Journal of Threatened Taxa |
www.threatenedtaxa.org | 26 September 2020 | 12(13): 16924–16926
ISSN 0974-7907 (Online) | ISSN
0974-7893 (Print)
doi: https://doi.org/10.11609/jott.5447.12.13.16924-16926
#5447 | Received 03 October 2019
| Final received 25 April 2020 | Finally accepted 28 August 2020
First report of mango leaf gall midge Procontarinia robusta
Li, Bu & Zhang (Diptera: Cecidomyiidae)
from India
Duraikannu Vasanthakumar
1, Senthilkumar Palanisamy
2 & Radheshyam Murlidhar
Sharma 3
1,3 Zoological Survey of India,
Western Regional Centre, Pune, Maharashtra 411044, India.
2 Department of Genetic
Engineering, SRM Institute of Science and Technology, Kattankulathur,
Tamilnadu 603203, India.
1 duraivasanthakumar@gmail.com
(corresponding author), 2 mpsenthilkumar@gmail.com, 3 rmsharma13@gmail.com
Editor: R. Ramanibai,
University of Madras, Chennai, India. Date of publication: 26 September
2020 (online & print)
Citation: Vasanthakumar,
D., S. Palanisamy & R.M. Sharma (2020). First report of mango leaf gall
midge Procontarinia robusta
Li, Bu & Zhang (Diptera: Cecidomyiidae)
from India. Journal of Threatened Taxa 12(13): 16924–16926. https://doi.org/10.11609/jott.5447.12.13.16924-16926
Copyright: © Vasanthakumar et al. 2020. Creative Commons Attribution
4.0 International License. JoTT allows unrestricted use, reproduction, and distribution
of this article in any medium by providing adequate credit to the author(s) and
the source of publication.
Funding: None.
Competing interests: The authors declare no
competing interests.
Acknowledgements: We express our
sincere thanks to Dr. Peter Kolesik
for his immediate response when contacted to clarify the identity of the
gall. We are grateful to the director,
Zoological Survey of India, Kolkata and officer-in-charge, Western Regional
Centre, ZSI, Pune for providing the necessary facilities. Thanks are also due to the staff of WRC, ZSI,
Pune, for their constant encouragement.
Currently, 22 species of gall midges (Diptera:
Cecidomyiidae) are known to be associated with mango Mangifera indica L.
(Anacardiaceae) (Gagné
& Jaschhof 2017; Jiao et al. 2018) which includes
16 species belonging to the genus Procontarinia. Procontarinia
robusta Li, Bu & Zhang known for its gall on
the leaves of mango tree, was described from China in the year 2003 based on
adult male and female (Li et al. 2003).
Later, immature stages of this species were described by Cai et al.
(2013). Even though a similar type of
gall on mango is known from India (Jharkhand and Andhra Pradesh) (Kieffer 1909;
Mani 1959), the identity of the causative agent is not known. Cai et al. (2013) have considered the
causative agent of this gall as P. robusta
based on the illustrations given by Kieffer (1909) and Mani (1959). The checklist of Indian gall midges revealed
394 species which includes 11 species of Procontarinia
but the P. robusta has not been included in
the list as it is known only from gall morphology not from insect life stages
(Sharma 2009). Here, we report the
occurrence of P. robusta in India based on the
larva and pupa for the first time.
Mango leaves with cylindrical galls mentioned by Kieffer 1909 & Mani
1959 were collected from different localities (in the vicinity of Tamhini, Pune District, Maharashtra; Guntur, Andhra
Pradesh; and Singanallur Lake area, Coimbatore
District, Tamil Nadu) (Figure 1) and transferred to the laboratory. Some galls were cut open to obtain immature
stages (larvae and pupae) and the remaining galls were kept in a plastic bag to
rear into adults. As adults had not
emerged from the galls, efforts were made to identify the larva and pupa. The larvae and pupae were cleared by using
10% KOH and processed to prepare slides as per the method described by Kolesik et al. (2009).
The slides mounted in Canada balsam were deposited in the National
Zoological Collections of ZSI, Western Regional Centre, Pune.
Material examined: Larva and pupa (2 numbers each dissected and mounted
on slides) collected as leaf galls (Ent 10/210) from Guntur, Andhra Pradesh
(16.3030N & 80.4820E) on 29.v.2018, coll. Senthilkumar; two larvae in alcohol collected as gal (Ent
10/211) on 22.ix.2018 from Singanallur Lake area,
Coimbatore, Tamil Nadu (10.9940N & 77.0240E), coll. Vasanthakumar D.; larvae and pupae (three numbers each in
alcohol) collected as gall (Ent 10/212) on 09.viii.2019 from vicinity of Mulshi, Pune District, Maharashtra) (18.5010N
& 73.5130E), coll. D. Vasanthakumar.
Diagnosis: Colour of larvae yellowish-white (Image 1A), antennae short,
as long as wide. Sternal spatula long,
narrow (270 μm) and trilobed, with large central lobe
and minute lateral lobes (Image 1 B). No
visible papillae on terminal segment as well as near sternal spatula as
described by Cai et al. (2013). Antennal
horns of pupa prominent, outer edge serrated (Image 1C). Prothoracic spiracle short, as long as
wide. No visible facial papillae.
Gall: Leaf gall (Image 1D).
Epiphyllous, cylindrical often obtusely conical, sessile, glabrous, brown to reddish-brown, shiny, hard, unilocular
(Image 1E), indehiscent galls, 1mm in diameter at the base and 1–2.5 mm in
height. The leaf epidermis bursts when the gall develops, leaving a structure
resembling a calyx around the base. On
the underside of the gall is a slightly discoloured blister with a necrotic
area in the centre (Image 1F). At gall
maturity, a round necrotic area appears at the gall’s apex. Pupation takes
place inside the gall. The pupal skin
can be seen attached to the emergence hole (Image 1G) (Mani 2000).
Distribution: China, Indonesia, East Timor (Cai et al. 2013). India (new
record)
Notes: P. robusta can be easily
identified from its larva. A large
central anterior lobe accompanied by a small lobe on either side of sternal spatula
of larva is the key character to identify this species as described by Cai et
al. (2013).
For
image & figure – click here
References
Cai, H.J., P. Kolesik,
H.Y. Wang, F.R. Liao, V. Quintao, K.M. Harris &
G.A. Bellis (2013). Description of the
immature stages and gall morphology, and molecular characterisation of Procontarinia robusta,
a gall midge (Diptera: Cecidomyiidae)
damaging leaves of mango Mangifera indica L. (Anarcadiaceae) in
southern Asia. Australian Journal of Entomology 52: 206–211.
Gagné, R.J. & M. Jaschhof (2017). A Catalog of the Cecidomyiidae
(Diptera) of the World, 4th Edition.
Digital, USDA, Washington, 762pp.
Jiao, K.L., H. Wang,
D.W. Wei, J.Y. Mo, Y.H. Wang, W.J. Bu & P. Kolesik (2018). A new species of Procontarinia (Diptera: Cecidomyiidae) damaging
fruit of mango, Mangifera indica (Anacardiaceae), in
China. Zootaxa 4413(2): 368–376.
Kieffer, J.J. (1909). Description de galles et d’insectes
gallicoles d’Asie. Marcellia 7: 149–167.
Kolesik, P., A.D. Rice, G.A. Bellis & M.G. Wirthensohn
(2009). Procontarinia
pustulata, a new species of gall midge (Diptera: Cecidomyiidae) feeding
on mango Mangifera indica
(Anarcadiaceae), in northern Australia and Papua New
Guinea. Australian Journal of Entomology 48: 310–316.
Li, J., W.J. Bu & Q.Y. Zhang
(2003). A new species of gall midge (Diptera: Cecidomyiidae) attacking
mango leaves from China. Acta Zootaxonomica Sinica 28: 148–151.
Mani, M.S. (1959). Cecidotheca Indica. Agra University
Journal of Research (Science) 8: 91–279.
Sharma, R.M. (2009).
Checklist of Indian Gall midges (Diptera: Cecidomyiidae).
http://www.zsi.gov.in/zoologicalsurveyofindia/zsidata/checklist. Digital,
Zoological Survey of India, Kolkata, 23pp.