Morphometry and feeding notes of an endemic frog Amolops spinapectoralis (Amphibia: Ranidae) from Hue City, central Vietnam
DOI:
https://doi.org/10.11609/jott.10064.17.12.28011-28025Keywords:
Cylindrical spines, diet composition, feeding ecology, morphological feature, ranid frog, prey diversity, prey moss, sexual variationAbstract
This study presents the first record of the endemic ranid frog Amolops spinapectoralis in Hue City, Vietnam. Notably, the specimens collected exhibit cylindrical spines, a distinct morphological feature differing from the conical spines originally described, highlighting the species’ local morphological diversity. Given the limited ecological data on this species, we analyzed its morphological characteristics and investigated the correlation between body size and feeding ecology, as well as sexual variation in diet composition and prey diversity. We identified 507 prey items across 19 prey categories. The dominant prey taxa included Orthoptera, Coleoptera, Diptera, Formicidae, Isoptera, insect larvae, and Hymenoptera, which collectively accounted for 71.16% of the frequency of occurrence, 68.24% of the total prey number, and 66.89% of the total volume, with a relative importance index of 68.77%. Furthermore, prey mass was positively correlated with temperature and relative humidity, independent of rainfall.
References
Beaupre, S.J., E.R. Jacobson, H.B. Lillywhite & K. Zamudio (2004). Guidelines for use of live amphibians and reptiles in field and laboratory research. The Herpetological Animal Care and Use Committee (HACC) of the American Society of Ichthyologists and Herpetologists, 43 pp.
Benson, D.A., M. Cavanaugh, K. Clark, I. Karsch-Mizrachi, D.J. Lipman, J. Ostell & E.W. Sayers (2017). GenBank. Nucleic Acids Research 45(D1): D37–D42. https://doi.org/10.1093/nar/gkw1070
Berenbaum, M.R. (1996). Bugs in the System: Insects and Their Impact on Human Affairs. Basic Books, New York, USA, 377 pp.
Biavati, G.M., H.C. Wiederhecker & G.R. Colli (2004). Diet of Epipedobates flavopictus (Anura: Dendrobatidae) in a neotropical savanna. Journal of Herpetology 38(4): 510–518. https://doi.org/10.1670/30-04A
Browne, K.R. (2009). Amphibian Diet and Nutrition. Royal Zoological Society of Antwerp, Belgium, 5 pp.
Brusca, R.C., W. Moore & S.M. Shuster (2016). Invertebrates. 3rd Edition. Oxford University Press, UK, 1104 pp.
Caldart, V.M., S. Iop, T.R.N. Bertaso & C. Zanini (2012). Feeding ecology of Crossodactylus schmidti (Anura: Hylodidae) in southern Brazil. Zoological Studies 51(4): 484–493.
Duellman, W.E. & L. Trueb (1994). Biology of Amphibians. McGraw-Hill, Baltimore–London, 670 pp.
Duong, D.L. & D.C. Ngo (2022). New records and an updated checklist of amphibians from Binh Dinh Province, Central-southern Vietnam. Herpetology Notes 15: 751–762.
Forsman, A. (1996). Body size and net energy gain in gape-limited predators: a model. Journal of Herpetology 30(3): 307–319. https://doi.org/10.2307/1565167
Frost, D.R. (2025). Amphibian Species of the World: an Online Reference. Version 6.2. American Museum of Natural History, New York, USA. Electronic Database accessible at https://amphibiansoftheworld.amnh.org/Amphibia/Anura/Ranidae/Amolops/Amolops-spinapectoralis (accessed on 21.v.2025).
Hadfield, C.A., L.A. Clayton & S.L. Barnett (2006). Nutritional support of amphibians. Journal of Exotic Pet Medicine 15(4): 255–263. https://doi.org/10.1053/j.jepm.2006.09.004
Inger, R.F., N.L. Orlov & I.S. Darevsky (1999). Frogs of Vietnam: A report on new collections. Fieldiana Zoology (New Series) 92: 1–46.
Johnson, N.F. & C.A. Triplehorn (2005). Borror and Delong’s Introduction to the Study of Insects. 7th Edition. Thomson Learning, Inc., California, USA, 864 pp.
Krebs, C.J. (1999). Ecological Methodology. Addison Wesley Longman, Menlo Park, California, USA, 690 pp.
Le, T.T.D., J.J.L. Rowley, T.A.D. Tran & D.H. Hoang (2019). The diet of a forest-dependent frog species, Odorrana morafkai (Anura: Ranidae), in relation to habitat disturbance. Amphibia-Reptilia 41(1): 29–41. https://doi.org/10.1163/15685381-20191171
Leavitt, D.J. & L.A. Fitzgerald (2009). Diet of nonnative Hyla cinerea in a Chihuahuan desert wetland. Journal of Herpetology 43(3): 541–545. https://doi.org/10.1670/08-225R1.1
Lyu, Z.T., J. Wu, J. Wang, Y.H. Sung, Z.Y. Liu, Z.C. Zeng, X. Wang, Y.Y. Li & Y.Y. Wang (2018). New species of Amolops (Anura: Ranidae) from southwestern Guangdong, China. Zootaxa 4418(6): 562–576. https://doi.org/10.11646/zootaxa.4418.6.4
Magnusson, W.E., A.P. Lima, W.A. Silva & M.C. Araújo (2003). Use of geometric forms to estimate volume of invertebrates in ecological studies of dietary overlap. Copeia 2003(1): 13–19. https://doi.org/10.1643/0045-8511(2003)003%5B0013:uogfte%5D2.0.co;2
Magurran, A.E. (2004). Measuring Biological Diversity. Blackwell Science, Malden, Massachusetts, USA, 256 pp.
McWilliams, D.A. (2008). Nutrition Recommendations for some Captive Amphibian Species (Anura and Caudata). The Canadian Association of Zoos and Aquariums Nutrition Advisory and Research Group (GARN-NARG).
Millar, I.M., V.M. Uys & R.P. Urban (2000). Collecting and Preserving Insects and Arachnids. SDC, Switzerland.
Ngo, V.B. & D.C. Ngo (2011). Morphological characters, sexual ratio, testis, and egg development of Quasipaa verrucospinosa (Bourret, 1937) (Amphibia: Anura: Dicroglossidae) from Thua Thien-Hue province, central Vietnam. Russian Journal of Herpetology 18(2): 157–164. https://doi.org/10.30906/1026-2296-2011-18-2-157-164
Ngo, V.B., D.C. Ngo, T.X. Nguyen, L.P.-C. Hou & T.N. Tran (2012). Advertisement calls and reproductive activity of Hylarana guentheri (Boulenger, 1882) from Bach Ma National Park. Russian Journal of Herpetology 19(3): 239–250. https://doi.org/10.30906/1026-2296-2012-19-3-239–250
Ngo, V.B., D.C. Ngo & L.P.-C. Hou (2013). Reproductive ecology of Quasipaa verrucospinosa (Bourret, 1937): Living in the tropical rain forests of Central Vietnam. Journal of Herpetology 47(1): 138–147. https://doi.org/10.1670/11-286
Ngo, V.B., Y.-F. Lee & D.C. Ngo (2014). Variation in dietary composition of granular spiny frogs (Quasipaa verrucospinosa) in central Vietnam. The Herpetological Journal 24(3): 245–253.
Nguyen, V., D.H. Truong, T.L. Hoang, V.H. Nguyen, D.V. Phung, H.K. Ha, D.H. Tran, V.H. Phan, D.A. Nguyen & Q.V. Le (2004). The Climatic-Hydrology Characters of Thua Thien-Hue Province. Thuan Hoa Publishing House, Vietnam.
Nguyen, S.V., C.T. Ho & T.Q. Nguyen (2009). Herpetofauna of Vietnam. Chimaira, Frankfurt, 768 pp.
Pham, C.T., A. Dogra, A. Gawor, A. Rauhaus, G. Kloeble, T.Q. Nguyen & T. Ziegler (2015). First record of Amolops cremnobatus from Thanh Hoa Province, Vietnam, including an extended tadpole description and the first larval staging for Amolops. Salamandra 51(2): 111–120.
Pham, V.A., D.C. Ngo, Q.T. Nguyen & V.B. Ngo (2022). Diet of Microhyla butleri and M. heymonsi from Son La Province, northwestern Vietnam. Herpetological Conservation and Biology 17(1): 217–224.
Pham, V.A., V.B. Ngo, V.T. Tran, D.L. Doan, D.C. Ngo & Q.T. Nguyen (2023a). Feeding ecology of the big headed treefrog, Polypedates megacephalus (Amphibia: Anura: Rhacophoridae), from Northwestern Vietnam. Russian Journal of Herpetology 30(3): 175–183. https://doi.org/10.30906/1026-2296-2023-30-3-175-183
Pham, V.A., Q.T. Nguyen, B.N. Sung, V.T. Tran, D.M. Le & N.H. Ngo (2023b). New record and dietary ecology of an endangered amphibian species, Micryletta nigromaculata Poyarkov, Nguyen, Duong, Gorin & Yang, 2018, from Vietnam. Acta Herpetologica 18(2): 131–138. https://doi.org/10.36253/a_h-14516
Pincheira-Donoso, D., L.P. Harvey, F. Grattarola & M. Jara (2020). The multiple origins of sexual size dimorphism in global amphibians. Global Ecology and Biogeography 30(2): 1–29. https://doi.org/10.1111/geb.13230
Rao, D.Q. & J.A. Wilkinson (2007). A new species of Amolops (Anura: Ranidae) from southwest China. Copeia 2007(4): 913–919. https://doi.org/10.1643/0045-8511(2007)7[913:ANSOAA]2.0.CO;2
Redford, K.H. & J.G. Dorea (1984). The nutritional value of invertebrates with emphasis on ants and termites as food for mammals. Journal of Zoology 203: 385–395. https://doi.org/10.1111/j.1469-7998.1984.tb02339.x
Rosner, B. (2010). Fundamentals of Biostatistics. Cengage Learning, Inc., Kentucky, USA, 888 pp.
Rumpold, B.D. & O.K. Schlüter (2013). Potential and challenges of insects as an innovative source for food and feed production. Innovative Food Science and Emerging Technologies 17: 1–11. https://doi.org/10.1016/j.ifset.2012.11.005
Sengupta, S., B. Hussain, P.K. Choudhury, J. Gogoi, M.F. Ahmed & N.K. Choudhury (2008). A new species of Amolops (Anura: Ranidae) from Assam, North-Eastern India. Hamadryad 32(1): 5–12.
Solé, M., O. Beckmann, B. Pelz, A. Kwet & W. Egels (2005). Stomach-flushing for diet analysis in anuran: an improved protocol evaluated in a case study in Araucaria forests, southern Brazil. Studies on Neotropical Fauna and Environment 40(1): 23–28.
Thai, T.B. (2003). Invertebrates. Education Publishing House, Hanoi, Vietnam.
Valderrama-Vernaza, M., M.P. Ramírez-Pinilla & V.H. Serrano-Cardozo (2009). Diet of the Andean frog Ranitomeya virolinensis (Athesphatanura: Dendrobatidae). Journal of Herpetology 43(1): 114–123. https://doi.org/10.1670/07-247R1.1
Wells, K.D. (2007). The Ecology and Behavior of Amphibians. University of Chicago Press, Chicago, 1148 pp. https://doi.org/10.7208/chicago/9780226893334.001.0001
Yousaf, S., T. Mahmood, M. Rais & I.Z. Qureshi (2010). Population variation and food habits of ranid frogs in the rice-based cropping system in Gujranwala Region, Pakistan. Asian Herpetological Research 1(2): 122–130.
Zar, J.H. (2010). Biostatistical Analysis. 5th Edition. Prentice Hall, Upper Saddle River, New Jersey, USA, 944 pp.
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