DNA barcode reveals the occurrence of Palearctic Olepa schleini Witt et al., 2005 (Lepidoptera: Erebidae: Arctiinae) from peninsular India with morphological variations and a new subspecies

Main Article Content

Aparna Sureshchandra Kalawate
http://orcid.org/0000-0001-6595-6749
Shital Pawara
https://orcid.org/0000-0002-5469-5683
A. Shabnam
https://orcid.org/0000-0002-9307-3625
K.P. Dinesh
https://orcid.org/0000-0002-6700-4896

Abstract

The present study was taken up to report a new record of the tiger moth genus, Olepa Watson, 1980 from India along with the discovery of a new subspecies.  Earlier  the genus was thought to have restricted distribution range in South and South-East Asia until the report of O. schleini Witt, Müller, Kravchenko, Miller, Hausmann & Speidel from the Mediterranean Coastal Plain of Israel in 2005. The species identification and the new subspecies is proposed based on the combination of morphological studies, available literature comparisons, geographical distribution,  DNA barcoding and its phylogeny. Morphological character crypticity and genital structure variations are well documented in the genus with ‘bio-species’ groups. DNA Barcoding data of mt COI has provided some resolution in sorting the problems of ‘bio-species’ groups of the genus in the past studies. In the present study, with the available  mt DNA COI barcodes and newly generated barcodes genetic identity is confirmed for the species O. ricini, O. schleini, O. toulgoeti and Olepa schleini chandrai ssp. nov., with their phylogenetic relationships. Morphological variations within the O. schleini species complex are discussed with a new record of the species for India and a new subspecies description. With the first mt COI barcode phylogeny for the genus, comments are made on the taxonomic identity of the mt COI DNA barcodes available in the GenBank for the Olepa species from India.


 

Article Details

How to Cite
[1]
Kalawate, A.S., Shital Pawara, A. Shabnam and K.P. Dinesh 2020. DNA barcode reveals the occurrence of Palearctic Olepa schleini Witt et al., 2005 (Lepidoptera: Erebidae: Arctiinae) from peninsular India with morphological variations and a new subspecies. Journal of Threatened Taxa. 12, 9 (Jun. 2020), 16143–16152. DOI:https://doi.org/10.11609/jott.5596.12.9.16143-16152.
Section
Short Communications
Author Biographies

Aparna Sureshchandra Kalawate, Zoological Survey of India, Western Regional Centre, Vidya Nagar, Sector-29, P.C.N.T. (PO), Rawet Road, Akurdi, Pune, Maharashtra 411044, India.

Entomology

Shital Pawara, S.G. Patil Arts, Science & Commerce College, Sakri, Maharashtra 424304, India.

PhD. Student

A. Shabnam, Zoological Survey of India, Western Regional Centre, Vidya Nagar, Sector-29, P.C.N.T. (PO), Rawet Road, Akurdi, Pune, Maharashtra 411044, India.

Research scholar

K.P. Dinesh, Zoological Survey of India, Western Regional Centre, Vidya Nagar, Sector-29, P.C.N.T. (PO), Rawet Road, Akurdi, Pune, Maharashtra 411044, India.

Scientist-D

References

Ashfaq, M., S. Akhtar, M.A. Rafi, S. Mansoor & P.D. Hebert (2017). Mapping global biodiversity connections with DNA barcodes: Lepidoptera of Pakistan. PLoS ONE 12(3): e0174749. https://doi.org/10.1371/journal.pone.0174749 DOI: https://doi.org/10.1371/journal.pone.0174749

Dubatolov, V.V. (2011). A new Olepa Watson, 1980 species from South India (Lepidoptera, Arctiidae). Atalanta 42 (1/2) : 136–137.

Fabricius, J.C. (1775). Systema Entomologiae, sistens Insectorum Classes, Ordines, Genera, Species, Adiectis Synonymis, Locis, Descriptionibus, Observationibus. Kortii, Flensbvrgi et Lipsidae [= Flensburg and Leipzig]: [32]+832pp.

Hadley, A. (2010). Combine ZP software, new version. Available from: http://www.hadleyweb.pwp.blueyonder.co.uk/CZP/News.htm (accessed 16 August 2018).

Haines, W.P. & D. Rubinoff (2012). Molecular phylogenetics of the moth genus Omiodes Guenée (Crambidae: Spilomelinae), and the origins of the Hawaiian lineage. Molecular Phylogenetics and Evolution 65(1): 305–316.

Hebert, P.D., A. Cywinska, S.L. Ball & J.R. Dewaard (2003). Biological identifications through DNA barcodes. Proceedings of the Royal Society of London. Series B: Biological Sciences 270(1512): 313–321. https://doi.org/10.1098/rspb.2002.2218 DOI: https://doi.org/10.1098/rspb.2002.2218

Hebert, P.D., E.H. Penton, J.M. Burns, D.H. Janzen & W. Hallwachs (2004). Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator. Proceedings of the National Academy of Sciences 101(41): 14812–14817. https://doi.org/10.1073/pnas.0406166101. DOI: https://doi.org/10.1073/pnas.0406166101

Hebert, P.D., J.R. Dewaard, E.V. Zakharov, S.W. Prosser, J.E. Sones, J.T. McKeown, B. Mantle & J. La Salle (2013). A DNA ‘barcode blitz’: rapid digitization and sequencing of a natural history collection. PLoS ONE 8(7): e68535. https://doi.org/10.1371/journal.pone.0068535 DOI: https://doi.org/10.1371/journal.pone.0068535

ICAR-NBAIR (2019). Olepa ricini (Fabricius) National Bureau of Agricultural Insect Resources. Available from: http://www.nbair.res.in/insectpests/credits.php (accessed on 18 September 2019).

Klots, A.B. (1970). Lepidoptera, pp. 115–130. In: Tuxen, S.L. (ed.). Taxonomist’s glossary of genitalia in insects. Munksgaard, Copenhagen, 359pp.

Orhant, G. (1986). Deuxième contribution à l’étude des lépidoptèreshétérocères du sud-estAsiatique (1) Le complexed’espèces “ricini Fabricius” (Arctiidae: Arctiinae). Bulletin Society Science National France 50: 9–22.

Orhant, G. (2000). Unnouvel Olepa de l’Inde (Lepidoptera, Arctiidae, Arctiinae). Lambillionea100 (2): 269–270.

Orhant, G. (2012). Olepa neumuthi new species from Thailand (Lepidoptera: Arctiidae). Bulletin Society Entomology Mulhouse 68(4): 61–64.

Pekarsky, O. (2012). Two new species of Pseudohadena Alphéraky, 1889 from Kazakhstan (Lepidoptera, Noctuidae, Xyleninae). ZooKeys 187: 9–34. https://doi.org/10.3897/zookeys.187.2661 DOI: https://doi.org/10.3897/zookeys.187.2661

Posada, D. (2008). J Model Test: Phylogenetic Model Averaging. Molecular Biology and Evolution 25: 1253−1256. https://doi.org/10.1093/molbev/msn083 DOI: https://doi.org/10.1093/molbev/msn083

Robinson, G.S. (1976). The preparation of slides of Lepidoptera genitalia with special reference to the Microlepidoptera. Entomologist’s Gazette 27: 127–132.

Rönkä, K., J. Mappes, L. Kaila & N. Wahlberg (2016). Putting Parasemia in its phylogenetic place: a molecular analysis of the subtribe Arctiina (Lepidoptera). Systematic Entomology 41(4): 844–853.

Sambrook, J. & D. Russell (2001). Molecular Cloning: A Laboratory Manual, 3rd Edition. 619 Cold Spring Harbor, 2100pp.

Singh, N. & J. Singh (2013). Review of the genus Olepa Watson (Lepidoptera: Erebidae: Arctiinae). Tinea 22(4): 272–277.

Silvestro, D. & I. Michalak (2012). Raxml GUI: A graphical front-end for RAxML. Organisms Diversity & Evolution 12: 335−337. https://doi.org/10.1007/s13127-011-0056-0 DOI: https://doi.org/10.1007/s13127-011-0056-0

Swinhoe, C. (1885). Proceedings of the general meetings for scientific business of the Zoological Society of London, 295pp.

Tamura, K., D. Peterson, N. Peterson, G. Stecher, M. Nei & S. Kumar (2011). MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance and Maximum Parsimony Methods. Molecular Biology and Evolution 28: 2731−2739. https://doi.org/10.1093/molbev/msr121 DOI: https://doi.org/10.1093/molbev/msr121

Walker, F. (1855). List of the specimens of Lepidopterous insects in the collection of the British Museum 3: 583−775.

Witt, T.J., G.C. Müller, V.D. Kravchenko, M.A. Miller, A. Hausmann & W. Speidel (2005). A new Olepa species from Israel (Lepidoptera, Arctiidae). Nachrichtenblatt der Bayerischen. Entomologen 54(3/4): 101–115. http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.463.9720&rep=rep1&type=pdf