Image 1. Malformations induced in the host due to the infection of U. hobsoni (a-d);

Callus induction from uninfected (e) and infected (f) leaves of J. officinale var. grandiflorum;

Photomicrograph of the callus derived from uninfected (g) and infected (h) leaves.

Note the haustoria and intercellular hyphae clearly distinguishable in the callus derived

from the infected leaves (arrows) (© Aniket A. Kuvalekar)

 

 

 

Table 1. Fresh weight and dry weight of the calli initiated from the healthy and infected explants.

 The calli from the infected explants grew faster but seized the growth after 32nd day post callus initiation.

The calli from the healthy tissues grew slowly but continued the growth beyond the reported time frame.

At each reported time period, the calli from eight different explants of each sample were used to determine the growth

 

Days post callus initiation

 

Callus from healthy explants

 

Callus from infected explants

 

Fresh Weight (g)

 

Dry Weight (g)

 

Fresh Weight (g)

 

Dry Weight (g)

 

4

 

0.005

 

0.001

 

0.004

 

0.001

 

8

 

0.008

 

0.003

 

0.009

 

0.005

 

12

 

0.011

 

0.005

 

0.014

 

0.010

 

16

 

0.014

 

0.010

 

0.019

 

0.014

 

20

 

0.018

 

0.012

 

0.026

 

0.024

 

24

 

0.022

 

0.018

 

0.032

 

0.029

 

28

 

0.026

 

0.023

 

0.035

 

0.030

 

32

 

0.028

 

0.024

 

0.035

 

0.031