.D., M.A. Peixoto, F.S.F. Leite, R.N. Feio & P.C.A. Garcia (2018). Anuran distribution in a highly diverse region of the Atlantic Forest: the Mantiqueira mountain range in southeastern Brazil. Herpetologica 74(4): 294–305. https://doi.org/10.1655/0018-0831.294 DOI: https://doi.org/10.1655/Herpetologica-D-17-00025.1
Silveira, A.L., L.S.V.B. Ribeiro, T.T. Dornas & T.N. Fernandes (2019). Novos registros geográficos, variação morfológica e notas de história natural de Scinax tripui (Amphibia, Anura, Hylidae) na Mata Atlântica de Minas Gerais (Brasil). Revista Brasileira de Zoociências 20: 1–23. https://doi.org/10.34019/2596-3325.2019.v20.27094 DOI: https://doi.org/10.34019/2596-3325.2019.v20.27094
Silveira, A.L., L.S.V.B. Ribeiro, T.T. Dornas & T.N. Fernandes (2020). Novos registros de Sphaenorhynchus canga (Amphibia, Anura, Hylidae) no Quadrilátero Ferrífero em Minas Gerais, Sudeste do Brasil. Neotropical Biology Conservation 15(1): 19–28. https://doi.org/10.3897/neotropical.15.e48718 DOI: https://doi.org/10.3897/neotropical.15.e48718
Stamatakis, A. (2014). RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30(9): 1312–1313. https://doi.org/10.1093/bioinformatics/btu033 DOI: https://doi.org/10.1093/bioinformatics/btu033
Toledo, L.F., S.P. Carvalho-e-Silva, A.M.P.T. Carvalho-e-Silva, J.L. Gasparini, D. Baêta, R. Rebouças, C.F.B. Haddad, G.G. Becker & T. Carvalho (2023). A retrospective overview of amphibian declines in Brazil’s Atlantic Forest. Biological Conservation 277: 109845. https://doi.org/10.1016/j.biocon.2022.109845 DOI: https://doi.org/10.1016/j.biocon.2022.109845
Zachos, F.E. & J.C. Habel (2011). Biodiversity Hotspots: Distribution and Protection of Conservation Priority Areas. Springer, Berlin, Heidelberg, XVII + 546 pp. DOI: https://doi.org/10.1007/978-3-642-20992-5
Zhang, J., P. Kapli, P. Pavlidis & A. Stamatakis (2013). A general species delimitation method with applications to phylogenetic placements. Bioinformatics 29(22): 2869–2876. https://doi.org/10.1093/bioinformatics/btt499 DOI: https://doi.org/10.1093/bioinformatics/btt499