Comparative study of morphology and keratin levels in hair from deer and goat
Main Article Content
Abstract
Hair is a defining character of mammals. In the present study, the hair samples of Chital Axis axis, Sambar Deer Rusa unicolor, and goat Capra hircus were collected from the back, neck, abdomen and tail regions of carcasses brought to the forensic laboratory for necropsy examinations. Cross-sections of hair, cuticle scale, and medullary patterns were analyzed to establish indices for species identification. Keratin levels were also analyzed by protein electrophoresis (SDS-PAGE). We determined that both microscopic and SDS-PAGE analysis of guard hair is useful for identifying species, particularly in forensic applications.
Article Details
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors own the copyright to the articles published in JoTT. This is indicated explicitly in each publication. The authors grant permission to the publisher Wildlife Information Liaison Development (WILD) Society to publish the article in the Journal of Threatened Taxa. The authors recognize WILD as the original publisher, and to sell hard copies of the Journal and article to any buyer. JoTT is registered under the Creative Commons Attribution 4.0 International License (CC BY), which allows authors to retain copyright ownership. Under this license the authors allow anyone to download, cite, use the data, modify, reprint, copy and distribute provided the authors and source of publication are credited through appropriate citations (e.g., Son et al. (2016). Bats (Mammalia: Chiroptera) of the southeastern Truong Son Mountains, Quang Ngai Province, Vietnam. Journal of Threatened Taxa 8(7): 8953–8969. https://doi.org/10.11609/jott.2785.8.7.8953-8969). Users of the data do not require specific permission from the authors or the publisher.
References
Bahuguna, A., A. Sahajpal, S.P. Goyal, S.K. Mukharjee & V. Thakur (2010). Species identification from guard hair of selected Indian Mammals: A reference Guide, Wildlife Institute of India, Dehradun, 423 pp.
Brunner, H & B. Coman (1974). The identification of mammalian hair. Inkata Press, Melbourne, 50 pp. https://doi.org/10.1017/S0030605300014253
Caroline, S., M. Wadsley, M. Jolon, Dyer, S. Clerens, J. Matthew, Collins & J. Plowman (2013). Characterisation of novel α-keratin peptide markers for species identification in keratinous tissues using mass spectrometry. Rapid Communication in Mass Spectrometry. 27(23): 2685–2698. https://doi.org/10.1002/rcm.6730
Cornally, A & C. Lawton (2016). A guide to the identification of Irish mammal hair. Irish Wildlife Manuals, No. 92. National Parks and Wildlife Service, Department of the Arts, Heritage, Regional, Rural and Gaeltacht Affairs, Ireland.
Cortellini, V., A. Carobbio, G. Brescia, N. Cerri & A. Verzeletti (2019). A
comparative study of human and animal hairs: Microscopic hair comparison and cytochrome c oxidase I species identification. Journal of Forensic Science and Medicine 5: 20–30.
Deedrick, D.W & S.L. Koch (2004). Microscopy of Hair Part II: A practical guide and manual for animal hairs. Forensic Science Communications 6(3): 1–32.
Nakamura, A., M. Arimoto, K. Takeuchi & T. Fujii (2002). A rapid extraction procedure of human hair proteins and identification of phosphorylated species. Biological and Pharmaceutical Bulletin 25: 569–572. https://doi.org/10.1248/bpb.25.569
Folin, M & E. Contiero (1996). Electrophoretic analysis of mammalian hair keratins. Anthropologischer Anzeiger 54: 331–339.
Fuji, T., S. Takayama & I. Yumiko (2013). A novel purification procedure for keratin-associated proteins and keratin from human hair. Journal of Biological Macromolecules 13(3): 92–106. https://doi.org/10.14533/jbm.13.92
Gillespie, J.M. (1990). The proteins of hair and other hard α-keratins, pp. 95–128. In: Goldman R.D. & P.M. Steinert (eds). Cellular and Molecular Biology of Intermediate Filaments. Plenum Press, New York. https://doi.org/10.1007/978-1-4757-9604-9_4
Knecht, L. (2012). The use of hair morphology in the identification of mammals, pp. 129–43. In: Huffman, J.E. & J.R. Wallace (eds.). Wildlife Forensics: Methods and Applications. Wiley-Blackwell, Chichester, UK.
Langbein, L., M.A. Rogers, H. Winter, S. Praetzel & J. Schweizer (2001). The catalog of human hair keratins II. Journal of Biological Chemistry 276: 35123–35132. https://doi.org/10.1074/jbc.M103305200
Sahajpal, V., S.P. Goyal, R. Raza & R. Jayapal (2009). Identification of mongoose (genus: Herpestes) species from hair through band pattern studies using discriminate functional analysis (DFA) and microscopic examination. Science & Justice 49(3): 205–209. https://doi.org/10.1016/j.scijus.2008.09.002
Tridico, S. (2005). Examination, analysis, and application of hair in forensic science - animal hair. Forensic Science Review 17(1): 17–28.