Prevalence of Listeria species including L. monocytogenes from apparently healthy animals at Baroda Zoo, Gujarat State, India

Authors

  • M.M. Yadav
  • A. Roy
  • B. Bhanderi
  • R.G. Jani

DOI:

https://doi.org/10.11609/JoTT.o2094.1929-35

Abstract

Listeriosis is a infectious bacterial disease of domestic and wild animals and humans. A total of 56 faecal samples were collected from mammals and birds at Baroda Zoo, Vadodara, Gujarat State, India. Confirmation of the isolates was based on biochemical tests followed by phenotypic characterization by hemolysis on sheep blood agar, Christie Atkins Munch-Petersen (CAMP) test, phosphatidylinositol-specific phospholipase C (PI-PLC) assay and phosphatidylcholine-specific phospholipase C (PC-PLC) assay. The isolates were subjected to genotypic characterization with the help of polymerase chain reaction (PCR) assay for five virulence-associated genes, plcA, prfA, hlyA, actA and iap. Listeria monocytogenes isolates were further subjected to multiplex-PCR based serotyping. From 56 samples three (5.36%) were found positive for Listeria spp. of which one (1.79%) was identified as L. monocytogenes and two (3.57%) were identified as L. innocua. The isolate of L. monocytogenes was hemolytic, CAMP positive, PI-PLC positive, hlyA, pclA and prfA positive by PCR and turned out to be PC-PLC positive and was serotyped as 4b.

Author Biographies

M.M. Yadav

Mahendra Mohan Yadav is currently working as a Assistant Professor at Research Cum Development Project on Cattle, Mahatma Phule Krishi Vidyapeeth, Rahuri, Maharashtra State and engaged in Diagnostic and Treatment aspect of Ruminants

A. Roy

Ashish Roy is currently working as a Professor at Department of Veterinary Microbiology, College of Veterinary Science, Anand, and involved in teaching and research activities of Veterinary Microbiology at Anand, Gujarat State.

B. Bhanderi

Bharat Bhanderi is currently working as a Veterinary Officer in Gujarat State. He completed his Ph.D in Veterinary Microbiology at Anand, Gujarat State.

R.G. Jani

R.G. Jani is currently working as a Professor in Department of Veterinary Medicine at College of Veterinary Science, Anand, and involved in teaching and research activities of Veterinary Medicine at Anand, Gujarat State. He is also Wildlife co-ordinator of West Zone of India.

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Published

26-07-2011

Issue

Vol. 3 No. 7 (2011)

Section

Communications

License

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