Table 1. Effect of medium on spore germination, development of gametophyte and sporophyte and field establishment in Pronephrium triphyllum

Medium & pH	% of Germination ± S.D.	% of Prothalli formation ± S.D.	% of Sporophyte formation ± S.D.	Mean no. of croziers/ prothallus ± S.D.	Mean length of Sporophyte ± S.D.	% of establishment in polycups ± S.D.	% of establishment in Pots ± S.D.	% of establishment in field ± S.D.
KC Solid 5.8	15.3±1.27	67.3±1.31	-	-	-	-	-	-
KN Solid 5.8	38.3±1.13	72.3±1.08	-	-	-	-	-	-
Mi Solid 5.5	21.3±0.83	81.3±1.31	-	-	-	-	-	-
MO Solid 5.5	22.3±0.81	68.4±1.34	-	-	-	-	-	-
KC Liquid	-	-	16.5±1.31	1.78±0.34	1.12±0.16	73.5±1.24	78.4±1.31	69.3±1.21
KN Liquid	-	-	52.3±1.43	4.0±1.63	1.38±0.74	82.3±1.31	79.3±1.31	72.3±1.24

Table 2. Effect of medium on spore germination, gametophyte, sporophyte formation and field establishment of Sphaerostephanos unitus

Medium	% of Germination ± S.D.	% of Prothalli formation ±_S.D.	% of Sporophyte formation ±_S.D.	Mean no. of croziers/ prothallus ± S.D.	Mean length of Sporophyte ±_S.D.	% of establishment in polycups ± S.D.	% of establishment in Pots ± S.D.	% of establishment in field ± S.D.
KN Solid	36.8±1.31	73.8±1.31	76.8±1.41	3.2±1.13	6.3±1.38	79.8±1.34	83.1±1.21	74.8±1.31
KC Solid	26.3±1.30	74.8±1.28	-	-	-	-	-	-
MS Solid	-	-	-	-	-	-	-	-
Mitra	34.8±1.21	66.3±1.21	-	-	-	-	-	-

Table 3. Growth of micropropagated plants of P. triphyllum and S. unitus re-established into Kodaikannal Botanic Garden at Kodaikanal, Tamil Nadu, India

Plant species	No. of plants transferred to polycups	% of establishing in polycup	No. of plants transferred to pots	% of establishment in pots	No. of plants transferred to field	% of establishment
P. triphyllum	375	73.5	275	77	120	85
S. unitus	300	79	235	73	120	75