Rediscovery of the threatened Western Ghats endemic
sisorid catfish Glyptothorax
poonaensis (Teleostei:
Siluriformes: Sisoridae)
Neelesh
Dahanukar 1, Manawa Diwekar 2 & Mandar Paingankar3
1,2 Indian
Institute of Science Education and Research, Sai Trinity, Garware Circle, Pune,
Maharashtra 411021, India
3 Department
of Zoology, University of Pune, Ganeshkhind, Pune, Maharashtra 411007, India
Email: 1n.dahanukar@iiserpune.ac.in
(corresponding author), 2 manawa.d@iiserpune.ac.in, 3 mandarpaingankar@gmail.com
Date of publication (online): 26
July 2011
Date of publication (print): 26
July 2011
ISSN 0974-7907 (online) |
0974-7893 (print)
Editor: Heok Hee Ng
Manuscript details:
Ms # o2663
Received 30 December 2010
Final received 08 July 2011
Finally accepted 11 July 2011
Citation: Dahanukar, N., M. Diwekar & M. Paingankar (2011).
Rediscovery of the threatened Western Ghats endemic sisorid catfish Glyptothorax
poonaensis (Teleostei: Siluriformes: Sisoridae). Journal of Threatened Taxa 3(7): 1885–1898.
Copyright: © Neelesh Dahanukar, Manawa
Diwekar & Mandar Paingankar 2011. Creative Commons Attribution 3.0 Unported
License. JoTT allows unrestricted use of this article in any medium for
non-profit purposes, reproduction and distribution by providing adequate credit
to the authors and the source of publication.
Author Detail: Neelesh
Dahanukar works in ecology and evolutionary biology with an emphasis on
statistical and mathematical analysis. Manawa Diwekar is a
molecular biologist with special interests in understanding molecular
evolution. Mandar Paingankar is a molecular biologist and works on
vector biology with an emphasis on host parasite interactions. He works on
animal ecology as a hobby.
Author Contribution: ND and MP designed the research
and performed morphometry; MD performed molecular biology work; ND and MP
analyzed the data and wrote the paper.
Acknowledgements: We are thankful to Prof. L.S.
Shashidhara, Prof. Milind Watve and Dr. Sanjay Molur for continuous support and
encouragement. We are thankful to Dr. R.M. Sharma, Officer-in-charge, and
Shrikant Jadhav, Zoological Survey of India, Western Regional Centre, Akurdi,
Pune, for encouragement and helpful discussion. We thank two anonymous referees
for comments on an earlier draft of the manuscript. The CEPF-funded freshwater
assessment of the Western Ghats encouraged us to publish this work. We
duly acknowledge the help from CEPF for publication of this article.
Abstract: Glyptothorax poonaensis Hora is an
endemic sisorid catfish of the Western Ghats of India known only from its type
locality in Mula-Mutha River, a tributary of Bhima River, at Pune. This fish has not been recorded from
its type locality for more than 70 years and it was thought to be extinct. Here we report a recently discovered
population of G. poonaensis from Indrayani River, a
tributary of Bhima River. Based on
11 specimens, we have redescribed this species along with some comments on its
taxonomy, length-weight relationship, feeding and breeding habits. We also performed molecular phylogeny
of the fish by sequencing three mitochondrial genes encoding 16S ribosomal DNA,
cytochrome b and cytochrome oxidase subunit I. Molecular analysis suggests that G.
poonaensis is nested within a lineage of Glyptothorax species from
northern and northeastern India and China. Further, our analysis reveals that southern Indian species
of Glyptothorax do not
form a monophyletic group. Molecular dating of divergence times indicates that G.
poonaensis diverged from other northern Indian species 1.9 to 2.5
million years ago. Current
knowledge suggests that the species could be found in two river basins with
total extent of around 6100km2; however, the species is already
suspected to be locally extinct from half of its known extent of occurrence. Furthermore, the habitat of the species
may be threatened by increasing pollution, deforestation leading to siltation,
halting of flow by damming, sandmining and introduced fish species. In the light
of biodiversity conservation, especially in an important biodiversity hotspot
like Western Ghats, such rare and endemic species needs prioritization.
Keywords:Conservation, Glyptothorax poonaensis, molecular phylogeny, northern
Western Ghats.
This article
forms part of a special series on the Western Ghats of India, disseminating the
results of work supported by the Critical Ecosystem Partnership Fund (CEPF), a
joint initiative of l’Agence Française de Développement, Conservation
International, the Global Environment Facility, the Government of Japan, the
MacArthur Foundation and the World Bank. A fundamental goal of CEPF is to
ensure civil society is engaged in biodiversity conservation. Implementation of
the CEPF investment program in the Western Ghats is led and coordinated by the
Ashoka Trust for Research in Ecology and the Environment (ATREE).
For figures, images, tables -- click here
INTRODUCTION
The
Western Ghats of India harbor a rich diversity of freshwater fish with many
species endemic to this region (Shaji et al. 2000; Dahanukar et al. 2004). However, a major part of this diversity
is threatened by various anthropogenic activities (Dahanukar et al. 2004), and
unless serious efforts are taken to conserve natural resources, it is possible
that rare and endemic species in this biodiversity hotspot may go extinct in
the near future. This is
especially true for stenotopic fish species like the ones in the genus Glyptothorax,
which prefer clear hill streams with rapid currents. Globally, Glyptothoraxis represented by more than 70 species (Anganthoibi & Vishwanath
2010). In the Western Ghats, Glyptothoraxis currently represented by 10 species (Gopi 2010), namely G. anamalaiensis Silas,G. annandaleiHora, G.
davissinghi Manimekalan & Das, G. houseiHerre, G.
kudremukhensis Gopi, G. lonah (Sykes), G. madraspatanum(Day), G.
malabarensis Gopi, G. poonaensis Hora, and G. trewavasaeHora. Out of these species, G. poonaensisis considered to be extinct from its type locality (Kharat et al. 2003).
Hora
(1938) described G.
poonaensis as a subspecies of G. conirostre,
from the Mula-Mutha River, a tributary of Bhima River in the northern Western
Ghats, India. The description was
based on five specimens, one of which was collected by A.G.L. Fraser during
1936–1937 from Kharadigaon area of Mula-Mutha River (Hora 1938; Fraser
1942). Since then at least three
independent studies were carried out on the freshwater fish fauna of Mula-Mutha
Rivers by Tonapi & Mulherkar (1963), Wagh & Ghate (2003; this work was
actually carried out during 1992–1995) and Kharat et al. (2003) and this
species has never been encountered. Studies on other nearby rivers, namely Pavana River (Chandanshive et al.
2007) and Indrayani River (Yazdani & Mahabal 1976), which are tributaries
of Mula-Mutha and Bhima rivers respectively, did not record this species
either. In an extensive survey of
Krishna River system, Jayaram (1995) did not record this species but noted that
it had been recently recorded by Ghate et al. (1992). However, Ghate et al. (1992) did not base their record on
material they collected, but on records in earlier literature. Glyptothorax poonaensisis also mentioned in the checklist of fish from Pune District by Tilak & Tiwari
(1976) but it remains unclear whether this record is based on actual collection
or the existing literature. Similarly, Yadav (2003) recorded this species from the collection of
Western Regional Station, Zoological Survey of India, Pune, during 1960–1995,
without mentioning collection localities or dates of collection. As a result,
there are no reliable records of G. poonaensis since its original
description in 1938. This is the
reason Kharat et al. (2003) considered G. poonaensis to be possibly extinct
in the Mula-Mutha River after a detailed survey of the river drainage.
In
a resent survey of Indrayani River, also a tributary of Bhima River (and very
close to the Mula-Mutha River of Pune; approximately 18km by land but around
120km river stretch from the type locality of the fish) we discovered a
population of G.
poonaensis. In this paper we redescribe the species with further details on its
morphomety, molecular phylogeny, current distribution, length-weight
relationship, feeding habits, breeding habit and threats to the species. This data will be helpful while
designing and implementing conservation strategies.
METHODS
Study area and specimen collection
The Indrayani River is a
tributary of Bhima River, which is itself a major tributary of the Krishna
River system. In our study on the
fish diversity of Indrayani River, specimens of Glyptothoraxwere collected at Markal (18.6710N, & 73.9810E) from
June to August 2010 from the local fishermen. The specimens were preserved in 4% formaldehyde and were
identified based on available literature (Hora 1938; Silas 1951; Talwar &
Jhingran 1991; Jayaram 2010). Four
specimens from our study are deposited in the collection of Wildlife
Information Liaison Development, Coimbatore under the accession numbers WILD-11-PIS-004,
WILD-11-PIS-005, WILD-11-PIS-006 and WILD-11-PIS-018. Three specimens from our collection are deposited in the
Zoological Survey of India, Western Regional Centre, Akurdi, Pune under the
accession numbers ZSI Pune P/2431, P/2432 and P/2433.
Biological and morphological data collection and
analysis
Morphometric and
meristic data were collected following Vishwanath & Linthoingambi (2005),
Ng & Kottelat (2008) and Jayaram (2010). Wherever there was discrepancy in the definition of a morphometric
character, for example body depth at anus (Ng & Kottelat 2008) versus body
depth at dorsal origin (Jayaram 2010), we took both the measurements and
recorded them appropriately. In
all 40 morphometric characters and four meristic characters were considered. Morphometry was performed using dial
calipers with a least count of 0.0254mm. We also extracted morphometric data for G. poonaensis,G. conirostre, G. lonahand G.
trewavasae from Hora (1938) for comparison. To avoid personal measurement biases
while comparing Hora’s (1938) data and ours we performed cluster analysis based
on the mean values of size adjusted morphometric data. We calculated Euclidian distances and
we used Ward’s method of clustering. Weight of each individual was measured on an electronic weighing balance
with a least count of 0.01g. We
plotted log-log plot of length and weight of the fish to determine the exponent
of the length-weight relationship given by the equation W = a Lb,
where ‘W’ is the weight, ‘a’ is the normalization constant, ‘L’ is the length
and ‘b’ is the scaling exponent. The null hypothesis that ‘b=3’ was tested by comparing the 95%
confidence interval of calculated value of ‘b’ (Quinn II & Deriso 1999,
pp.131). We dissected three
specimens in our collection to extract their gut contents and analyzed their
feeding habits.
DNA isolation and molecular phylogeny
Muscle tissues were
harvested from two fresh specimens (WILD-11-PIS-018 and ZSI Pune P/2431) and
were preserved in absolute ethanol. The tissue was digested at 500C for two hours using the STE
buffer (0.1M NaCl, 0.05 M Tris-HCl, 0.01M EDTA, 1%SDS) with 15µl proteinase K
(20mg/ml) per 500µl of STE buffer. DNA was extracted using conventional phenol-chloroform method and re-suspended
in nuclease free water. Polymerase
chain reaction was performed to amplify three fragments of mitochondrial genes
namely cytochrome b (cyt-b), cytochrome oxidase subunit I (cox1) and 16S
ribosomal DNA (16S rDNA). Primers
used for the amplification are given in the Table 1. PCR was performed in a 50µl reaction volume containing 5µl
of template DNA (~200ng), 5µl of 10x reaction buffer (100mM Tris pH 9.0, 500mM
KCl, 15mM MgCl2, 0.1% gelatin), 2µl of 25mM MgCl2, 2µl of
10mM dNTPs, 1µl of each primer, 1µl taq polymerase and 33µl distilled
water. The genes were amplified
using gene-specific primers. For 16S rDNA thermal profile was 5min at 950C,
and 40 cycles of 30 seconds at 940C, 30 seconds at 610C
and 2min at 720C followed by extension of 10min at 720C. For cyt-b and cox1 thermal profile was
5min at 95°C, and 40 cycles of 30 seconds at 940C, 30 seconds at 510C
and 2min at 720C, followed by extension of 10min at 720C. Amplified DNA fragments were purified
using the ‘Promega Wizard Gel and PCR clean up’ system and sequenced. The
purified PCR products were sequenced using ABI prism 3730 sequencer (Applied
Biosystems, USA) and big dye terminator sequencing kit (ABI Prism, USA). Sequences were analyzed by BLAST tool
(Altschul et al. 1990). These
sequences have been deposited in Genbank.
We retrieved additional
sequences of other species of Glyptothoraxfrom NCBI GenBank (http://www.ncbi.nlm.nih.gov/). We used Gagata ceniaand Bagarius
yarrelli as out-groups following Peng et al.
(2006). All the sequences used for
the analysis, along with their GenBank accession number, are given in Table
2. Our total data matrix
encompasses fragments of three mitochondrial genes namely 16S rDNA, cyt-b and
cox1 with a total sequence length of 2120 bp after alignment. Sequences were
aligned using ClustalW (Thompson et al. 1994). Phylogenetic and molecular evolutionary analyses were
conducted using MEGA version 5 (Tamura et al. 2011). Best fit model for nucleotide substitution was selected
based on minimum Akaike Information Criterion (AIC) value (Posada &
Crandall 2001). We constructed
phylogenetic trees based on maximum parsimony, minimum evolution, neighbour
joining and maximum likelihood. Reliability of the phylogenetic tree was estimated using bootstrap
values run for 1000 iterations. Based
on the calibration points available on the molecular dating of Gagata ceniaand Bagarius
yarrelli (Peng et al. 2006) we performed molecular
dating using maximum likelyhood analysis based on cyt-b gene sequence. A molecular clock test was performed to
find out whether the substitution rates were uniform (Tamura et al. 2011).
RESULTS AND DISCUSSION
In spite of several
extensive surveys in the Mula-Mutha River and other rivers in the vicinity of
Pune (Tonapi & Mulherkar 1963; Yazdani & Mahabal 1976; Kharat et al.
2003; Wagh & Ghate 2003; Chandanshive et al. 2007), G. poonaensishas not been recorded after its description by Hora (1938). The only other records of the fish by
Tilak & Tiwari (1976) and Yadav (2003) could be based only on existing
literature (see Introduction). As
a result our report of G.
poonaensis is the first verifiable record since its
original description.
Taxonomic comments
We compared the
specimens in our collection with the original description in Hora (1938) and
confirmed their conspecificity. We
further confirmed the identity of G. poonaensis by comparing our
morphological data with the data for G. poonaensis, G. conirostre,G. lonahand G.
trewavasae from Hora (1938). Size adjusted morphometric data from Hora (1938) and our specimens
(Table 3) agree closely, further verifying their conspecificity. The only disagreement between our data
and that in Hora (1938) is the length of caudal peduncle. Our measurements (19.3–22.7 % SL)
are higher than those reported by Hora (16.6–17.5 % SL), however, we
cannot comment on the reasons behind this difference because of the small
sample sizes in both cases. The
dendrogram (Fig. 1) based on the morphometrical comparisons (Table 3) suggests
that not only our specimens are similar to G. poonaensis sensu stricto but G. poonaensisis significantly different from its closely related northern Indian species G. conirostreand other species, namely G.
lonah and G. trewavasae, described from the
northern Western Ghats of India.
Glyptothorax poonaensis was
originally described as a valid subspecies of G. conirostre. Menon (1999) elevated its status to the
species level without giving any rationale and the trend was followed by
Thomson & Page (2006). Jayaram
(2009, 2010) however still considers the species as a valid subspecies of G. conirostre,
while, Ferraris (2007) has considered the species as species inquirendaeowing to the fact that the species has either been treated as valid or as a
synonym of G.
conirostre. Our analysis based on the data available on G. conirostrein Hora (1938) and comparison of our material of G. poonaesiswith the original description of G. conirostre given by Steindachner
(1867) indicates that G.
poonaensis is a valid species markedly distinct from G. conirostre. Comparison of standardized characters
given in (Table 3) suggests that G. conirostre is different from G. poonaensisin the head width and lengths of different fins. As compared to Glyptothorax conirostre, G. poonensis has
lesser height of dorsal fin (15.1–18.1 % SL vs. 19.6–20.1 % SL),
length of pectoral fin (18.6–22.8 % SL vs. 24.2–24.3 % SL), length
of ventral fin (13.7–15.8 % SL vs. 16.8–18.8 % SL), length of
longest anal fin ray (13.8–18.4 % SL vs. 18.7–19.1 % SL) and length
of dorsal spine (11.1–13.4 % SL vs. 13.6–14.8 % SL). However, as compared to G. conirostre,G. poonaensishas larger head width (17.0–21.2 % SL vs. 16.5–16.8 % SL) and inter
orbital width (6.2–8.3 % SL vs. 4.7–6.0 % SL). Jayaram (2009) has separated G. poonaensisfrom G.
conirostre based on having broader head, shorter dorsal
fin and posterior insertion of pelvic fins. We therefore suggest that G. poonaensis should be considered as
a valid species distinct from G.
conirostre.
Glyptothorax poonaensiscan be differentiated from nine other species of Glyptothoraxknown from the Western Ghats using a combination of characters (Hora 1938;
Silas 1951; Talwar & Jhingran 1991; Jayaram 2009; Gopi 2010). Glyptothorax poonaensisdiffers from other species known from the Western Ghats, namely G. anamalaiensis, G. annandalei,G. davissinghi,G.
kudremukhensis, G. lonah, G. trewavasaeand G.
madraspatanum, in having smooth skin as oppose to
tuberculated or granulated skins in all other species. Apart from the difference in the skin
character, G.
poonaensis can be easily differentiated from G. anamalaiensisin lacking (vs. having) three broad white bands on the body. Glyptothorax annandaleiand G.
davissinghi can be separated from G poonaensisin having plaited (vs. smooth) ventral surfaces of the paired fins. Glyptothorax poonaensiscan be differentiated from G. annandalei, G.
davissinghi, G. lonah, G. trewavasaeand G.
madraspatanum in having poorly developed (vs. well
developed) adhesive apparatus. Here, poorly developed adhesive thoracic apparatus can be defined as
consisting of ridges of skin with only 16 prominent striae and only anterior
striae well defined while posterior striae indistinct, as oppose to well
developed adhesive thoracic apparatus with more then 20 striae and both
anterior and posterior striae distinctly visible. Other Glyptothoraxspecies found in the Western Ghats, which have smooth skin, are G. houseiand G.
malabarensis. Glyptothorax poonaensisdiffers from G.
housei by a poorly developed (vs. well developed)
adhesive thoracic apparatus, smooth (vs. plaited) ventral surfaces of the
paired fins, shorter (vs. longer) maxillary and nasal barbels and dorsal fin
origin nearer to adipose fin than to tip of snout (vs. nearer to tip of snout
than adipose fin base). Glyptothorax
poonaensis differs from G. malabarensis in having the thoracic
adhesive apparatus poorly developed and longer than broad (vs. moderately
developed, pentagonal in shape and as long as broad).
Redescription of Glyptothorax poonaensis
Detailed morphometry of G. poonaensisis given in Table 4 and details of the body structure, head structure and
details of skin are presented in Images 1, 2 and 3 respectively. The redescription is based on the
freshly collected specimens as indicated in Table 4. The specimens deposited in WILD match exactly in morphology
the types (4 specimens bearing the registration number F12126/1) deposited at
the Zoological Survey of India, Kolkata.
Head depressed, body
subcylindrical. Dorsal profile
rises evenly from tip of snout to origin of dorsal fin then slopes gently
ventrally from origin of dorsal fin to end of caudal peduncle. Ventral profile flat from snout tip to
anal fin base, then slopes gently dorsally from anal fin base to end of caudal
peduncle. Anus and urogenital
openings located at vertical through middle of adpressed pelvic fin. Skin smooth on body and head but could
be wrinkled in some specimens occurring either as a preservation artifact or
molting (Image 3). Lateral line complete, mid-lateral in position.
Head depressed and
broad. Snout prominent. Anterior and posterior nares large and
separated only by base of nasal barbels. Gill opening broad, extending from immediately ventral to post-temporal
to isthmus. Bony elements of dorsal surface of head covered with thick, smooth
skin. Occipital process does not
reach base of dorsal fin. On
either side of supraoccipital process, supracleithrum forms two finger like
projections separated by large interspace as shown by Hora (1938: fig.
1c). Eye ovoid, located entirely on
dorsal half of head, its horizontal axis longest, its diameter 9.8-13.5% HL.
Barbels four pairs,
maxillary barbel long and slender extending to middle of pectoral fin
base. Nasal barbels slender,
barely reaching eye. Inner
mandibular barbel origin close to midline extending up to gill opening on
ventral surface. Outer mandibular
barbel originating posteriolateral of inner mandibular barbel extending to
origin of pectoral fin. Mouth
inferior, premaxillary tooth band partially exposed when mouth is closed. Oral teeth small and villiform in
irregular rows, premaxillary teeth in single broad semilunate band. Dentary teeth in two narrow crescentic
bands separated at midline.
Dorsal fin located above
anterior third of body with I,5 (N=1) or I,6 (N=10) rays; fin margin convex;
spine short and gently curved. Adipose fin with anterior margin concave. Caudal fin strongly forked with lower lobes slightly longer
than upper lobes. Procurrent rays
symmetrical, extending slightly anterior on fin base. Anal fin base ventral to adipose fin origin. Anal fin with concave anterior margin
and straight posterior margin with ii,8 (N=8) or ii,9 (N=3) rays. Pelvic fin origin slightly behind the
posterior end of dorsal fin base. Pelvic fin with slightly convex margin and i,5 rays. Tip of adpressed fin not reaching anal
fin origin but covers anus and urogenital area. Pectoral fin with I,7 (N=1) or I,8 (N=7) or I,9 (N=3) fin
rays posterior fin margin slightly convex. Anterior spine margin smooth,
posterior margin with 17–21 serrations.
Thoracic adhesive
apparatus present, weakly developed, forming a narrow band and somewhat V
shaped appearance (Image 2c), with median depression present on posterior half
and extending from isthmus to level of middle of pectoral fin. Median ridges oriented longitudinally,
ridges uninterrupted.
In 4% formaldehyde
dorsal and lateral surfaces of head grayish with yellow tinge. A dark gray patch on the dorsal profile
extending from occipital process to posterior base of adipose fin. Ventral surface yellow to pale
yellow. Dorsal fin gray with white
band in middle, pectoral, ventral and anal fins with yellow base and gray
tips. Caudal fin with black or dark
gray base followed by gray tips. Head and body studded with randomly-distributed minute black dots. On the lateral surfaces of the body
gray with yellow tinge sometimes give rise to brownish coloration.
Molecular phylogeny
ModelTest in MEGA 5
(Tamura et al. 2011) suggested that General Time Reversible + Gamma +
Proportion Invariant (GTR+G+I) model was the best fit model for nucleotide
substitution for our data and thus it was applied to generate phylogenetic
hypothesis. The results for
phylogenetic analysis based on maximum likelihood, maximum parsimony, minimum
evolution and neighbor joining are shown in Fig. 2. The phylogeny based on a combined analysis of all three data
partitions (16S rDNA, cyt-b and cox1) suggests that G. poonaensisis nested within a clade consisting of species from the northern part of the
Indian subcontinent and China (Fig. 2). Interestingly, G.
poonaensis and G. davissinghi, both Western Ghats
species, were separated by a larger distance suggesting that southern Indian Glyptothoraxdo not form a monophyletic group.
Molecular dating of
divergence times between G.
poonaensis and other northern Indian species suggests thatG. poonaensisdiverged from other northern Indian species between 1.9 to 2.5 million years
ago (Fig. 3). We used only cyt-b gene for molecular dating because (Peng et al.
2006) have considered cyt-b gene for their analysis and we are using same
reference for the calibaration. Further, our total data matrix consisting of
16S rDNA, cyt-b and cox1 had non uniform substitution rates. Also, we could not use 16S rDNA and
cox1 genes individually as their sequence length was small for statistical
analysis. However, for cyt-b the
sequence length was adequate for analysis and the null hypothesis of equal
evolutionary rate throughout the
tree was not rejected (p=0.2065) making the molecular dating more reliable.
Distribution and population status
The collection locality
of one paratype of G.
poonaensis is Kharadigaon (18.5450N &
73.9490E), Pune (Hora 1938; Fraser 1942), while the type locality
(where three paratypes were also collected) is more vague, being merely given
as “near Poona [=Pune]”. We collected the species from the Village Markal
(18.6710N & 73.9810E) situated on the right bank of
Indrayani River, Pune. The two
collection sites are numbered 1 and 2 respectively in Fig. 4 and the associated
river basins are highlighted in blue. The total area drained by the two river basins is 6105.4km2. However, it is suspected that the
species could be locally extinct from the Mula-Mutha River (Kharat et al.
2003), indicating that the current extent of occurrence (IUCN 2001) of this
fish could be reduced by at least half. We lack estimates of the population size of this species, but it is
possible that it is relatively rare. This is based on the fact that in the extensive survey of Mula-Mutha
River by Fraser (1942) only one specimen was collected. Further, previous study on Indrayani
River (Yazdani & Mahabal 1976) failed to collect this species. Our discussions with the fishermen
revealed that this species, locally called as Patthar-chatu, is found only during
summer and early rainy season when the water level is low and even during this
period the fish is very rare. However, since directed fishing efforts for this particular species are
not done, we do not have exact estimates for the species abundance.
Biology
The length weight
relation of unsexed G.
poonaensis can be described by the equation W=0.0087L3.2436(Fig. 5, R2 = 0.933, p < 0.001). Since, the 95% confidence
interval of scaling exponent was in the range 2.5882-3.8989, the null
hypothesis, stated as the found value of the exponent is not significantly
different from the predicted value of 3 by isometry, could not be
rejected. Thus the fish grows
isometrically. Gut content of
three specimens of G.
poonaensis suggested that the fish feeds on benthic
macroinvertebrates such as freshwater prawns (Image 4A), maxillopod crustacean
(Branchiura) (Image 4B) and Odonata nymph (Image 4C). Our collection of gravid females suggests that this species
probably breeds during June to August (the monsoon season). Similar observations are also made on
other species of Glyptothorax(Dobriyal & Singh 1993; Nath 1994; Kaul 1994). Based on the fact that Glyptothorax are known to migrate
downstream for breeding (Kaul 1994), we suspect that Markal could be the
breeding ground for G.
poonaensis. This is because Markal is located on the main river and there are no
hill streams in its immediate vicinity. Further, according to the local fishermen, the species is found in
Markal area only during June to August, which is thought to be the breeding
period of the fish.
Threats and conservation measures
There are several
threats to G.
poonaensis in its known range. Important threats to hill stream species like G. poonaensisinclude alteration of hydrological regimes because of damming, increasing
pollution, deforestation leading to siltation, and introduced fish
species. Dams cut the flow of
water and lessen the speed of water creating semi-lacustrine conditions. These conditions may be highly disliked
by hill stream fishes like Glyptothorax,which are specialized fro living in torrent streams (Hora 1930). Also, Glyptothorax utilize gravel bed
areas for spawning which are lost in rivers immediately below the dams (Kaul
1994; Nath 1994). Further, impeded
water flow (both upstream and downstream of the dam) can lead to eutrophication
and the creation of oxygen-poor habitats. As species of genus Glyptothoraxrequire large amounts of oxygen in water (Hora 1930) such habitat alterations
are unsuitable for the species. Similar habitat alterations can also result
from urbanization leading to organic pollution in rivers. Such changes in the rivers near the
study area and their possible effects on the fish fauna are already documented
(Kharat et al. 2003; Wagh & Ghate 2003). As stated before, we suspect that Indrayani River at Markal
could be the breeding ground for G. poonaensis as Glyptothoraxspecies are known to migrate downstream for breeding (Kaul 1994). If this is true then pollution in this
area, which is an ongoing threat, is of major concern. An ongoing threat in terms of
deforestation, leading to siltation, can also affect the breeding grounds of
the fish. Recently we also observed
sandmining on large scale at Markal (Image 5), which is likely to affect the
habitat of G.
poonaensis.
Another major threat to
the species could be the presence of introduced exotic fish. Introduced exotic fishes have been
documented as major threats to fishes in the peninsular India (Kharat et al.
2003; Daniels 2006; Raghavan et al. 2008; Knight 2010). Kharat et al. (2003) have argued that
introduced fish like Oreochromis
mossambica, Poecilia reticulata, Gambusia affinis, Heteropneustes
fossilis, etc. in Mula-Mutha River, are threatening the
existence of many native fishes and might have caused even local extinction of
some species including G.
poonaensis. Even in Indrayani River at Markal we recorded alien species such as Oreochromis mossambicaand Clarias
gariepinus. Although direct evidence is lacking we suspect that at least Clarias gariepinusis a potential threat to the current population of G. poonaensisas it is a voracious predator (Krishnakumar et al. 2011). Restricted distribution of the speceis,
decline in the extent of occurance, ongoing threats to the habitats and
possible threats to the speceis justifies the IUCN Red list threat catagory of
this species as Endangered under the criteria B2ab(i,ii,iii,iv) as assessed by
Dahanukar (2010).
The potential breeding
grounds of the fish at Markal and the upstreams of Indrayani River, especially
hill streams in the adjoining hilly areas need protection. Halting of siltation by re-plantations
and avoidance of pollution could be helpful in saving the breeding grounds. Management of the introduced fishes,
especially Clarias
gariepinus, by controlled eradication of escaped stock and
increasing public awareness for avoiding further introductions (Sato et al.
2010), could also be helpful.
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