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A cross-sectional, coprological survey of gastrointestinal (GI) parasites of wild mammals in four major National Parks in Sri Lanka: Wilpattu, Udawalawe, Wasgamuwa, and Horton Plains was carried out during November 2016 to August 2017. Fresh fecal samples were collected and analyzed using sedimentation technique, iodine & saline smears, and Sheather’s sucrose flotation for morphological identification parasite eggs, cysts, and larvae. A modified salt flotation was carried out for egg counts. Seventy samples from 10 mammal species: Asian Elephant, Spotted Deer, Water Buffalo, Sambar, Indian Hare, Asian Palm Civet, Sloth Bear, Wild Boar, Grey Langur, Leopard, and four unknown mammals (two carnivores, one herbivore and one omnivore) were analyzed. Most were infected (94.3%) with more than one GI parasites. The highest prevalence of infection was recorded in Horton Plains (100%), followed by Wasgamuwa (92.8%), Wilpattu (90.4%) and Udawalawe (75.0%) with a significant difference among four parks (Chi square test; χ2=35.435; df=3; p<0.001). Nineteen species of GI parasites were recorded, of which Entamoeba, Isospora, Balantidium, Fasciola, Moniezia, Dipylidium, strongyles, Toxocara, Trichiurus and hookworms were the most common. Strongyles (62.1%) and Entamoeba (80.3%) were the most prevalent helminth and protozoan infections, respectively. Overall, there was no difference in the prevalence of protozoans (84.3%) and helminths (87.1%; χ2=1.0; df=1; p=0.317). In carnivores, Entamoeba, Balantidium, Moniezia, strongyles and Strongyloides were common and in herbivores, Entamoeba, strongyles, Strongyloides and Toxocara were common. The quantitative analysis showed strongyles (17.639 EPG) and Isospora (18,743 OPG) having the highest infection intensity among helminthes and protozoans, respectively. This study provides baseline information of GI parasites and their distribution in wild mammals in the four national parks. Although the prevalence of GI infections was high, their intensity shows that they could be incidental infections. When the prevalence of an infection is high but the intensity is low, it is unlikely to be a major health problem leading to the endangerment of a species. Parasitic diseases can not only affect conservation efforts, but they are also natural selection agents and drive biological diversification, through influencing host reproductive isolation and speciation.
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Achhami, B., H.P. Sharma & A.B. Bam (2016). Gastro-intestinal parasites of Musk Deer (Moschus chrysogaster Hodgson, 1839) in Langtang National Park, Nepal. Journal of Institute of Science and Technology 21(1): 71–75.
Adeniyi, I.C., O.A. Morenikeji & B.O. Emikpe (2015). The prevalence of gastro-intestinal parasites of carnivores in university zoological gardens in South West Nigeria. Journal of Veterinary Medicine and Animal Health 7(4): 135–139.
Altizer, S., C.L. Nunn, P.H. Thrall, J.L. Gittleman, J. Antonovics, A.A. Cunningham, A.P. Dobson, V. Ezenwa, K.E. Jones, A.B. Pedersen & M. Poss (2003). Social organization and parasite risk in mammals: integrating theory and empirical studies. Annual Review of Ecology, Evolution, and Systematics 34(1): 517–547.
Anderson, R.M. (1979). Parasite pathogenecity and the depression of the host population equilibria. Nature 279: 150–152.
Apio, A., M. Plath & T. Wronski (2006). Patterns of gastrointestinal parasitic infections in the Bushbuck, Tragelaphus scriptus from the Queen Elizabeth National Park, Uganda. Journal of Helminthology 80: 213–218.
Aviruppola, A.M.J.K., R.S. Rajakaruna & R.P.V.J. Rajapakse (2016). Coprological survey of gastrointestinal parasites of mammals in Dehiwala National Zoological Gardens, Sri Lanka. Ceylon Journal of Science 45(1): 83–96.
Best, A., A. White & M. Boots (2014). The co-evolutionary implications of host tolerance. Evolution; International Journal of Organic Evolution 68: 1426–1435
Bezjian, M., T.R. Gillespie, C.A. Chapman & E.C. Greiner (2008). Coprologic evidence of gastrointestinal helminths of forest baboons, Papioanubis, in Kibale National Park, Uganda. Journal of Wildlife Diseases 44(4): 878–887.
Blanchet, S., O. Rey, P. Berthier, S. Lek & G. Loot (2009). Evidence of parasite mediated disruptive selection on genetic diversity in a wild fish population. Molecular Ecology 18: 1112–1123.
Blehert, D.S., A.C. Hicks, M. Behr, C.U. Meteyer, B.M. Berlowski-Zier, E.L. Buckles, J.T.H. Coleman, S.R. Darling, A. Gargas, R. Niver, J.C. Okoniewski, R.J. Rudd & W.B. Stone (2009). Bat white-nose syndrome: an emerging fungal pathogen? Science 323–227.
Borkovcova, M. & J. Kopirova (2005). Parasitic helminthes of reptiles (Reptilia) In South Moravia (Czech Republic). Parasitology Research 95(1): 77–80.
Chattopadhyay, A.K. & S. Bandyopadhyay (2013). Seasonal variations of EPG levels in gastro-intestinal parasitic infection in a Southeast Asian controlled locale: a statistical analysis. Springerplus 2: 2–9.
Coffey, R., E. Cummins, M. Cormican, V.O. Flaherty & S. Kelly (2007). Microbial exposure assessment of waterborne pathogens. Human Ecological Risk Assessment 13: 1313–1351.
Cordon, G.P., A.H. Prados, D. Romero, S.M. Moreno, A. Pontes, A. Osuna & M.J. Rosales (2008) Intestinal parasitism in the animals of the zoological garden ‘‘Pena Escrita’’ (Almunecar, Spain). Veterinary Parasitology 156: 302–309.
Dahlberg, A., R. Rohder & K. Sandell (2010).national parks and environmental justice: comparing access rights and ideological legacies in three countries. Conservation and Society 8(3): 209–224.
Dawet, A., D.P. Yakubu & H.M. Butu (2013). Survey of gastrointestinal parasites of non-human primates in Jos Zoological Garden. Journal of Primatology 2(1): 1–3.
De Castro, F. & B.M. Bolker (2005). Parasite establishment and host extinction in model communities. Oikos 111(3): 501–513.
Dunn, A.M. (1978). Veterinary Helminthology. 2nd Edition. William Heinemann Medical Books, London, UK.
Engh, A.L., K.G. Nelson, R. Peebles, A.D. Hernandez, K.K. Hubbard & K.E. Holekamp (2003). Coprologic Survey of Parasites of Spotted Hyenas (Crocuta crocuta) in the Masai Mara National Reserve, Kenya. Journal of Wildlife Diseases 39(1): 224–227.
Freeland, W.J., B.L.J. Delvinqueir& B. Bonnin (1986). Food and parasitism of the Cane Toad, Bufo marinus, in relation to time since colonization. Wildlife Research 13(3): 489–499.
Gillespie, T.R., E.C. Greiner & C.A. Chapman (2004). Gastrointestinal parasites of the guenons of western Uganda. Journal of Parasitology 90: 1356–1360.
Gillespie, T.R., E.C. Greiner & C.A. Chapman (2005). Gastrointestinal parasites of the colobus monkeys of Uganda. Journal of Parasitology 91: 569–573.
Gómez, A. & E. Nichols (2013). Neglected wild life: parasitic biodiversity as a conservation target. International Journal for Parasitology: Parasites and Wildlife 2: 222–227.
Gracenea, M., M.S. Gomez, J. Torres, E. Carne & J. Fernadez-Moran (2002) Transmission dynamics of Cryptosporidium in primates and herbivores at the Barcelona Zoo: A long-term study. Veterinary Parasitology 104: 19–26.
Kaffashi, S., A. Radam, M.N. Shamsudin, M.R. Yacob & N.H. Nordin (2015). Ecological Conservation, Ecotourism, and Sustainable Management: The Case of Penang National Park. Forests 6: 2345–2370.
Karesh, W.B., A. Dobson, J.O. Lloyd-Smith, J. Lubroth, M.A. Dixon, M. Bennett, S. Aldrich, T. Harrington, P. Formenty, E.H. Loh & C.C. Machalaba (2012). Ecology of zoonoses: natural and unnatural histories. The Lancet 380(9857): 1936–1945.
Kloch, A., M. Bednarska & A. Bajera (2005). Intestinal macro and microparasites of Wolves (Canis lupus L.) from north-eastern Poland Recovered by Coprological Study. Annals of Agricultural and Environmental Medicine 12: 237–245.
Kobbekaduwa, V., C. Fillieux, A. Thududgala, R.P.V.J. Rajapakse & R.S. Rajakaruna (2017). First record of tapeworm Moniezia (Cestoda: Anoplocephalidae) infections in Leopards: coprological survey of gastrointestinal parasites of wild and captive cats in Sri Lanka. Journal of Threatened Taxa 9(3): 9956–9961. https://doi.org/10.11609/jott.2918.104.22.16856-9961
Lively, C.M., M.F. Dybdahl, J. Jokela, E.E. Osnas & L.F. Delph (2004). Host sex and local adaptation by parasites in a snail-trematode interaction. The American Naturalist 164: S6–S18.
Marathe, R. R., S.S. Goel, S.P. Ranade, M.M. Jog & M.G. Watwe (2002). Patterns in abundance and diversity of fecally dispersed parasites of tiger in Tadoba National Park, Central India. BMC Ecology 2: 1–10.
Marcogliese, D.J. (2004). Parasites: small players with crucial roles in the ecological theater. EcoHealth 1: 151–164.
McCallum, H. & A. Dobson (1995). Detecting disease and parasite threats to endangered species and ecosystems. Trends in Ecology and Evolution 10: 190–194.
Muehlenbein, M.P. (2005). Parasitological analyses of the Male Chimpanzees (Pantroglodytes schweinfurthii) at Ngogo, Kibale National Park, Uganda. American Journal of Primatology 65: 167–179.
Muller-Graf, C.D.M. (1995). A coprological survey of intestinal parasites of Wild Lions (Panthera leo) in the Serengeti and the Ngorongoro Crater, Tanzania, East Africa. The Journal of Parasitology 81(5): 812–814.
Okulewicz, A., A. Perec-Matysiak, K. Bunkowska & J. Hildebrand (2012). Toxocara canis, Toxocara cati and Toxascaris leonina in wild and domestic carnivores. Helminthologia 49(1): 3–10.
Pettifer, H.L. (1984). The helminth fauna of the digestive tracts of Chacma Baboons, Papio ursinus, from different localities in the Transvaal. Onderstepoort Journal of Veterinary Research 51: 161–170.
Preston, D. & P. Johnson (2010). Ecological Consequences of Parasitism. Nature Education Knowledge 3(10): 47.
Rausher, M.D. (2001). Co-evolution and plant resistance to natural enemies. Nature 411: 857–864.
Rosenthal, J. (2010). Climate Change and Geographic Distribution of Infectious Diseases. EcoHealth 6: 489-495.
Roy, B.A. & J.W. Kirchner (2000). Evolutionary dynamics of pathogen resistance and tolerance. Evolution 54: 51–63.
Sallows, T.A. (2007). Diet preference and parasites of grey wolves in Riding Mountain National Park of Canada. M.Sc. Thesis. University Manitoba, Canada.
Schuster, F.L. & L. Ramirez-avila (2008). Current world status of Balantidium coli. Clinical Microbiology Reviews 21(4): 626–638.
Sinclair, A.R.E. & M.N. Griffith (1979). Serengeti: Dynamics of an Ecosystem. Chicago: University of Chicago Press.
Singh, P., M.P. Gupta, L.D. Singla, N. Singh & D.R. Sharma (2006a). Prevalence and chemotherapy of gastrointestinal helminthic infections in wild carnivores of Mahendra Choudhury Zoological Park, Punjab. Journal of Veterinary Parasitology 20(1): 17–23.
Singh, P., M.P. Gupta, L.D. Singla, S. Sharma, B.S. Sandhu & D.R. Sharma (2006b). Parasitic infections in wild herbivores in the Mahendra Choudhury Zoological Park, Chhatbir, Punjab. Zoo’s Print Journal 21(11): 2459–2461. https://doi.org/10.11609/JoTT.ZPJ.1519.2459-61
Stuart, P., O. Golden, A. Zintl, T.D. Waal, G. Mulcahy, E. McCarthy & C. Lawton (2017). A coprological survey of parasites of wild carnivores in Ireland. Parasitology Research: 1–10.
Summers, K., S. McKeon, J. Sellars, M. Keusenkothen, J. Morris, D. Gloeckner, C. Pressley, B. Price & H. Snow (2003). Parasitic exploitation as an engine of diversity. Biological Reviews 78: 639–675.
Sumption , K.J. & J.R. Flowerdew (1985). The ecological effects of the decline in Rabbits (Oryctolagus cuniculus L.) due to myxomatosis. Mammal Review 15(4): 151–186.
Tandon, V., P.K. Kar, B. Das, B. Sharma & J. Dorjee (2005). Preliminary survey of gastro-Intestinal helminth infection in herbivores livestock of mountainous regions of Bhutan and Arunachal Pradesh. Zoos’ Print Journal 20(5): 1867–1868. https://doi.org/10.11609/JoTT.ZPJ.1227.1867-8
Turner, W.C., W.D. Versfeld, J.K. Kilian & W.M. Getz (2012). Synergistic effects of seasonal rainfall, parasites and demography on fluctuations in springbok body condition. Journal of Animal Ecology 81: 58–69. https://doi.org/10.1111/j.1365-2656.2011.01892.x
Vale, P.F., A. Fenton & S.P. Brown (2014). Limiting damage during infection: lessons from infection tolerance for novel therapeutics. Public Library of Science 12: e1001769.
Weerakoon, D. (2012). The Taxonomy and Conservation Status of Mammals in Sri Lanka. Available from: https://www.researchgate.net/publication/268630003 Accessed on 26 December 2019.
Wegner, K.M., T.B.H. Reusch & M. Kalbe (2003). Multiple parasites are driving major histocompatibility complex polymorphism in the wild. Journal of Evolutionary Biology 16: 224– 232.
Zajac, A.M. & G.A. Conboy (2012). Fecal examination for the diagnosis of parasitism, pp. 3–14. In: Zajac, A.M. & G.A. Conboy (eds.). Veterinary Clinical Parasitology. John Wiley & Sons, 354pp.