Pollination biology of Impatiens cuspidate Wight and Arn. (Balsaminaceae), a rare and endemic balsam of the Western Ghats, India

Abstract

The pollination biology of Impatiens cuspidata, a rare and endemic balsam from the Western Ghats, has been studied with special reference to phenology, pollination, pollen-pistil interactions, breeding experiments and stigma receptivity. It flowers at night between 2330 and 0430 hr; flowering days extend up to 160 days in a year. The anther dehisced one day before anthesis, which confirmed the protandrous condition of the flower. Pollen-ovule ratio was calculated as 1729:1. Hawk moths, honeybees, flies and butterflies are the major pollinators of Impatiens cuspidata. Pollen grains are oval, having an average diameter of 28.24μm. Pollen viability by FCR test confirmed that 82% pollen grains are viable on the day of anthesis. Best pollen germination along with 1636μm tube development was achieved in Brewbakers medium. Stigma was more receptive (up to 80%) on the first day of flower opening. It chiefly reproduced by means of cross pollination, where the fruit set was only 40%, but artificial cross-pollination through xenogamy enhanced fruit set up to 80%. The plant is an obligate out-crosser and self incompatible, as confirmed by various hand pollination experiments. Seed germination in natural and controlled conditions was only 20%. Its dependence on a specialized habitat, bottlenecks in sexual reproduction, low percentage of seed germination and other abiotic factors could be reasons for its limited distribution and endemism.