Status , abundance and habitat preference of butterflies ( Insecta : Lepidoptera ) in Chittagong University Campus , Chittagong , Bangladesh

A study was conducted on the butterflies of the Chittagong University Campus (CUC), Bangladesh between March 2014 and May 2015. A total of 142 species of butterflies belonging to 87 genera and six families (Hesperiidae, Papilionidae, Pieridae, Lycaenidae, Riodinidae and Nymphalidae) were recorded from the CUC during the study period. Family Nymphalidae comprised the highest number of species followed by Lycaenidae, Hesperiidae, Pieridae, Papilionidae and Riodinidae. The abundance of this species stated in terms of very common, common, uncommon, rare and very rare. The butterflies used different types of habitat (viz., grass land, crop land, open forest, scrub forest, dense forest and bamboo patch) and among all, scrub forests were the most preferred habitat. Mud puddling of 35 species were also observed. Five species of butterflies (Ampittia dioscorides, Matapa purpurascens, Polytremis eltola, Unkana ambasa and Ypthima ceylonica) were recorded for the first time in Bangladesh. ey ords Abundance, Bangladesh, butterfly, Chittagong University Campus, habitat, status. Butterflies are beautiful flying creatures of nature. Since the early 18th Century, 19,238 species of butterflies have been documented worldwide (Heppner 1998). The exact number of extant species of butterfly is not known but there are about 16,823 species spread throughout the world (Landing 1984); of which 1,318 species have been recorded in India (Varshney & Smetacek 2015), 643 species in Nepal (Nepal Safari 2016), 247 species in Sri Lanka (van der Poorten & van der Poorten 2016), and 1,014 species in Myanmar (SST Tourism 2011). Butterflies are not well documented in Bangladesh. Baksha & Choudhury (1983, 1985) identified respectively 17 species from the family Pieridae and 16 species from Papilionidae. Larsen (2004) annotated a list of butterflies and mentioned 236 species from Bangladesh. Ahmad et al. (2009) compiled 148 species in the country. Chowdhury & Hossain (2013) listed 225 species from Bangladesh and forecast that the number of species may exceed 400. So, more studies are needed to list the total number of butterflies in the country as new records are being added periodically. In Bangladesh, some regional checklists have also been prepared. For instance, Khan (2001) accounted for 49 species from Tangail District; Chowdhury & Mohiuddin (2003) reported 121 species from the eastern border (Sylhet and Moulvi Bazar districts of

Butterflies are beautiful flying creatures of nature.Since the early 18 th Century, 19,238 species of butterflies have been documented worldwide (Heppner 1998).The exact number of extant species of butterfly is not known but there are about 16,823 species spread throughout the world (Landing 1984); of which 1,318 species have been recorded in India (Varshney & Smetacek 2015), 643 species in Nepal (Nepal Safari 2016), 247 species in Sri Lanka (van der Poorten & van der Poorten 2016), and 1,014 species in Myanmar (SST Tourism 2011).
Butterflies are not well documented in Bangladesh.Baksha & Choudhury (1983, 1985) identified respectively 17 species from the family Pieridae and 16 species from Papilionidae.Larsen ( 2004) annotated a list of butterflies and mentioned 236 species from Bangladesh.Ahmad et al. (2009) compiled 148 species in the country.Chowdhury & Hossain (2013) listed 225 species from Bangladesh and forecast that the number of species may exceed 400.So, more studies are needed to list the total number of butterflies in the country as new records are being added periodically.
In Bangladesh, some regional checklists have also been prepared.For instance, Khan (2001)  Alam & Ullah (1995) reported 22 species of butterfly from the Chittagong University Campus (CUC) and since their publication, no further studies have been conducted.Hence, it is needed to study the butterfly fauna of the CUC, which may also enrich the checklist of the country.On the other hand, status, abundance and types of habitat used by butterflies are useful to document in order to conserve these creatures and their habitats, which play a significant role in the ecosystem.Hence this study was designed with the major aims to know: (i) status of the recorded species in the CUC, (ii) abundance of occurrence of the butterfly families, and (iii) the species-wise habitat preference in the area.

tudy rea
The CUC (Fig. 1) is situated at Zubra Village under Fatehpur Union Parishad of Hathazari Upazila (subdistrict) in Chittagong District, Bangladesh (22 0 27'30"-22 0 29'0"N & 91 0 46'30"-91 0 47'45"E).It is about 22km north of Chittagong City, 3km southwest of Hathazari Upazila headquarters and about 6km east from the Bay of Bengal.The CUC is surrounded by hills of the Chittagong hill region and bisected by a small stream.It is a large area compared to the other universities of the country comprising 710ha (1,754 acres) of land.The diversity of plants and animals is quite rich in the CUC compared to other universities of Bangladesh.It is covered with about 72% hills, lakes, ponds and plain lands and valleys are 15.9m above sea level (Islam et al. 1979).The soils of all profiles are characterized by coarse texture (38-73 % sand fraction), high bulk density (1.15-1.32mgmˉ³), low organic-C content (0.26-1.73 %), and acid soil reaction (pH H2O varied from 4.44 to 5.52 and pH KCl from 3.57 to 4.90) (Akhtaruzzaman et al. 2014).
There are three seasons in the CUC like elsewhere in Bangladesh (Ahmad 1968): Summer (March-May), Monsoon (June-October) and Winter (November-February).About 60% land area of the CUC is covered by steep and very steep hills (Hossain et al. 2013); although it is composed of hills, valleys and plains.The vegetation is semi-evergreen (Ahsan & Khanom 2005).A total of 665 plant species under 126 families and 404 genera are found in the CUC, of which 550 are dicotyledons and 115 are monocotyledons (Alam & Pasha 1999).

ield study
The study on butterflies was conducted in the CUC for 15 months between March 2014 and May 2015.A total of 60 days' observation was done during this study period.Field observations were done throughout the day but emphasis was given to bright sunny periods of the day when butterflies are more active.Opportunistic records of butterflies have also been included in the list.The whole study area was divided into six sites for convenience of the study (Fig. 1).Different types of habitat used by butterflies (viz., grass land, crop land, open forest: composed of trees, shrubs and grasses with discontinuous canopy, scrub forest: shrub land and bushy jungles, dense forest and bamboo patches) have also been recorded during the study.
Butterflies were surveyed through the existing roads, trails, streams and bridle paths for a whole day, once a week and covering all the sites of the CUC in a cyclic order in each month.pe ies den a on During surveys for butterflies, the species were recorded in a notebook and cryptic specimens were photographed using cameras (Canon EOS 600D with 75-300 mm IS II lens and Canon EOS 60D with 300mm prime lens) for confirming the species identities.Collection and killing of the specimens were avoided.The individuals were identified following the keys developed by Marshall & de Niceville (1883), Bingham (1905), Evans (1932) and Bashar (2014); and field guides (e.g., Chowdhury & Hossain 2013;Kehimkar 2013).In this study, butterflies were assessed as very common (vc), common (c), uncommon (uc), rare (r) and very rare (vr) (Table 1).
One-hundred-and-forty-two species of butterflies belonging to 87 genera and six families (  3).Of the recorded species, 16.9% were very common, 22.5% common, 20.4% uncommon, 19.7% rare and 20.4% were very rare.Most of very common and common species belonged to the family Nymphalidae, uncommon to Lycaenidae, rare species to Hesperiidae and very rare species belonged to Hesperiidae and Lycaenidae (Table 3).
The CUC is an ecotone area connecting hilly forest with plain land and comprises grassland, cropland, open forest, scrub forest, dense forest, bamboo patch and hill streams for mud puddling.Hence, the butterflies get opportunities to use different types of habitat in the CUC (Figs. 2 & 3).Thirty-five (24.6%) of 142 species of butterflies used all kinds of habitat.Eighty-one species preferred multiple habitats.Out of these 81 species, one species (Pachliopta hector: Papilionidae) preferred five types of habitat, eight species (5.6%) chose four types, 27 species (19%) used three types, 44 species (31%) favored two types and the rest 27 species (19%) were observed only in a single habitat.
Mud puddling was observed in 35 species (Fig. 3), which comprised five families; and among them Lycaenidae accounted the highest number of species (15 species, 42.9%) and Hesperiidae the lowest number (2 species, 5.7%).

Butterflies in Chittagong University Campus Haidar et al.
accounted for 49 species from Tangail District; Chowdhury & Mohiuddin (2003) reported 121 species from the eastern border (Sylhet and Moulvi Bazar districts of Sylhet division, and Chittagong and four hill districts in Chittagong division).Hossain et al. (2003) recorded 51 species from Jahangirnagar University Campus.Khandokar et al. (2014) recorded 160 species from Lawachara National Park, Moulavibazar.