A rare
agaric (Agaricomycetes: Agaricaceae) from a sacred grove of Eastern Ghats,
India
M. Kumar 1 & V.
Kaviyarasan 2
1,2 Lab No. 404, CAS in Botany, University of
Madras, Guindy Campus, Chennai, Tamil Nadu 600025, India
Email: 1 mycologykumar@gmail.com (corresponding
author)
Date of publication (online): 26 May 2011
Date of publication (print): 26 May 2011
ISSN 0974-7907 (online) | 0974-7893 (print)
Editor: V.B. Hosagoudar
Manuscript details:
Ms
# o2626
Received
13 November 2010
Final
received 22 April 2011
Finally
accepted 02 May 2011
Citation: Kumar, M. & V. Kaviyarasan (2011). A rare
agaric (Agaricomycetes: Agaricaceae) from a sacred grove of Eastern Ghats,
India. Journal of Threatened Taxa 3(5):
1778–1781.
Copyright: © M. Kumar & V. Kaviyarasan 2011. Creative
Commons Attribution 3.0 Unported License. JoTT allows unrestricted use
of this article in any medium for non-profit purposes, reproduction and
distribution by providing adequate credit to the authors and the source of
publication.
Acknowledgements: The authors thank the University Grants Commission for the
financial assistance, Prof. T. Vasantha Kumaran, former Head, Department of
Geography for guiding us to the collection spot. We also express our gratitude to Prof. N. Anand, former Director
and Prof. R. Rengasamy, Director CAS in Botany, University of Madras for
providing lab facility.
For figures, images -- click here
Clarkeinda trachodes (Berk.) Singer,is a rare tropical Asian monotypic agaric (Leelavathy et al. 1981; Singer 1986;
Pegler 1986; Zhu-Liang 1991; Carmine & Contu 2002) belonging to the family
Agaricaceae. It is a large
lepiotoid agaric, characterized by the presence of volva and annulus. The spore print is olive brown and the
spores are small with truncated germ pore. This species has been reported earlier from only six places
around the globe—Sri Lanka (Pegler 1986), India (Leelavathy et al. 1981),
Malaysia (Pegler 1986), Indonesia, China (Zhu-Liang 1991) and Italy (Carmine
& Contu 2002). This forms the second report of this species from India
indicating that this species is well represented in southern India.
It is similar to Chlorophyllum but with marked
differences: the basidiocarp with a large plate like fawn coloured scales in
the centre (mostly star shaped) of the pileus, dextrinoid spores with a compound
wall and presence of volva. Matured spores exhibit metachromatism in cresyl blue. This specimen also shows similarity
with Macrolepiota rhacodes in having the plate like squamules on the
surface of the cap but differs in the absence of bulbous base and presence of
volva.
Sacred groves are patches
of natural vegetation surviving in man-modified landscapes
which are dedicated to local deities and protected by religious tenets
and cultural traditions, with religious fervor. They are a social institution, which permit management of
biotic resources through participation of people of the local community. Although the practice of conserving the
local biodiversity through sacred groves is very old, the importance has been
acknowledged only recently (Ramanujam & Cyril 2003). In India, during 1997, the existence of
thousands of such sacred groves was recorded along the plains and hills of the
Indian subcontinent and confirmed their floristic richness confined within
islets of diverse habitats (Ramakrishnan et al. 1998). They are a rich repository of
biodiversity, a myriad of valuable ecosystem services and serve as ideal study
sites to address many ecological issues related to forest ecosystem dynamics
and management (Tripathi 2005). InTamil Nadu, there are around 503 sacred groves (Anthwal et al. 2006). In most localities, sacred groves are
being increasingly exposed to various kinds of threats leading to either
qualitative degradation or total disappearance. There have been numerous studies on medicinal herbs,
diversity of plants and insects, ecology and anthropology (Gadgil & Vartak
1976; Tripathi 2005). However, studies on the diversity of mushrooms in such sacred groves islacking.
As a part of systematic
studies on the agarics of southern India, Kolli Hills were studied for the past
four years (March 2006 – August 2010). Kolli Hills are the part of Eastern Ghats, which is one of
the richest floristic areas in the world (Bhusan 2003) with hills rising from
200–1415 m with deep ravines and high peaks (Chittibabu &
Parthasarathy 2000; Ramachandran et al. 2007), having a wide range of
ecosystems and species diversity, located at the tail end of the Eastern Ghats
in Namakkal District in the state of Tamil Nadu (Fig. 1). They are part of the Talaghat stretch.
Kolli Hills are known for sacred groves and other ritual practices (Mulligan et
al. 2007).
Materials and Methods
Collections were made as
suggested by Largent (1977) and Atri et al. (2003). Macroscopic details such as
shape, colour, dimension and odour of fresh basidiocarps were recorded. Terminologies used by Largent were
followed for recording the characters of specimens. Kornerup & Wanscher’s (1978) colour chart was followed
to determine colour of the fresh specimens. The specimens were dried by using a mushroom drier, labeled
and preserved in sealed polythene covers along with naphthalene balls in order
to safeguard them from insects pests. The specimens were deposited in the
Herbarium of Madras University Botany Laboratories (MUBL) for future studies
(MUBL No. 3673).
The dried specimens were
revived in 3% KOH. Stains such as
1% aqueous Phloxine, Acetocarmine and Melzer’s reagent were used to study
carminophilous reaction, amyloidity reaction of the spores (Largent 1977). Spore print was taken and spores,
basidia, cystidia, hyphae, etc., measured using micrometric techniques. Line
diagrams were drawn with the aid of camera lucida attachment (Fig. 2).
Taxonomy
Material examined:
22.v.2005, large fruit bodies (12 nos) of Clarkeinda trachodes were found to occur on
soil in solitary as well as in troops in the shady places of sacred groves in
Nariankadu of Kolli Hills (Nammakkal District), Tamil Nadu (Image 1 a,b). coll. M. Kumar &
V.Kaviyarasan, (MUBL. No. 3673).
The culture of C. trachodes (VKMK06) is maintained in
MUBL Culture Collection Centre.
Macroscopic
description
Basidiocarp troops,
terrestrial in grass land. Pileus 7.5–13 cm, broadly parabolic to convex
or almost applanate to slightly uplifted; surface pale (4A1–4A2) with
greyish-brown to clay squamules all over and brown plate like squamules (7E6–7F6)
at disc on a white background; dry, squamose margin striate, rimose,
appendiculate.
Lamellae free, sulphine
yellow (3A2) when young and yellowish-grey (3B2) when old, up to 12mm wide,
crowded with lamellulae of five lengths; edge thick
and not smooth.
Stipe 8.5–9.2 ×
1.4–2.2 cm, central, obclavate to cylindric, tapering upwards, with a
bulbous base, hollow, surface white, bruising to yellowish-green (4A2–4B2),
smooth.
Annulus
persistent, complex, thick, very broad, pendulous, with squamules on the bottom
of the annulus, white. Volva present, 1–3.5 cm deep, white,
lobes usually closely appressed with stipe and in some specimen inconspicuous.
Context upto 1.3cm thick at
the disc, white, changing to brownish-red when exposed, consisting of slightly
interwoven, thin walled, hyaline hyphae of 2–7.63 µm diam., inflated upto
13µm diam. Spore print ‘pale yellow’(1A3) to greyish-yellow (1B3).
Microscopic
description
Spores 5.67–8.50 ×
4.36–5.45 (7.08 ± 0.49 × 4.9 ± 0.43) µm, Q = 1.44, ellipsoid to
ovate, truncate at the apex by a broad distinct, germ pore, hyaline,
dextrinoid, not metachromatic with cresyl blue, smooth, thick complex wall.
Basidia 17.45–21.82 × 4.90–8.72 µm, clavate, bearing four
sterigmata, sterigmata short. Lamellar edge not sterile. Cheilocystidia not
observed. Pleurocystidia 21.82–22.91
× 6.54–6.89 µm, not much broadly mucronate. Hymenophoral trama
regular, 22.54–50.72 µm dia., hyaline, consisting of thin walled hyphae,
2–7 µm diam., inflated upto 15µm dia. Subhymenial layer well developed, 7–10
µm in dia., pseudoparenchymatous. Pileipellis pellicle formed
by agglutinated trichodermial hyphae. Pileal surface a trichodermial
palisade of thin walled erect hyphae, 6.76–15.49 µm dia., hyaline,
terminal elements cylindrical. Clamp connections absent in all hyphae.
The species collected from
Kolli Hills was compared with the previous Indian record of Leelavathy et al.
(1981) and found that there is not much difference in all the macroscopic and
microscopic characters except for a few slight variations like the smaller
basidia.
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