A note on five freshwater sponges (Porifera:Spongillina: Spongillidae)
from Pune, Maharashtra, India
Shriraj S. Jakhalekar1 & H. V. Ghate 2
1,2 Post-Graduate Research
Centre, Department of Zoology, Modern College of Arts, Science & Commerce, Shivajinagar, Pune, Maharashtra 411005, India
1 shriraj.jak@gmail.com, 2 hemantghate@gmail.com
(corresponding author)
doi: http://dx.doi.org/10.11609/JoTT.o3356.4392-403| ZooBank: urn:lsid:zoobank.org:pub:1C5DEFC7-41F8-49FC-B6A5-6DDD2760E4A4
Editor: Alexander Ereskovsky,
IMBE, Marseille, France. Date of publication: 26 May 2013 (online
& print)
Manuscript details: Ms #
o3356 | Received 21 September 2012 | Final received 20 December 2012 | Finally
accepted 21 April 2013
Citation: Jakhalekar,
S.S. & H.V. Ghate (2013). A
note on five freshwater sponges (Porifera: Spongillina: Spongillidae) from
Pune, Maharashtra, India. Journal of Threatened Taxa 5(9): 4392-4403; http://dx.doi.org/10.11609/JoTT.o3356.4392-403
Copyright: © Jakhalekar& Ghate 2013. Creative Commons
Attribution 3.0 Unported License. JoTT allows unrestricted use of this article in any medium,
reproduction and distribution by providing adequate credit to the authors and
the source of publication.
Funding: Self funded.
Competing Interest: None.
Acknowledgements: The
authors express their deepest gratitude to Dr. Klaus Ruetzler(USA) and Dr. Alexander Ereskovsky (France) for
providing valuable literature and reviewing the first draft of the manuscript.
Dr. R. Sharma (Officer-In-Charge, ZSI, Pune) permitted access to the library at
the Zoological Survey of India, Pune; Mr. Jadhav and
Ms. S. Paripatyadar extended their help during the
referencing work. Sincere thanks to them. SSJ is
particularly thankful to his lab-mates, Girish,
Sameer, Yugandhar, Swapniland Mihir, for their help in the field and during
sample preparation for SEM and photography. We thank the Department of Physics,
University of Pune, for use of the SEM facility and Mr. Shindefor technical assistance. The authors are also grateful to the authorities of
the Modern College for facilities and encouragement.
Abstract: We report the presence of five freshwater sponges in ephemeral
and permanent fresh water bodies in and around Pune, Maharashtra, India. Field observations, descriptive notes,
habitus photographs and SEM photographs of body spicules, gemmulespicules and gemmules of five species are provided. Eunapius carteri (Bowerbank,
1863), Radiospongilla cerebellata(Bowerbank, 1863) and Corvospongilla lapidosa (Annandale, 1908) are commonly occurring
species, whereas Ephydatia meyeni (Carter, 1849) and Dosilia plumosa (Carter, 1849) are rarely found. This report fills a longstanding gap in
observations of freshwater sponges near Pune, and it is heartening to note that
species reported earlier are still found in these environs.
Keywords: Corvospongillalapidosa, descriptive notes, Dosilia plumosa, Eunapius carteri, Ephydatia meyeni, Pune, Radiospongilla cerebellata, SEM, Spongillidae.
Phylum Porifera,
members of which are commonly known as sponges, consists of over 8500 species
distributed worldwide, mostly in marine environments. Sponges are aquatic,
sessile filter-feeding invertebrates that may also utilize dissolved organic
matter from water, and some may be carnivorous. Over 83% of species belong to Class Demospongiae, of which the vast majority
are marine although there are many species of freshwater sponges (see
van Soest et al. 2012, for a detailed overview). All freshwater sponges are now grouped
under suborder Spongillina (Manconi& Pronzato 2002) of the order Haplosclerida,
and this suborder includes 44 genera classified under six families of extant
sponges, of which the Oriental Region possesses 11 genera and 37 species
belonging to two families, namely Spongillidae and Metaniidae (Manconi & Pronzato 2008). The suborder Spongillina has been defined by Manconi & Pronzato (2002) as
containing exclusively freshwater sponges that possess megascleres—oxeas or strongyles—that
are smooth or spined, forming pauci-
to multi-spicular tracts producing, irregular to
regular meshes, occasionally with large alveolatecavities; where spongin material is mostly sparse; microscleres present or absent, including smooth or spined oxeas, aster-like or birotule like spicules. Only four families produce gemmules or resting bodies that contain totipotent cells
enclosed in protective cover. Thesegemmules, which allow the sponges to survive
desiccation, often contain special spicules or gemmuloscleresof diverse morphology that are used in identification of species (see Manconi & Pronzato 2002, for
a detailed review and keys to the families / genera of freshwater
sponges). Both
body and gemmule spicules as well as gemmule morphology are thus important structures and need
attention. Considerable work on
sponges is being done around the world and there is an online World Porifera Database at: www.marinespecies.org/porifera/ (van Soest et al. 2013). In a recent paper on global diversity of freshwater sponges Manconi & Pronzato (2008)
mention that the total number of species is 219, and of these 103 (i.e., 47%)
are endemic.
All over the world there is
an increasing interest in study of sponges, or ‘Spongology,’
which has been defined as the study of all aspects of their biology, ecology
and taxonomy. As mentioned by van Soest et al. (2012), apart from academic interest sponges
can produce chemical compounds with pharmaceutical properties, including some
with anti-tumor, anti-infective and anti-inflammatory properties. There is thus a need to study our sponge
fauna.
After the pioneering work on
diversity and biology of the sponges of the Indian subcontinent by Annandale
(1911), no significant addition or contribution has been made by later workers; especially with respect to the knowledge of systematics
and biology of Indian freshwater sponges. Annandale listed 23 species of freshwater sponges under seven genera
(including subspecies) in the main text and added two more as an addenda, and
gave detailed notes about their distribution and biology in his classic
‘Freshwater Sponges, Hydroids & Polyzoa’ in ‘The
Fauna of British India’ volume. Penney & Racek (1968), in their
‘Comprehensive Revision of a Worldwide Collection of Freshwater Sponges’, added
substantial information; they also revised taxonomy of the group. Annandale’s Fauna published in 1911,
however, includes species outside the present Indian territory.
In the present Indian prospect 30 species of freshwater sponges belonging to 10
genera, represented by a single family SpongillidaeGray, 1867, are known to be present (Soota1991). This is a significant number
in that over 81% (30 species out of 37 known) of the Oriental species are found
in India.
Attempts to summarize
knowledge of Indian freshwater sponges following Annandale (1911) include
publications by Khera & Chaturvedi(1976) and Soota (1991). A few reports and checklists
concerning freshwater sponges from different regions of India have also been
published (Tonapi 1964; Rao& Khan 1982; Soota & Pattanayak1982; Patil 1986; Dutta et
al. 2000; Devarshi 2006; Patil& Talmale 2007; Kakavipure& Yeragi 2008), however, detailed work on the
biology or systematics of Indian freshwater sponges using modern techniques
like scanning electron microscopy (SEM) imaging and DNA barcoding has not been
attempted. In this paper we provide
views of internal morphology of gemmules using SEM
because gemmule structure is considered
diagnostic. We feel there is a need
for fresh surveys and collection since freshwater habitats are rapidly
undergoing drastic modification.
Materials and Methods
Sponges were collected from
two freshwater habitats from Pune City. (i) A temporary freshwater pool near Dighi, Alandi Road, Pune (Image
1). This water body is about 50m in
diameter. The maximum known depth
is about 6–8 m. There are few
submerged and emergent aquatic plants and much of the bottom is rocky. There is no major water-body in the
vicinity; (ii) A man-made permanent freshwater
habitat, Pashan Tank, Pune (Images 2&3). This tank is about 500m in
diameter. The maximum known depth
is about 12–15 m. Aquatic
plants are present in good numbers and the invertebrate fauna is also rich
(H.V. Ghate unpublished). It is one of the favouritedestinations for birds migrating in winter. Anthropogenic activities, like swimming,
fishing are a regular practice. Pashan tank was constructed as a permanent freshwater
reservoir to supply potable water.
Sponges were visually located
in the peripheral shallow margins of the water-bodies. Surveys were also conducted in dry
seasons so as to collect the dry sponges (with large number of gemmules), exposed due to receded water levels. Sponges were photographed in field with
a digital camera (Canon PowerShot A2100 IS) and then
scraped off from the substrata using a scalpel. Dried sponges were kept without any
chemical treatment; small pieces of live sponges were either fixed and
preserved in 70% ethanol or simply dried.
For light microscopic
observations, permanent preparations were made of body as well as gemmule-spicules for all species by boiling small pieces of
sponge in concentrated HNO3, dehydrating through pure ethanol and
finally mounting in synthetic medium DistyrenePlasticizer Xylene (DPX, Qualigen).
For scanning electron
microscopic (SEM) investigation, spicules isolated with above method were
thoroughly washed with absolute ethanol and dried. Gemmules from
dry preserved sponges were soaked in 70% ethanol overnight, in order to avoid
the preservation artefacts in morphology. A clean cut, passing right through the
foramen and foraminal tube of the gemmule,
was made using a sharp blade. Both intact as well as cut gemmuleswere treated with absolute ethanol and then treated with hexamethyldisilazane(HMDS, SRL Chemicals), and allowed to dry, as per the protocol given by Nation
(1983). Processed spicules and gemmules were mounted on the SEM stub with the help of
double sided carbon-tape. Samples were sputter-coated with platinum (at
thickness of about 15nm) and scanned and photographed using Analytical SEM
(JEOL JSM - 6360 A) with voltage at 10kV and 20kV for spicules and gemmules, respectively. Digital SEM images were cleaned and
adjusted for contrast using Adobe Photoshop CS5 Version 12.0.
Determination of species was
based on careful microscopic examinations of these permanent preparations and
with the help of keys and diagrams given by Annandale (1911) alone while their
recent nomenclatural status was checked with Penney & Racek(1968). No other original papers on
sponge taxonomy were consulted to identify a species. References for the authority of various
genera and species as well as synonyms, which are usually cited only in
taxonomic large treatises, and are cited by Annandale (1911) and Penney & Racek (1968), are therefore not cited again in this
communication. Readers are
therefore referred to these original works for additional information. Morphometry of
various kinds of spicules and gemmules was performed
by using stage and ocular micrometer and by analysingthe SEM images with Adobe Photoshop. For measurement, 10 gemmules and spicules of
each type were analyzed under light microscope. As far as possible, while describing our
material we have used the standard terminology provided by Boury-Esnault& Ruetzler (1997).
Observations and Comments
We provide additional
information about five species of freshwater sponges, viz., Dosilia plumosa (Carter, 1849); Radiospongilla cerebellata (Bowerbank,
1863); Ephydatia meyeni(Carter, 1849); Eunapius carteri (Bowerbank, 1863) andCorvospongilla lapidosa(Annandale, 1908), all belonging to Spongillina: Spongillidae, collected from Pune. We provide habitus photographs and SEM
images of body as well as gemmule-spicules and gemmules for all species for the first time in the sense
that no Indian work has given this information before, although a few species have been investigated by foreign workers using SEM. For each species we are giving brief
comments on habitus and also diagnostic characters with respect to spicules andgemmules.
Dosilia plumosa (Carter, 1849)
Genus DosiliaGray, 1867 (type species: Spongilla plumosa Carter, 1849), according to a recent revision
by Cândido et al. (2010), includes five species and D.plumosa is the only member known from Indian
freshwaters (Annandale 1911; Penney & Racek 1968;Soota 1991; Manconi & Pronzato 2002; Cândido et al.
2010).
Dosilia plumosa were collected in November
1998 from a temporary freshwater pool located near Dighi,Alandi road, Pune (collector H.V. Ghate)
(Image 1).
Habitus: The sponge was found attached to submerged plants as well as
rocks in shallow regions of the pond (maximum depth about 30cm). Live sponge is often bright green in colour and exhibits typically lobed and irregular body
shape; oscula are small. Body is moderate in size, soft and often
very fragile.
Diagnostic characters
Spicules (Images 4A–C): Megascleres are feebly curved, slender,
entirely smooth oxeas (311–541 x 14–18
µm) (Image 4A). Microscleresare euasters chiefly nodular at centrewith 10–12 rays radiating in all directions; rays generally with smooth
shaft but bearing minute recurved spines in terminal
regions (diameter: 25–39 µm) (Image 4B). Another kind of microslere, though sparsely present, is microspined oxeas with 2–4 rays projecting from the central
region (39–68 x 18–29 µm) (Image 4C). Gemmuloscleresare typically birotulate with straight, slender and
entirely spined shaft with distinctly umbonate rotules having dentate
margins; rotules on both ends of the shaft are almost
identical in morphology (75–93 x 3–4 µm; diameter of rotules: 18–25 µm) (Image 4B&C).
Gemmules (Images 4D–G): Gemmuleswere profusely found throughout the skeletal meshwork of the dried sponge. They are somewhat ovoid in shape
(diameter: 700–850 µm) and a little depression is seen in dried
specimen. Pneumatic layer is well
developed and granular. Shafts of
the gemmuloscleres are embedded perpendicularly in
the pneumatic layer, whereas the rotules are left
exposed on either sides of it (Images 4E&F). Foraminal tube
is distinctly seen, straight but short (Images 4E&G).
Radiospongilla cerebellata (Bowerbank,
1863)
Genus RadiospongillaPenney & Racek, 1968 (type species: Spongilla sceptroides Haswell, 1882), is one of the large genera of Spongillidae. Manconi & Pronzato (2002)
have summarised 15 species under this genus. Osborn et al. (2008) have described a
new species, Radiospongilla pedderensis from Pedder Lake,
Tasmania. Six species have been
reported from India: R. cantonensis (Gee,
1929); R. cinerea (Carter, 1849); R. crateriformis (Potts, 1882); R. hemephydatia (Annandale, 1909); R. indica (Annandale,
1907); and R. cerebellata (Annandale 1911;
Penney & Racek 1968; Soota1991; Manconi & Pronzato2002).
Radiospongilla cerebellata were found in December 2011
in the same ephemeral freshwater pool near Dighi, Alandi Road, Pune (collector S.S. Jakhalekar) (Image 1). Spicules and gemmules from this species
matched with those of Spongilla reticulata as described by Annandale (1907);
subsequently treated as Spongilla lacustris reticulata by
Annandale (1911). This species is
now treated as synonym of R. cerebellata (Bowerbank), as per Penney & Racek(1968).
Habitus (Images 5&6): Radiospongilla cerebellata were found attached to submerged vegetation and rocks (Image 5).
Sponge often forming thin, flat cushion-like structures on surfaces of rocks;
sometimes moderately large individuals (diameter: 8–10 cm) with distinct
surface studded with finger-like projections. Surface shows body spicules radiating
out perpendicularly and the oscula are conspicuous
(Image 6). Colourin living state varies between off-white to green. Overall consistency of the sponge is
loose and fragile.
Diagnostic characters
Spicules (Images 7A&B): Megascleres are
moderately stout, straight or feebly curved entirely smooth oxeas(344–410 x 11–14 µm) (Image 7A). Microscleresare absent. Gemmuloscleresare rarely straight otherwise usually curved strongyles,
covered with many small spines, the spines in the middle are erect and often
subdivided whereas those on the extreme ends curve back towards the middle
region (71–89 x 3–4 µm) (Image 7B).
Gemmules (Image 7C–E): Numerous gemmuleswere found throughout the skeleton of dried sponge. These are also formed in projecting
finger-like processes, thereby increasing the chances of dispersal in space, as
the projections are more likely to break apart and carried away with wind or
water currents. Gemmulesare spherical in shape (diameter: 500–625 µm) (Images 7D&E). These possess a relatively thick
pneumatic layer with minute air-spaces in the
periphery. Gemmuloscleresare inserted in this layer in two distinct layers (Image 7E). The inner layer is formed by radially
arranged gemmuloscleres, which are not exposed to
outside. The outer layer, lying
just above the inner one, is composed of horizontally or tangentially arranged gemmuloscleres with one of the ends inserted in the
pneumatic layer and the other one projecting outside. The foramen is drawn into a slender,
straight and comparatively longer foraminal tube which travels its way throughout the extraordinarily
thick pneumatic layer (Images 7C&E).
Ephydatia meyeni (Carter, 1849)
Genus Ephydatia Lamouroux, 1816 (type species: Spongia fluviatilis Linnaeus, 1759), consists of nine
species (Manconi & Pronzato2002). Only two species are known
from India: E. fluviatilis inhabits
northeastern India, whereas E. meyeni is found
throughout India (Annandale 1911; Penney & Racek1968; Soota 1991; Manconi& Pronzato 2002).
Ephydatia meyeni were collected in June 1997
from a man-made freshwater reservoir located at Pashan,
Pune (collector H.V. Ghate) (Images 2&3).
Habitus (Images 8&9): Ephydatia meyeniwas observed to be attached to rocky substrata. It formed drab yellowish-brown growths
on the submerged surfaces of rocks. Annandale (1911) describes the sponge as flat encrustations having very
little thickness. However, we observed
considerably thick and globular shapes of the sponge (Image 8). We do not know the precise reason behind
this, but the environmental conditions might have some role to play here. We have also collected a green specimen
of E. meyeni from another habitat near Satara, Maharashtra in May 2012 (Image 9). Body has inconspicuous oscula, firm consistency and is moderately hard.
Diagnostic characters
Spicules (Images 10A&B): Megascleres are
moderately stout, sharply pointed, entirely smooth oxeas(344–459 x 14–21 µm) (Image 10A). Microscleresare absent. Gemmuloscleresare birotulate, with a stout shaft and pair of
identical discs, shaft is rarely smooth or usually with 1–3 erect,
pointed spines which are perpendicular to the main axis; rotulesare discoidal with irregular and deeply notched
margins; discs are often found singly (25–39 x 4–7 µm; diameter of rotules/discs: 18–25 µm) (Images 10A&B).
Gemmules (Images 10C–E): Gemmuleswere abundant in the sponge collected in summer. These were spread throughout
the skeleton and were also seen on the surface (Images 8&9). Gemmules are
roughly spherical or sub-spherical (diameter: 325–375 µm) (Images
10D&E). Pneumatic layer is
moderately thick, granular with irregular, small air-spaces,
with gemmuloscleres embedded within it (Image
10E). Foraminaltube is not clearly visible; rather the foramen is raised slightly above the
surface giving it an inverted funnel like appearance (Image 10C).
Eunapius carteri (Bowerbank,
1863)
Genus EunapiusGray, 1867 (type species: Spongilla carteri Bowerbank, 1863), is
a relatively speciose genus with 14 known species (Manconi & Pronzato2002). E. carteri is the most widespread species in Indian
region; the other Indian species are E. calcuttanus(Annandale, 1911); E. crassissimus (Annandale,
1907) and E. geminus (Annandale, 1911)
(Annandale 1911; Penney & Racek 1968; Soota 1991; Manconi & Pronzato 2002).
Eunapius carteri were also collected from Pashan Tank, Pune in February 2012 (collector S.S. Jakhalekar) (Images 2&3).
Habitus (Images 11&12): Numerous individuals of Eunapius carteri were found attached to the rocky wall of Pashan Tank (Image 3). This sponge grows huge in size and these also outnumber any other sponge
sharing the habitat. The sponges
were roughly circular in shape and measured up to 20cm across (Image 11). Dried specimens are pale to dark yellow
(Image 11), whereas colour of live animal ranges from
yellow to green (Image 12). Surface, in life, is mostly irregular, thrown into stubby projections
with broad base and often interrupted by large osculameasuring 3–4 mm in diameter. Oscula are not raised above the surface (Image
12). Numerous tubificidworms and chironomid larvae were found living in
association with the live sponge. Dead sponge, having lost its consistency and left only with its
skeleton, offers shelter to small insects like bugs.
Diagnostic characters
Spicules (Image 13A): Megascleres are quite stout, straight
or feebly curved entirely smooth oxeas (318–364
x 14–18 µm). Microscleres are absent. Gemmuloscleresresemble megascleres but are much smaller in size,
slender and as a rule slightly curved and smooth oxeas(114–179 x 4–7 µm) (Image 13A).
Gemmules (Image 13B–D): Abundant and were scattered
singly throughout the skeleton of the sponge. These were unevenly distributed; densely towards the substratum and sparsely
elsewhere. These are sub-spherical
and are found in a variety of sizes (diameter: 525–600 µm). Gemmuloscleresare horizontally or tangentially arranged on the periphery of the thick
multilayered pneumatic layer (Images 13C&D). Penney & Racek(1968) describe the foramen to be surrounded by a crater like depression. We did not observe any depression in our
fresh material, so it could simply be due to artefactsoccurring during preservation. Annandale (1911) finds apt resemblance between the shapes of the foraminal tube and an inverted bottle (Images 13B&D).
Corvospongilla lapidosa (Annandale, 1908)
Genus Corvospongilla Annandale, 1911 (type species:Spongilla loricata Weltner, 1895) was recently revised by Manconi & Pronzato (2004). While describing a new species of Corvospongilla from Thailand, Ruengsawanget al. (2012) have stated that the current number of species under this genus
is 19. Five species viz. C. burmanica (Kirkpatrick, 1908); C. caunteri Annandale, 1911; C. ultima Annandale, 1910; C. bhavnagarensis Soota et al., 1983 and C. lapidosaare known to be present in India. C. bhavnagarensisis a relatively recent addition to the Indian freshwater sponge fauna (Soota et al. 1983; Soota 1991) .
This species however has apparently been not taken into the account while
compiling all freshwater sponges under a single suborder: Spongillinaby Manconi & Pronzato(2002). Ruengsawanget al. (2012) have listed C. bhavnagarensisunder the Genus Corvospongilla and World Porifera Database also maintains it as a valid
species. C. bhavnagarensis and C. lapidosaare endemic to Indian region (Annandale 1911; Penney & Racek1968; Soota et al. 1983; Soota1991; Manconi & Pronzato2002).
Corvospongilla lapidosa were found in the same Tank
at Pashan, Pune in February 2012 (collector S.S. Jakhalekar) (Images 2&3).
Habitus (Image 14): Enormously spread encrustations of Corvospongilla lapidosa were found on vertical surface of the
rocky wall of Pashan Tank (Image 3). Maximum length of the encrusted growth
recorded is nearly 0.75m; though, thickness was never greater than 1–1.5
cm. Sponge has peculiar morphology
with colour ranging from off-white, grey to black,
perhaps due to trapped silt (Image 14). The surface is corrugated and studded with vertical spiny spicular projections, thus called ‘hispid’ (Boury-Esnault & Ruetzler1997). Osculaare small, not more than 1mm in diameter, thus inconspicuous. Consistency is hard due to thick
skeletal meshwork formed by large number of spicules.
Diagnostic characters
Spicules (Image 15A-C): Megascleres are stout, nearly straight
or feebly curved, entirely smooth strongyles(189–250 x 18–21 µm) (Image 15A). Microscleres,
scarcely present, are micro-birotulates with slender,
smooth shaft ending in 6–8 recurved spines
giving it an appearance similar to the umbonate,
deeply notched rotule (18–36 x 2–4 µm; diameter
of rotules: 7–13 µm) (Image 15C). Gemmuloscleresare stout, straight or slightly curved strongyles,
covered with blunt microspines, some of which are
slightly back-curved, spine at the tip is broad and straight (21–39 x
5–9 µm) (Image 15B).
Gemmules (Image 15D&E): Gemmulenumber was extremely large and all gemmules were
concentrated at the base of the sponge. Region towards surface showed no gemmules. Gemmules are about
spherical or sub-spherical in shape (diameter: 725–1000 µm) (Images
15D&E). The pneumatic layer is
poorly developed having irregular consistency and very small air-spaces(Image 15E). Annandale (1911) and
Penney & Racek (1968) describe the gemmuloscleres to be attached to the outside of gemmule in mosaic pattern. We, however, could not observe such
pattern of arrangement in our specimen. Foramen was just raised above the surface.
Discussion
These five species of
freshwater sponges are known from India (Annandale 1911; Penney & Racek 1968; Soota 1991) and
Maharashtra as well (Patil & Talmale2007). Tonapi(1964), however, had missed D. plumosa and R.cerebellata in his short-note on freshwater
sponges of Pune. This paper makes
an addition of these two species to the checklist of freshwater sponges of Pune
City. Besides, this is probably the
first photographic documentation of spicules under SEM as well as photos of
habitus of each sponge reported here, as we are not aware of any similar
publication by earlier Indian workers.
The structure of spicules
(from body as well as from gemmules) conforms to that
described by earlier workers like Annandale (1911) and Penney & Racek (1968). Measurement of spicules shows that these spicules are within the
size-range published earlier (Penney & Racek1968). Gemmule structure, especially seen
in cross section with SEM, has not been mentioned by earlier Indian researchersthough Manconi & Pronzato(2002) have studied some of these sponges with SEM. In a detailed paper on gemmule structure, and the role of gemmulesin dispersal and natural history of freshwater sponges, Manconi& Pronzato (2007) have emphasized that cryptobiosis associated with gemmuleshas played a significant role as an efficient device for survival under dry
conditions and subsequent dispersal. These authors have also discussed the role of pneumatic layer and
spicule-layers in the wall or theca of the gemmulesand other aspects of gemmule structure; these layers
have been shown in our species and their structure matches with the description
given by Manconi & Pronzato(2007). We have observed that there
are literally thousands of gemmules with even a
moderate sized (diameter about 15cm) Ephydatia meyeni and we can imagine how many potential new
sponges can arise from these gemmules, as these
spread away from dry sponge. Certainly this capacity to produce vast number of gemmules which can resist dry
period must have helped freshwater sponges to survive and disperse in a
vast number of suitable habitats all over the world.
We now feel that modern
techniques like SEM imaging or DNA based phylogeny must be employed to solve
the intricacies of taxonomy/phylogeny of freshwater sponge in India. Fresh
collection revealing new localities and careful biological observations are
very much needed for the establishment of sound taxonomic base for freshwater
sponges in India. This is necessary
because Manconi & Pronzato(2007) are of the opinion that the present number of species of freshwater
sponges is an underestimation and that additional detailed work is likely to
reveal more species. More detailed
work is therefore likely to reveal additional species of freshwater sponges.
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